Rabbit Recombinant Monoclonal Cardiac Troponin T antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (AB223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img20708.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (AB223536)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (AB223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114085.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (AB223536)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (AB223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119481.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (AB223536)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (AB223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72395.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (AB223536)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (AB223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107814.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (AB223536)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Tested | Tested | Not recommended | Tested |
| Rat | Tested | Tested | Not recommended | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
6 products for Alternative Product
2 products for Alternative Version
Target data
Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Alternative names
TnTc, Cardiac muscle troponin T, cTnT, TNNT2
Recommended products
Rabbit Recombinant Monoclonal Cardiac Troponin T antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20266
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab223536 is the carrier-free version of Anti-Cardiac Troponin T antibody [EPR20266] ab209813.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img20708.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)
This IHC data was generated using the same anti-Cardiac Troponin T antibody clone [EPR20266] in a different buffer formulation (cat# Anti-Cardiac Troponin T antibody [EPR20266] ab209813).
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Cardiac Troponin T with Anti-Cardiac Troponin T antibody [EPR20266] ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on human cardiac muscle is observed [PMID: 9068591].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114085.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Cardiac Troponin T with Anti-Cardiac Troponin T antibody [EPR20266] ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative control: No staining on human skeletal muscle [PMID: 11375287].
Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cardiac Troponin T antibody [EPR20266] ab209813).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119481.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Cardiac Troponin T with Anti-Cardiac Troponin T antibody [EPR20266] ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on mouse cardiac muscle is observed [PMID: 9068591].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cardiac Troponin T antibody [EPR20266] ab209813).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72395.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Cardiac Troponin T with Anti-Cardiac Troponin T antibody [EPR20266] ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative control: No staining on mouse skeletal muscle [PMID: 11375287].
Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cardiac Troponin T antibody [EPR20266] ab209813).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107814.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Cardiac Troponin T with Anti-Cardiac Troponin T antibody [EPR20266] ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on rat cardiac muscle is observed [PMID: 9068591].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cardiac Troponin T antibody [EPR20266] ab209813).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18347.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Cardiac Troponin T with Anti-Cardiac Troponin T antibody [EPR20266] ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative control: No staining on rat skeletal muscle [PMID: 11375287].
Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cardiac Troponin T antibody [EPR20266] ab209813).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--flow-cytometry-intracellular-img414672.jpg "Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)")
Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)
This data was developed using Anti-Cardiac Troponin T antibody [EPR20266] ab209813, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HL-1 (Mouse atrial muscle cell) cells labelling Cardiac Troponin T with Anti-Cardiac Troponin T antibody [EPR20266] ab209813 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--flow-cytometry-intracellular-img415760.jpg "Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)")
Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)
This data was developed using Anti-Cardiac Troponin T antibody [EPR20266] ab209813, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized L6 (Rat skeletal muscle myoblast) cells labelling Cardiac Troponin T with Anti-Cardiac Troponin T antibody [EPR20266] ab209813 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18347.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)")
![Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--flow-cytometry-intracellular-img414672.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)")
![Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr20266-bsa-and-azide-free-ab223536--flow-cytometry-intracellular-img415760.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] - BSA and Azide free (ab223536)")
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