AB228847

Anti-Cardiac Troponin I antibody

Name: Anti-Cardiac Troponin I antibody (ab228847) | Abcam
Brand: Abcam Limited
SKU: AB228847
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Cardiac Troponin I antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TNNI3.

View Alternative Names

TNNC1, TNNI3, Cardiac troponin I

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (AB228847)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (AB228847)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cardiac Troponin I (green) using ab228847 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain : Hoechst 33342 (blue).

Supplier Data

Western blot - Anti-Cardiac Troponin I antibody (AB228847)

12% SDS-PAGE

All lanes:

Western blot - Anti-Cardiac Troponin I antibody (ab228847) at 1/1000 dilution

All lanes:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

Predicted band size: 24 kDa

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Supplier Data

Western blot - Anti-Cardiac Troponin I antibody (AB228847)

12% SDS-PAGE

All lanes:

Western blot - Anti-Cardiac Troponin I antibody (ab228847) at 1/5000 dilution

All lanes:

Mouse heart whole cell lysate at 50 µg

Predicted band size: 24 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TNNI3. The exact immunogen used to generate this antibody is proprietary information.

P19429

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

See full target information TNNI3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:108096 PubMed38222111

2024

Human iPSC modeling recapitulates sympathoadrenal development and reveals an aberrant developmental subpopulation in familial neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphane Van Haver,Yujie Fan,Sarah-Lee Bekaert,Celine Everaert,Wouter Van Loocke,Vittorio Zanzani,Joke Deschildre,Inés Fernandez Maestre,Adrianna Amaro,Vanessa Vermeirssen,Katleen De Preter,Ting Zhou,Alex Kentsis,Lorenz Studer,Frank Speleman,Stephen S Roberts

Microsystems & nanoengineering 9:57 PubMed37180453

2023

Evaluating the efficacy and cardiotoxicity of EGFR-TKI AC0010 with a novel multifunctional biosensor.

Applications

Unspecified application

Species

Unspecified reactive species

Deming Jiang,Xinwei Wei,Yuxuan Zhu,Yong Qiu,Xin Liu,Liubing Kong,Fengheng Li,Jingwen Liu,Liujing Zhuang,Hao Wan,Kejing Ying,Ping Wang

Frontiers in oncology 12:977251 PubMed36727078

2023

Integrated analysis of genome-wide DNA methylation and cancer-associated fibroblasts identified prognostic biomarkers and immune checkpoint blockade in lower grade gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Dong,Fang Wang,Xin Gao,Hongtao Zhao,Jiheng Zhang,Nan Wang,Zhihui Liu,Xiuwei Yan,Jiaqi Jin,Yixu Ba,Shuai Ma,Jianyang Du,Hang Ji,Shaoshan Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cardiac Troponin I antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (AB228847)

Western blot - Anti-Cardiac Troponin I antibody (AB228847)

Western blot - Anti-Cardiac Troponin I antibody (AB228847)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise