Rabbit Multiclonal Cardiac Troponin I antibody. Carrier free. Suitable for WB, IHC-P, IP, IHC-Fr, I-ELISA and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
Images
![Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (AB314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-frozen-sections-img423437.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (AB314913)")
![Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (AB314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-frozen-sections-img423436.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (AB314913)")
![Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (AB314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-frozen-sections-img423435.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (AB314913)")
![Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (AB314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-frozen-sections-img423434.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (AB314913)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (AB314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423417.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (AB314913)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IP | IHC-Fr | I-ELISA | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected | Expected | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Tested | Expected | Not recommended | Not recommended |
| Rat | Tested | Tested | Tested | Tested | Expected | Not recommended | Not recommended |
| Recombinant fragment - Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Alternative names
TNNC1, TNNI3, Cardiac troponin I
Recommended products
Rabbit Multiclonal Cardiac Troponin I antibody. Carrier free. Suitable for WB, IHC-P, IP, IHC-Fr, I-ELISA and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- RM1073
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab314913 is the carrier-free version of Anti-Cardiac Troponin I antibody [RM1073] ab314912.
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
16 product images
![Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-frozen-sections-img423437.jpg "Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh) tissue labeling Cardiac Troponin I with Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/50 (10.16 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control: confocal image showing no staining on rat skeletal muscle (PMID: 22828728). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Cardiac Troponin I antibody [RM1073] ab314912 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.<\p>
![Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-frozen-sections-img423436.jpg "Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cardiac muscle (fresh) tissue labeling Cardiac Troponin I with Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/50 (10.16 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on rat cardiac muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Cardiac Troponin I antibody [RM1073] ab314912 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.<\p>
![Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-frozen-sections-img423435.jpg "Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling Cardiac Troponin I with Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/50 (10.16 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control: confocal image showing no staining on mouse skeletal muscle (PMID: 22828728). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Cardiac Troponin I antibody [RM1073] ab314912 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.<\p>
![Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-frozen-sections-img423434.jpg "Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cardiac muscle (fresh) tissue labeling Cardiac Troponin I with Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/50 (10.16 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on mouse cardiac muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Cardiac Troponin I antibody [RM1073] ab314912 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.<\p>
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423417.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Cardiac Troponin I with Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/5000 (0.102 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat skeletal muscle. The section was incubated with Anti-Cardiac Troponin I antibody [RM1073] ab314912 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423416.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Cardiac Troponin I with Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/5000 (0.102 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse skeletal muscle. The section was incubated with Anti-Cardiac Troponin I antibody [RM1073] ab314912 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423415.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Cardiac Troponin I with Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/5000 (0.102 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human skeletal muscle. The section was incubated with Anti-Cardiac Troponin I antibody [RM1073] ab314912 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423414.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Cardiac Troponin I with Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/5000 (0.102 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cardiac muscle. The section was incubated with Anti-Cardiac Troponin I antibody [RM1073] ab314912 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423413.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Cardiac Troponin I with Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/5000 (0.102 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cardiac muscle. The section was incubated with Anti-Cardiac Troponin I antibody [RM1073] ab314912 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423412.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Cardiac Troponin I with Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/5000 (0.102 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cardiac muscle. The section was incubated with Anti-Cardiac Troponin I antibody [RM1073] ab314912 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunoprecipitation-img423382.jpg "Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Cardiac Troponin I was immunoprecipitated from 0.35 mg Rat heart tissue lysate with Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Rat heart tissue lysate
Lane 2: Anti-Cardiac Troponin I antibody [RM1073] ab314912 IP in Rat heart tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Cardiac Troponin I antibody [RM1073] ab314912 in rat heart tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] (Anti-Cardiac Troponin I antibody [RM1073] ab314912) at 1/30 dilution
All lanes: Rat heart tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 8s
![Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunoprecipitation-img423381.jpg "Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Cardiac Troponin I was immunoprecipitated from 0.35 mg mouse heart tissue lysate with Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Anti-Cardiac Troponin I antibody [RM1073] ab314912 IP in Mouse heart tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Cardiac Troponin I antibody [RM1073] ab314912 in mouse heart tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] (Anti-Cardiac Troponin I antibody [RM1073] ab314912) at 1/30 dilution
All lanes: Mouse heart tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 8s
![Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunoprecipitation-img423380.jpg "Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Cardiac Troponin I was immunoprecipitated from 0.35 mg Human heart tissue lysate with Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human heart tissue lysate
Lane 2: Anti-Cardiac Troponin I antibody [RM1073] ab314912 IP in Human heart tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Cardiac Troponin I antibody [RM1073] ab314912 in human heart tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] (Anti-Cardiac Troponin I antibody [RM1073] ab314912) at 1/30 dilution
All lanes: Human heart tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
![Western blot - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--western-blot-img423371.jpg "Western blot - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Western blot - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: skeletal muscle (PMID: 1934363 and PMID: 8661099)
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9836709).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Cardiac Troponin I antibody [RM1073] (Anti-Cardiac Troponin I antibody [RM1073] ab314912) at 1/1000 dilution
Lane 1: Human heart tissue lysate at 20 µg
Lane 2: Human skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 26 kDa, 36 kDa
Exposure time: 1s
![Western blot - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--western-blot-img423370.jpg "Western blot - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Western blot - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: skeletal muscle (PMID: 1934363 and PMID: 8661099)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Cardiac Troponin I antibody [RM1073] (Anti-Cardiac Troponin I antibody [RM1073] ab314912) at 1/1000 dilution
Lane 1: Mouse heart tissue lysate at 20 µg
Lane 2: Mouse skeletal muscle tissue lysate at 20 µg
Lane 3: Rat heart tissue lysate at 20 µg
Lane 4: Rat skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 26 kDa, 36 kDa
Exposure time: 15s
![Indirect ELISA - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--indirect-elisa-img423451.jpg "Indirect ELISA - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
Indirect ELISA - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)
This data was developed using Anti-Cardiac Troponin I antibody [RM1073] ab314912, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Cardiac Troponin I antibody [RM1073] ab314912 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Human Cardiac Troponin I.
Antigen concentration: 1000 ng/ml
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423416.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423415.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423414.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423413.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423412.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
![Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunoprecipitation-img423382.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
![Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunoprecipitation-img423381.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
![Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--immunoprecipitation-img423380.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
![Western blot - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--western-blot-img423371.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
![Western blot - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--western-blot-img423370.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
![Indirect ELISA - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-rm1073-bsa-and-azide-free-ab314913--indirect-elisa-img423451.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Cardiac Troponin I antibody [RM1073] - BSA and Azide free (ab314913)")
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