Anti-Cardiac Troponin I antibody ab47003 is a rabbit polyclonal antibody that is used in Cardiac Troponin I western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.
- One antibody for all your Cardiac Troponin I staining, use in Cardiac Troponin I western blotting, IHC, immunofluorescence and flow cytometry
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- Journal of extracellular vesicles 12:e123712023Engineered small extracellular vesicle-mediated NOX4 siRNA delivery for targeted therapy of cardiac hypertrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Young Kang,Dasom Mun,Yumin Chun,Da-Seul Park,Hyoeun Kim,Nuri Yun,Boyoung JoungPubMed 37795828
- Bioactive materials 28:206-2262023Dual human iPSC-derived cardiac lineage cell-seeding extracellular matrix patches promote regeneration and long-term repair of infarcted hearts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Jiang,Ling-Ling Zhang,Fan Zhang,Wei Bi,Peng Zhang,Xiu-Jian Yu,Sen-Le Rao,Shi-Hui Wang,Qiang Li,Chen Ding,Yin Jin,Zhong-Min Liu,Huang-Tian YangPubMed 37274446
- The Journal of clinical investigation 133:2023Pressure overload induces ISG15 to facilitate adverse ventricular remodeling and promote heart failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVeera Ganesh Yerra,Sri Nagarjun Batchu,Harmandeep Kaur,M D Golam Kabir,Youan Liu,Suzanne L Advani,Duc Tin Tran,Shadi Sadeghian,Phelopater Sedrak,Filio Billia,Uros Kuzmanov,Anthony O Gramolini,Deema O Qasrawi,Evgeniy V Petrotchenko,Christoph H Borchers,Kim A Connelly,Andrew AdvaniPubMed 37115698
- Vascular health and risk management 19:93-1012023Correlation Between Cardiac Index, Plasma Troponin I, Myocardial Histopathology, CPB and AoX Duration in Glutamine versus No Glutamine Administered Patients with Low Ejection Fraction Undergoing Elective On-Pump CABG Surgery: Secondary Analysis of an RCT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesI Made Adi Parmana,Cindy Elfira Boom,Lisnawati Rachmadi,Dudy Arman Hanafy,Yunita Widyastuti,Muchtaruddin Mansyur,Bambang Budi SiswantoPubMed 36880009
- Computational and structural biotechnology journal 21:1759-17732023Modeling incomplete penetrance in arrhythmogenic cardiomyopathy by human induced pluripotent stem cell derived cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarzia De Bortoli,Viviana Meraviglia,Katarina Mackova,Laura S Frommelt,Eva König,Johannes Rainer,Chiara Volani,Patrizia Benzoni,Maja Schlittler,Giada Cattelan,Benedetta M Motta,Claudia Volpato,Werner Rauhe,Andrea Barbuti,Serena Zacchigna,Peter P Pramstaller,Alessandra RossiniPubMed 36915380
- Circulation research 132:400-4142023CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEleder Cachorro,Mario Günscht,Mario Schubert,Mirna S Sadek,Johanna Siegert,Fabian Dutt,Carla Bauermeister,Susann Quickert,Henrik Berning,Felix Nowakowski,Simon Lämmle,Rebecca Firneburg,Xiaojing Luo,Stephan R Künzel,Erik Klapproth,Peter Mirtschink,Manuel Mayr,Matthias Dewenter,Christiane Vettel,Jordi Heijman,Kristina Lorenz,Kaomei Guan,Ali El-Armouche,Michael Wagner,Susanne KämmererPubMed 36715019
- Nature metabolism 5:129-1462023TREM2 resident macrophages protect the septic heart by maintaining cardiomyocyte homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Zhang,Yang Wang,Shiyu Chen,Jiali Mao,Yue Jin,Hui Ye,Yan Zhang,Xiwang Liu,Chenchen Gong,Xuejun Cheng,Xiaoli Huang,Andreas Hoeft,Qixing Chen,Xuekun Li,Xiangming FangPubMed 36635449
- The Journal of clinical investigation 132:2022Loss of function of the nuclear envelope protein LEMD2 causes DNA damage-dependent cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXurde M Caravia,Andres Ramirez-Martinez,Peiheng Gan,Feng Wang,John R McAnally,Lin Xu,Rhonda Bassel-Duby,Ning Liu,Eric N OlsonPubMed 36377660
- Developmental cell 57:2517-2532.e62022Endocardium-to-coronary artery differentiation during heart development and regeneration involves sequential roles of Bmp2 and Cxcl12/Cxcr4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaetano D'Amato,Ragini Phansalkar,Jeffrey A Naftaly,Xiaochen Fan,Zhainib A Amir,Pamela E Rios Coronado,Dale O Cowley,Kelsey E Quinn,Bikram Sharma,Kathleen M Caron,Alessandra Vigilante,Kristy Red-HorsePubMed 36347256
- Life (Basel, Switzerland) 12:2022Effects of Obesity and Diabesity on Ventricular Muscle Structure and Function in the Zucker Rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed Sultan,Ernest Adeghate,Bright Starling Emerald,Muhammad A Qureshi,Saeed Tariq Minhas,Frank Christopher HowarthPubMed 36013400
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| Flow Cyt | ELISA | WB | ICC/IF | IHC-Fr | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Expected | Expected | Tested | Tested | Expected | Tested |
| Mouse | Expected | Expected | Tested | Expected | Expected | Expected |
| Rat | Expected | Expected | Tested | Expected | Expected | Expected |
| Chimpanzee | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Pig | Expected | Expected | Tested | Expected | Expected | Expected |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Pig | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Pig | Dilution info 1/20000 | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Associated Products
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Target data
Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Alternative names
Cardiac troponin I, TNNI3, TNNC1
Recommended products
Anti-Cardiac Troponin I antibody ab47003 is a rabbit polyclonal antibody that is used in Cardiac Troponin I western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.
- One antibody for all your Cardiac Troponin I staining, use in Cardiac Troponin I western blotting, IHC, immunofluorescence and flow cytometry
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Western blot - Anti-Cardiac Troponin I antibody (ab47003)
All lanes: Western blot - Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/mL
Lane 1: Human heart tissue lysate - total protein (ab29431) at 10 µg
Lane 2: Human liver tissue lysate - total protein (ab29889) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (ab47003)
IHC image of Cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47003, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - Anti-Cardiac Troponin I antibody (ab47003)
All lanes: Western blot - Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/mL
Lane 1: Human heart tissue lysate - total protein (ab29431) at 10 µg
Lane 2: Heart (Mouse) Tissue Lysate at 10 µg
Lane 3: Heart (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 160 kDa, 26 kDa, 80 kDa
Exposure time: 3min
This image is courtesy of a customer review submitted by Dr. Mario TorradoWestern blot - Anti-Cardiac Troponin I antibody (ab47003)
Blocking Step: 20% Serum for 3 hours at 24°C
All lanes: Western blot - Anti-Cardiac Troponin I antibody (ab47003) at 1/20000 dilution
All lanes: Pig Heart whole cell lysate at 4 µg
SecondaryAll lanes: An HRP-conjugated Goat anti-rabbit polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 29 kDa
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (ab47003)
ab47003 staining Cardiac Troponin I in Human cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted FITC-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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