Rabbit Recombinant Monoclonal Cardiac Troponin T antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 16 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] (AB91605), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr3695-ab91605--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172275.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] (AB91605)")
![Western blot - Anti-Cardiac Troponin T antibody [EPR3695] (AB91605), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr3695-ab91605--western-blot-img167231.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cardiac Troponin T antibody [EPR3695] (AB91605)")
![Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] (AB91605), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr3695-ab91605--immunoprecipitation-img3797.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] (AB91605)")
Publications
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- Molecular therapy. Nucleic acids 34:1020602023Efficacy of exon-skipping therapy for DMD cardiomyopathy with mutations in actin binding domain 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaoko Shiba,Xiao Yang,Mitsuto Sato,Shin Kadota,Yota Suzuki,Masahiro Agata,Kohei Nagamine,Masaki Izumi,Yusuke Honda,Tomoya Koganehira,Hideki Kobayashi,Hajime Ichimura,Shinichiro Chuma,Junichi Nakai,Shugo Tohyama,Keiichi Fukuda,Daigo Miyazaki,Akinori Nakamura,Yuji ShibaPubMed 38028197
- NPJ Regenerative medicine 8:262023Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLynn Yap,Li Yen Chong,Clarissa Tan,Swarnaseetha Adusumalli,Millie Seow,Jing Guo,Zuhua Cai,Sze Jie Loo,Eric Lim,Ru San Tan,Elina Grishina,Poh Loong Soong,Narayan Lath,Lei Ye,Enrico Petretto,Karl TryggvasonPubMed 37236990
- Nucleic acids research 50:5313-53342022Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesW Samuel Fagg,Naiyou Liu,Ulrich Braunschweig,Karen Larissa Pereira de Castro,Xiaoting Chen,Frederick S Ditmars,Steven G Widen,John Paul Donohue,Katalin Modis,William K Russell,Jeffrey H Fair,Matthew T Weirauch,Benjamin J Blencowe,Mariano A Garcia-BlancoPubMed 35544276
- Frontiers in cardiovascular medicine 8:7688732021Cardiac Repair With Echocardiography-Guided Multiple Percutaneous Left Ventricular Intramyocardial Injection of hiPSC-CMs After Myocardial Infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXun Wu,Di Wang,Kele Qin,Chukwuemeka Daniel Iroegbu,Kun Xiang,Yuanjing Zhou,Qing Guan,Weijie Tang,Jun Peng,Jianjun Guo,Jinfu Yang,Chengming FanPubMed 34805322
- JCI insight 6:2021TT-10-loaded nanoparticles promote cardiomyocyte proliferation and cardiac repair in a mouse model of myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWangping Chen,Danielle Pretorius,Yang Zhou,Yuji Nakada,Jinfu Yang,Jianyi ZhangPubMed 34676829
- Stem cell research 53:1023652021Inhibition of EZH2 primes the cardiac gene activation via removal of epigenetic repression during human direct cardiac reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYawen Tang,Lianzhong Zhao,Xufen Yu,Jianyi Zhang,Li Qian,Jian Jin,Rui Lu,Yang ZhouPubMed 34087994
- Oncology reports 44:628-6362020TNNT2 as a potential biomarker for the progression and prognosis of colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Jing et. al.PubMed 32627044
- American journal of physiology. Heart and circulat 318:H801-H8152020DNA damage-free iPS cells exhibit potential to yield competent cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica M Miller et. al.PubMed 32057252
- Journal of visualized experiments : JoVE :2019Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng Zhao,Yawen Tang,Patrick J Ernst,Asher Kahn-Krell,Chengming Fan,Danielle Pretorius,Hanxi Zhu,Xi Lou,Lufang Zhou,Jianyi Zhang,Wuqiang ZhuPubMed 31355804
- Cells, tissues, organs 206:82-942019Hemodynamic Stimulation Using the Biomimetic Cardiac Tissue Model (BCTM) Enhances Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAaron J Rogers,Ramaswamy Kannappan,Hadil Abukhalifeh,Mohammed Ghazal,Jessica M Miller,Ayman El-Baz,Vladimir G Fast,Palaniappan SethuPubMed 30840966
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these. IHC-P validated in Human samples only. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Alternative names
TnTc, Cardiac muscle troponin T, cTnT, TNNT2
Recommended products
Rabbit Recombinant Monoclonal Cardiac Troponin T antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 16 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR3695
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr3695-ab91605--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172275.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labelling Cardiac Troponin T with purified ab91605 at 1/500 dilution (0.32 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Western blot - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr3695-ab91605--western-blot-img167231.jpg "Western blot - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)")
Western blot - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)
The molecular weight observed is consistent with what has been described in the literature (PMID: 15793653).
All lanes: Western blot - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605) at 1/1000 dilution
Lane 1: Human heart lysate at 20 µg
Lane 2: Human skeletal muscle lysate at 20 µg
Lane 3: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 39 kDa
![Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr3695-ab91605--immunoprecipitation-img3797.jpg "Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)")
Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)
Purified ab91605 at 1/50 dilution (2μg) immunoprecipitating Cardiac Troponin T in Human heart lysate.
Lane 1 (input): Human heart lysate 10μg
Lane 2 (+): ab91605 + Human heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab91605 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 39 kDaAll lanes: Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)
Predicted band size: 35 kDa
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