Anti-Cardiac Troponin I antibody [RM1073]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Cardiac Troponin I with ab314912 at 1/5000 (0.102 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human skeletal muscle. The section was incubated with ab314912 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Cardiac Troponin I with ab314912 at 1/5000 (0.102 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cardiac muscle. The section was incubated with ab314912 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Cardiac Troponin I was immunoprecipitated from 0.35 mg Human heart tissue lysate with ab314912 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314912 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Human heart tissue lysate

Lane 2 : ab314912 IP in Human heart tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314912 in human heart tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] (ab314912) at 1/30 dilution

All lanes:

Human heart tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3s

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling Cardiac Troponin I with ab314912 at 1/50 (10.16 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control : confocal image showing no staining on mouse skeletal muscle (PMID : 22828728). The nuclear counterstain was DAPI (Blue). The section was incubated with ab314912 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Cardiac Troponin I with ab314912 at 1/5000 (0.102 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cardiac muscle. The section was incubated with ab314912 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Cardiac Troponin I with ab314912 at 1/5000 (0.102 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cardiac muscle. The section was incubated with ab314912 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cardiac muscle (fresh) tissue labeling Cardiac Troponin I with ab314912 at 1/50 (10.16 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on rat cardiac muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab314912 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cardiac muscle (fresh) tissue labeling Cardiac Troponin I with ab314912 at 1/50 (10.16 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on mouse cardiac muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab314912 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Cardiac Troponin I with ab314912 at 1/5000 (0.102 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat skeletal muscle. The section was incubated with ab314912 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Cardiac Troponin I with ab314912 at 1/5000 (0.102 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse skeletal muscle. The section was incubated with ab314912 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh) tissue labeling Cardiac Troponin I with ab314912 at 1/50 (10.16 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control : confocal image showing no staining on rat skeletal muscle (PMID : 22828728). The nuclear counterstain was DAPI (Blue). The section was incubated with ab314912 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Cardiac Troponin I was immunoprecipitated from 0.35 mg mouse heart tissue lysate with ab314912 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314912 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse heart tissue lysate

Lane 2 : ab314912 IP in Mouse heart tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314912 in mouse heart tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] (ab314912) at 1/30 dilution

All lanes:

Mouse heart tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 8s

• IP

Supplier Data

Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Cardiac Troponin I was immunoprecipitated from 0.35 mg Rat heart tissue lysate with ab314912 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314912 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat heart tissue lysate

Lane 2 : ab314912 IP in Rat heart tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314912 in rat heart tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] (ab314912) at 1/30 dilution

All lanes:

Rat heart tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : skeletal muscle (PMID : 1934363 and PMID : 8661099)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9836709).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Cardiac Troponin I antibody [RM1073] (ab314912) at 1/1000 dilution

Lane 1:

Human heart tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 26 kDa,36 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : skeletal muscle (PMID : 1934363 and PMID : 8661099)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Cardiac Troponin I antibody [RM1073] (ab314912) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Rat heart tissue lysate at 20 µg

Lane 4:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 26 kDa,36 kDa

false

Exposure time: 15s

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Cardiac Troponin I antibody [RM1073] (AB314912)

Indirect ELISA analysis of ab314912 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Human Cardiac Troponin I.

Antigen concentration : 1000 ng/ml