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AB10237

Anti-Cardiac Troponin I antibody [M155]

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(2 Publications)

Mouse Monoclonal Cardiac Troponin I antibody. Suitable for ELISA and reacts with Mouse, Rat, Human, Dog samples. Cited in 2 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human TNNI3.

View Alternative Names

TNNC1, TNNI3, Cardiac troponin I

1 Images
ELISA - Anti-Cardiac Troponin I antibody [M155] (AB10237)
  • ELISA

Unknown

ELISA - Anti-Cardiac Troponin I antibody [M155] (AB10237)

cTnI calibration curve. Monoclonal antibodies used for capture were biotinylated ab10237 (M155) at 200 ng/well. For detection Eu-labeled ab8289 (19C7) was used at 200 ng/well. The Antigens used included Human (grey triangle), Rat (cream circle), Canine (blue circle), and Mouse (blue square). This was then incubated for 20 min at 20°C.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M155

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Dog, Human

Applications

ELISA

applications

Immunogen

Native Full Length Protein corresponding to Human TNNI3.

P19429

Epitope

27-43 aa

Specificity

This antibody is reacting with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (In the presence of 5 mM EDTA). It is not affected by heparin, phosphorylation, oxidation and troponin complex formation. This antibody does not cross-react with skeletal muscle troponin I.

Reactivity data

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Product details

Concentration varies from lot to lot and can be provided on request.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purity tested by electrophoresis.
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
See full target information TNNI3

Publications (2)

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Analytical chemistry 94:2375-2382 PubMed35083913

2022

A Quantification of Target Protein Biomarkers in Complex Media by Faradaic Shotgun Tagging.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Sharafeldin,Felix Fleschhut,Timothy James,Jason J Davis

Journal of muscle research and cell motility 29:169-72 PubMed19172403

2009

Current techniques for the study of troponin I phosphorylation in human heart.

Applications

Unspecified application

Species

Unspecified reactive species

Clare E Gallon
View all publications

Product promise

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