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AB251031

Anti-Cardiac Troponin I (phospho S22 + S23) antibody [EPR1059(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Cardiac Troponin I phospho S22 + S23 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

TNNC1, TNNI3, Cardiac troponin I

3 Images
Western blot - Anti-Cardiac Troponin I (phospho S22 + S23) antibody [EPR1059(2)] - BSA and Azide free (AB251031)
  • WB

Supplier Data

Western blot - Anti-Cardiac Troponin I (phospho S22 + S23) antibody [EPR1059(2)] - BSA and Azide free (AB251031)

This data was developed using ab190697, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cardiac Troponin I (phospho S22 + S23) antibody [EPR1059(2)] (<a href='/en-us/products/primary-antibodies/cardiac-troponin-i-phospho-s22-s23-antibody-epr10592-ab190697'>ab190697</a>) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

Lambda Phosphatase treated Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

false

Western blot - Anti-Cardiac Troponin I (phospho S22 + S23) antibody [EPR1059(2)] - BSA and Azide free (AB251031)
  • WB

Supplier Data

Western blot - Anti-Cardiac Troponin I (phospho S22 + S23) antibody [EPR1059(2)] - BSA and Azide free (AB251031)

This data was developed using ab190697, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cardiac Troponin I (phospho S22 + S23) antibody [EPR1059(2)] - BSA and Azide free (ab251031) at 1/10000 dilution

All lanes:

Human fetal heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

false

Western blot - Anti-Cardiac Troponin I (phospho S22 + S23) antibody [EPR1059(2)] - BSA and Azide free (AB251031)
  • WB

Supplier Data

Western blot - Anti-Cardiac Troponin I (phospho S22 + S23) antibody [EPR1059(2)] - BSA and Azide free (AB251031)

This data was developed using ab190697, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cardiac Troponin I (phospho S22 + S23) antibody [EPR1059(2)] (<a href='/en-us/products/primary-antibodies/cardiac-troponin-i-phospho-s22-s23-antibody-epr10592-ab190697'>ab190697</a>) at 1/2000 dilution

All lanes:

Rat heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

false

  • Unconjugated

    Anti-Cardiac Troponin I (phospho S22 + S23) antibody [EPR1059(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1059(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251031 is the carrier-free version of ab190697.

Serine 22 and Serine 23 of mature Cardiac Troponin I is equivalent to Serine 23 and Serine 24 of uncleaved Cardiac Troponin I.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
See full target information TNNI3 phospho S22 + S23
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com