Rabbit Polyclonal Cardiac Troponin I antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TNNI3.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TNNI3. The exact immunogen used to generate this antibody is proprietary information. Database link P19429
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Cat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Cat, Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Cat, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Alternative names
Cardiac troponin I, TNNI3, TNNC1
Recommended products
Rabbit Polyclonal Cardiac Troponin I antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TNNI3.
Alternative names
Cardiac troponin I, TNNI3, TNNC1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TNNI3. The exact immunogen used to generate this antibody is proprietary information. Database link P19429
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (ab228847)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cardiac Troponin I (green) using ab228847 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
Western blot - Anti-Cardiac Troponin I antibody (ab228847)
12% SDS-PAGE
All lanes: Western blot - Anti-Cardiac Troponin I antibody (ab228847) at 1/1000 dilution
All lanes: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Western blot - Anti-Cardiac Troponin I antibody (ab228847)
12% SDS-PAGE
All lanes: Western blot - Anti-Cardiac Troponin I antibody (ab228847) at 1/5000 dilution
All lanes: Mouse heart whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
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