AB58546

Anti-Cardiac Troponin I (phospho T143) antibody

Name: Anti-Cardiac Troponin I (phospho T143) antibody (ab58546) | Abcam
Brand: Abcam Limited
SKU: AB58546
Price: 545 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(8 Publications)

Rabbit Polyclonal Cardiac Troponin I phospho T143 antibody. Suitable for ELISA, WB and reacts with Mouse, Rat, Pig, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human TNNI3 phospho T143 aa 100-200.

View Alternative Names

TNNC1, TNNI3, Cardiac troponin I

1 Images

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Western blot - Anti-Cardiac Troponin I (phospho T143) antibody (AB58546)

All lanes:

Western blot - Anti-Cardiac Troponin I (phospho T143) antibody (ab58546) at 1/500 dilution

Lane 1:

Mouse heart extract without immunizing peptide

Lane 2:

Mouse heart extract with immunizing peptide

Predicted band size: 24 kDa

Observed band size: 30 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Pig, Rat

Applications

WB, ELISA

applications

Immunogen

Synthetic Peptide within Human TNNI3 phospho T143 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

P19429

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

See full target information TNNI3 phospho T143

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Experimental physiology 106:2235-2247 PubMed34605091

2021

Increased myofilament calcium sensitivity is associated with decreased cardiac troponin I phosphorylation in the diabetic rat heart.

Applications

Unspecified application

Species

Unspecified reactive species

Angela C Greenman,Gary M Diffee,Amelia S Power,Gerard T Wilkins,Olivia M S Gold,Jeffrey R Erickson,James C Baldi

Pathobiology : journal of immunopathology, molecul 86:274-284 PubMed31574524

2019

R5 HIV-1 gp120 Activates p38 MAPK to Induce Rat Cardiomyocyte Injury by the CCR5 Coreceptor.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Gao,Qiujuan Fang,Xi Zhang,Qin Xu,Hanhui Ye,Wenyan Guo,Jiao He,Yahong Chen,Ruixing Wang,Zhijuan Wu,Jing Yu

The Journal of general physiology 151:1070-1080 PubMed31366607

2019

Cardiac contractile dysfunction and protein kinase C-mediated myofilament phosphorylation in disease and aging.

Applications

Unspecified application

Species

Unspecified reactive species

Vani S Ravichandran,Himanshu J Patel,Francis D Pagani,Margaret V Westfall

American journal of physiology. Heart and circulat 315:H348-H356 PubMed29775410

2018

Apelin and APJ orchestrate complex tissue-specific control of cardiomyocyte hypertrophy and contractility in the hypertrophy-heart failure transition.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria N Parikh,Jing Liu,Ching Shang,Christopher Woods,Alex C Chang,Mingming Zhao,David N Charo,Zachary Grunwald,Yong Huang,Kinya Seo,Philip S Tsao,Daniel Bernstein,Pilar Ruiz-Lozano,Thomas Quertermous,Euan A Ashley

Journal of the American Heart Association 3:e000145 PubMed24772522

2014

Nuclear localization of a1A-adrenergic receptors is required for signaling in cardiac myocytes: an “inside-out” a1-AR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Steven C Wu,Erika F Dahl,Casey D Wright,Andrew L Cypher,Chastity L Healy,Timothy D O'Connell

Brazilian journal of medical and biological resear 46:128-37 PubMed23369981

2013

TNNI3K is a novel mediator of myofilament function and phosphorylates cardiac troponin I.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,Lin Wang,Li Song,Yan-Wan Zhang,Jue Ye,Rui-Xia Xu,Na Shi,Xian-Min Meng

Circulation research 109:1396-400 PubMed21998327

2011

Inhibition of PKCα/β with ruboxistaurin antagonizes heart failure in pigs after myocardial infarction injury.

Applications

Species

Pig

Dennis Ladage,Lisa Tilemann,Kiyotake Ishikawa,Robert N Correll,Yoshiaki Kawase,Steven R Houser,Jeffery D Molkentin,Roger J Hajjar

Journal of molecular and cellular cardiology 50:487-99 PubMed21156182

2010

Enhanced myofilament responsiveness upon β-adrenergic stimulation in post-infarct remodeled myocardium.

Applications

Species

Pig

Nicky M Boontje,Daphne Merkus,Ruud Zaremba,Amanda Versteilen,Monique C de Waard,Giulia Mearini,Vincent J de Beer,Lucie Carrier,Lori A Walker,Hans W M Niessen,Dobromir Dobrev,Ger J M Stienen,Dirk J Duncker,Jolanda van der Velden

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cardiac Troponin I (phospho T143) antibody

Western blot - Anti-Cardiac Troponin I (phospho T143) antibody (AB58546)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (8)

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