Anti-cTnT antibody 1C11 is a mouse monoclonal antibody that is used for cardiac troponin T IHC, ICC/IF, ELISA. Suitable for Human, Mouse, Rat and other species.
- Detects cardiac troponin T in Human cardiac muscle
- No cross-reactivity with skeletal troponin T, cTnI, or TnC
- Cited in over 280 publications
Images
![Immunocytochemistry - Anti-Cardiac Troponin T antibody [1C11] (AB8295), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-1c11-ab8295--immunocytochemistry-img54440.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-Cardiac Troponin T antibody [1C11] (AB8295)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (AB8295), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-1c11-ab8295--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71111.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (AB8295)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (AB8295), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-1c11-ab8295--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img20928.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (AB8295)")
![Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (AB8295), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-1c11-ab8295--sandwich-elisa-img415813.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (AB8295)")
Publications
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- Journal of cellular physiology 239:212-2262023Cadherin-5 facilitated the differentiation of human induced pluripotent stem cells into sinoatrial node-like pacemaker cells by regulating β-catenin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhang,Fengyuan Wang,Lin Yin,Yanhong Tang,Xi Wang,Congxin HuangPubMed 38149479
- Nature biomedical engineering 8:427-4422023Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeffrey Harding,Kristina Vintersten-Nagy,Huijuan Yang,Jean Kit Tang,Maria Shutova,Eric D Jong,Ju Hee Lee,Mohammad Massumi,Tatiana Oussenko,Zohreh Izadifar,Puzheng Zhang,Ian M Rogers,Michael B Wheeler,Stephen J Lye,Hoon-Ki Sung,ChengJin Li,Mohammad Izadifar,Andras NagyPubMed 37996616
- Journal of translational medicine 21:6902023Systematic in vivo candidate evaluation uncovers therapeutic targets for LMNA dilated cardiomyopathy and risk of Lamin A toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChia Yee Tan,Pui Shi Chan,Hansen Tan,Sung Wei Tan,Chang Jie Mick Lee,Jiong-Wei Wang,Shu Ye,Hendrikje Werner,Ying Jie Loh,Yin Loon Lee,Matthew Ackers-Johnson,Roger S Y Foo,Jianming JiangPubMed 37840136
- Proteome science 21:162023Proteomic analysis of neonatal mouse hearts shows PKA functions as a cardiomyocyte replication regulator.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLizhi Hu,Minglu Liang,Qin Jiang,Youming Jie,Long Chen,Fengxiao ZhangPubMed 37821903
- iScience 26:1080512023Long non-coding RNA regulates cardiomyocyte differentiation through H2A.Z-mediated LHX1 transcriptional activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Yu,Benzhi Cai,Yong Zhang,Juan Xu,Dongping Liu,Xiyang Zhang,Zhenbo Han,Yingying Ma,Lei Jiao,Manyu Gong,Xuewen Yang,Yanying Wang,Haodong Li,Lihua Sun,Yu Bian,Fan Yang,Lina Xuan,Haodi Wu,Baofeng Yang,Ying ZhangPubMed 37942009
- Communications biology 6:9692023Abnormal developmental trajectory and vulnerability to cardiac arrhythmias in tetralogy of Fallot with DiGeorge syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Ho Chan,Yin-Yu Lam,Nicodemus Wong,Lin Geng,Jilin Zhang,Virpi Ahola,Aman Zare,Ronald Adolphus Li,Fredrik Lanner,Wendy Keung,Yiu-Fai CheungPubMed 37740059
- Acta pharmaceutica Sinica. B 13:4875-48922023Sorting nexin 3 exacerbates doxorubicin-induced cardiomyopathy regulation of TFRC-dependent ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjing Yu,Yuehuai Hu,Zhiping Liu,Kaiteng Guo,Dinghu Ma,Mingxia Peng,Yuemei Wang,Jing Zhang,Xiaolei Zhang,Panxia Wang,Jiguo Zhang,Peiqing Liu,Jing LuPubMed 38045054
- Nature communications 14:49672023IRX2 regulates angiotensin II-induced cardiac fibrosis by transcriptionally activating EGR1 in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen-Guo Ma,Yu-Pei Yuan,Di Fan,Xin Zhang,Teng Teng,Peng Song,Chun-Yan Kong,Can Hu,Wen-Ying Wei,Qi-Zhu TangPubMed 37587150
- NPJ Regenerative medicine 8:392023LRRC10 regulates mammalian cardiomyocyte cell cycle during heart regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebecca J Salamon,Megan C McKeon,Jiyoung Bae,Xiaoya Zhang,Wyatt G Paltzer,Kayla N Wanless,Alyssa R Schuett,Dakota J Nuttall,Stephen A Nemr,Rupa Sridharan,Youngsook Lee,Timothy J Kamp,Ahmed I MahmoudPubMed 37507410
- Nature communications 14:43522023Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSine Yaganoglu,Konstantinos Kalyviotis,Christina Vagena-Pantoula,Dörthe Jülich,Benjamin M Gaub,Maaike Welling,Tatiana Lopes,Dariusz Lachowski,See Swee Tang,Armando Del Rio Hernandez,Victoria Salem,Daniel J Müller,Scott A Holley,Julien Vermot,Jian Shi,Nordine Helassa,Katalin Török,Periklis PantazisPubMed 37468521
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human TNNT2. Database link P45379
Abcam Recommends
Reactivity data
Select an application| IHC-P | ICC/IF | sELISA | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Expected | Tested |
| Dog | Expected | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Can be used as Capture or Detection antibody. |
Species Dog | Dilution info - | Notes Can be used as Capture or Detection antibody. |
Species Rat | Dilution info - | Notes Can be used as Capture or Detection antibody. |
Species Human | Dilution info - | Notes Can be used as Capture or Detection antibody. |
Associated Products
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6 products for Alternative Product
Target data
Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Alternative names
TnTc, Cardiac muscle troponin T, cTnT, TNNT2
Recommended products
Anti-cTnT antibody 1C11 is a mouse monoclonal antibody that is used for cardiac troponin T IHC, ICC/IF, ELISA. Suitable for Human, Mouse, Rat and other species.
- Detects cardiac troponin T in Human cardiac muscle
- No cross-reactivity with skeletal troponin T, cTnI, or TnC
- Cited in over 280 publications
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human TNNT2. Database link P45379
- Clone number
- 1C11
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, cTnI and TnC.
- Light chain type
- unknown
- Concentration
- Purification notes
Purified from TCS
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Anti-Cardiac Troponin T antibody [1C11] (ab8295) is a mouse monoclonal antibody and is validated for use in ICC/IF, IHC-P and sELISA.
Abcam's high quality validation processes ensure Anti-Cardiac Troponin T antibody [1C11] (ab8295) has high sensitivity and specificity.
Anti-Cardiac Troponin T antibody [1C11] (ab8295) has 14 independent reviews from customers.
Anti-Cardiac Troponin T antibody [1C11] (ab8295) specifically detects Cardiac Troponin T (UniProt ID: P45379; Molecular weight: 36kDa) and is sold in 200 µg selling sizes.
One of the top cited clones for this target with >350 citations. Cardiac Troponin T is a crucial key biomarker for myocardial infarction and heart failure, regulating muscle contraction and myofibril formation. Mutations in Cardiac Troponin T are linked to familial hypertrophic cardiomyopathy and dilated cardiomyopathy, with various isoforms expressed in the human heart. This antibody is a ket tool in acute coronary syndrome research, particularly in understanding Troponin T levels and heart attack diagnosis. It is widely used in studies of Cardiac Troponin T assays and cardiac-specific troponin
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cardiac Troponin T also known as cTnT or cardiac T plays an important role in muscle contraction. As a component of the troponin complex it binds to tropomyosin and facilitates the actin-myosin interaction in cardiac muscle fibers. cTnT has a molecular mass of approximately 39 kDa and is expressed mainly in cardiac tissue. Researchers identify it through various antibodies such as 1C11 and 1F11 ensuring precise detection and analysis.
- Biological function summary
Cardiac troponin T is integral to the regulation of cardiac muscle contraction. It forms part of the troponin complex which also includes troponin I and troponin C and is involved in calcium-mediated control of cardiac muscle contraction. The presence of cTnT influences the interaction of actin and myosin essential for heart muscle contraction and relaxation. This ensures efficient cardiac function and response to physiological demands.
- Pathways
Cardiac troponin T functions in the excitation-contraction coupling and the sarcomere organization pathways. Its role in these pathways establishes connections between electrical signals and mechanical contraction. It interacts closely with troponin I and troponin C facilitating the transmission of calcium's signal for cardiac muscle contraction. Proper function within these pathways is important for maintaining heart rhythm and muscle strength.
- Associated diseases and disorders
Cardiac troponin T serves as a biomarker for cardiovascular conditions. Elevated levels in the blood indicate myocardial infarction or acute coronary syndrome providing vital information for diagnosis and management. Alterations in the expression of cTnT can also be associated with cardiomyopathies where it might interact with other cardiac proteins during disease progression. The study of anti-cardiac troponin antibodies including anti T aids in understanding these conditions and developing therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
![Immunocytochemistry - Anti-Cardiac Troponin T antibody [1C11] (ab8295), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-1c11-ab8295--immunocytochemistry-img54440.jpg "Immunocytochemistry - Anti-Cardiac Troponin T antibody [1C11] (ab8295)")
Immunocytochemistry - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
Immunofluorescence staining of Cardiac Troponin T using ab8295 ioSkeletal Myocytes - Human iPSC derived cells ab277612, which were differentiated for 10 days post induction.
The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8295 at 5 µg/mL and Anti-beta Tubulin antibody - Loading Control ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
The antibody ab8295 gave comparable results using MeOH fixation (100%, 5 min).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-1c11-ab8295--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71111.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)")
Ye L et al. Decreased Yes-Associated Protein-1 (YAP1) Expression in Pediatric Hearts with Ventricular Septal Defects. PLoS One 10:e0139712 (2015).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
Paraffin-embedded Normal human Heart and iVSD heart tissue (were blocked using 10% FBS for 30 min) stained for Cardiac Troponin T (Red) using ab8295 at 1/200 dilution at room temperature for 2 hours. The slides were then incubated with Fluor® 555-conjugated anti-mouse (Abcam, Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) ab150107; 1:1,000 dilution). The nuclear counterstain was DAPI (Blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-1c11-ab8295--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img20928.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
ab8295 staining human normal heart. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.![Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (ab8295), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-1c11-ab8295--sandwich-elisa-img415813.jpg "Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (ab8295)")
Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
Calibration curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen.(dark blue) canine, (blue/grey) human, (grey) mouse, (black) rat. Monoclonal antibodies: capture, ab8295 [clone 1C11], 1 μg/well, detection ab1454 [clone 7E7], 200 ng/well. Assay time, 30 min at room temperature.
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