AB47003

Anti-Cardiac Troponin I antibody

Name: Anti-Cardiac troponin I antibody, rabbit polyclonal (ab47003) | Abcam
Brand: Abcam Limited
SKU: AB47003
Price: 450 USD
Availability: InStock
Rating: 5 (17 reviews)

BOND RX™ Validated
What is this?

(17 Reviews)

(174 Publications)

Anti-Cardiac Troponin I antibody (ab47003) is a rabbit polyclonal antibody detecting Cardiac Troponin I in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Human, Mouse, Pig, Rat.

- Over 140 publications
- Trusted since 2007

View Alternative Names

TNNC1, TNNI3, Cardiac troponin I

6 Images

Project3427****

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

All lanes:

Western blot - Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/mL

Lane 1:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 2:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 24 kDa

Observed band size: 26 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (AB47003)

ICC/IF

AbReview29464****

Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (AB47003)

ab47003 staining Cardiac Troponin I in Human cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted FITC-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Lab

Immunohistochemistry - Anti-Cardiac Troponin I antibody (AB47003)

Immunohistochemical analysis of formalin fixed paraffin embedded human heart labelling cardiac Troponin I with ab47003 at a concentration of 0.5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

ab47003 anti-Cardiac Troponin I antibody was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (AB47003)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (AB47003)

IHC image of Cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47003, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

AbReview15169****

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

Blocking Step : 20% Serum for 3 hours at 24°C

All lanes:

Western blot - Anti-Cardiac Troponin I antibody (ab47003) at 1/20000 dilution

All lanes:

Pig Heart whole cell lysate at 4 µg

Secondary

All lanes:

An HRP-conjugated Goat anti-rabbit polyclonal at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 29 kDa

true

This image is courtesy of an Abreview submitted by Dr. Mario Torrado

Unknown

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

All lanes:

Western blot - Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/mL

Lane 1:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 2:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 3:

Heart (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 24 kDa

Observed band size: 160 kDa,26 kDa,80 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

ICC/IF, WB, IHC-P, Flow Cyt, ELISA, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Anti-Cardiac Troponin I antibody (ab47003) is a rabbit polyclonal antibody and is validated for use in ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB in african green monkey, human, mouse, rat samples.

Anti-Cardiac Troponin I antibody (ab47003) has been cited over 147 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Cardiac Troponin I antibody (ab47003) has high sensitivity and specificity.

Anti-Cardiac Troponin I antibody (ab47003) has 17 independent reviews from customers.

Anti-Cardiac Troponin I antibody (ab47003) specifically detects Cardiac Troponin I (UniProt ID: P19429; Molecular weight: 24kDa) and is sold in a 100 µg selling size.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

See full target information TNNI3

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Publications (174)

Recent publications for all applications. Explore the full list and refine your search

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Phosphoserine aminotransferase 1 promotes serine synthesis pathway and cardiac repair after myocardial infarction.

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Species

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Ajit Magadum,Vandana Mallaredy,Rajika Roy,Darukeshwara Joladarashi,Charan Thej,Zhongjian Cheng,Maria Cimini,May Truongcao,Adam Chatoff,Claudia V Crispim,Vagner O C Rigaud,Carolina Gonzalez,Cindy Benedict,Celio X C Santos,Nathaniel W Snyder,Mohsin Khan,Ajay M Shah,Walter J Koch,Raj Kishore

Nature communications 16:6861 PubMed40715150

2025

Tumour initiated purinergic signalling promotes cardiomyocyte RBFOX1 degradation and cardiotoxicity from DNA damaging anticancer agents.

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Species

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Saymon Tejay,Maria Areli Lorenzana-Carrillo,Guocheng Huang,Seyed Amirhossein Tabatabaei Dakhili,Yuan -Yuan Zhao,Farah Eaton,Michelle Mendiola Pla,Dawn E Bowles,Adam Kinnaird,D Ian Paterson,Edith Pituskin,John R Ussher,Evangelos D Michelakis,Gopinath Sutendra

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Human induced pluripotent stem cell derived nanovesicles for cardiomyocyte protection and proliferation.

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Yuhua Wei,Xiaoxiao Geng,Qing You,Yu Zhang,Fangfang Cao,Gunaseelan Narayanan,Thanh Nguyen,Xiaoyuan Chen,Jianyi Zhang,Lei Ye

Biology direct 20:65 PubMed40414862

2025

Macrophage Notch1 drives septic cardiac dysfunction by impairing mitophagy and promoting NLRP3 activation.

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Unspecified application

Species

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Yanjun Zheng,Jingrong Lin,Guoqing Wan,Xuefeng Gu,Jian Ma

Communications biology 8:745 PubMed40360739

2025

AI-guided laser purification of human iPSC-derived cardiomyocytes for next-generation cardiac cell manufacturing.

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Prakaimuk Saraithong,Peyton Krajcarski,Yukako Kusaka,Moe Yamada,Junichi Matsumoto,Hailey Cunningham,Sama Salih,Darby Jones,Devika Baddhan,Christian Hausner,Justus Anumonwo,Anthony Rosenzweig,Mary M Navarro,Luis Villa Diaz,Joseph Criscione,Deok-Ho Kim,Todd J Herron

Nature cardiovascular research 4:397-411 PubMed40055464

2025

Genetic tracing and topography of spontaneous and stimulated cardiac regeneration in mice.

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Species

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Ilaria Secco,Ana Backovic,Mateusz Tomczyk,Antonio Mura,Gang Li,Francesca Bortolotti,Simone Vodret,Matteo Dal Ferro,Elena Chiavacci,Lorena Zentilin,Gianfranco Sinagra,Serena Zacchigna,Miguel Mano,Mauro Giacca

Materials today. Bio 31:101500 PubMed39944530

2025

Cardioprotective effects of hUCMSCs-Exosi-EGR1 MN patch in MI/RI by modulating oxidative stress and mitophagy.

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Unspecified application

Species

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Chen Huang,Xun Zhang,Shi-Xiong Wu,Qing Chang,Zhi-Kun Zheng,Jing Xu

STAR protocols 5:103461 PubMed39579351

2024

Protocol for isolating and culturing neonatal murine cardiomyocytes.

Applications

Unspecified application

Species

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Chiara Bongiovanni,Carmen Miano,Francesca Sacchi,Silvia Da Pra,Irene Del Bono,Stefano Boriati,Gabriele D'Uva

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cardiac Troponin I antibody

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (AB47003)

Immunohistochemistry - Anti-Cardiac Troponin I antibody (AB47003)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (AB47003)

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (174)

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