Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (AB247526)

This data was developed using ab91605, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Cardiac Troponin T with purified ab91605 at 1/500 dilution (0.32 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IP

Lab

Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (AB247526)

This data was developed using ab91605, the same antibody clone in a different buffer formulation.
Purified ab91605 at 1/50 dilution (2μg) immunoprecipitating Cardiac Troponin T in Human heart lysate.
Lane 1 (input) : Human heart lysate 10μg
Lane 2 (+) : ab91605 + Human heart lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab91605 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 39 kDa

All lanes:

Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] (<a href='/en-us/products/primary-antibodies/cardiac-troponin-t-antibody-epr3695-ab91605'>ab91605</a>)

Predicted band size: 35 kDa

false

• WB

Lab

Western blot - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (AB247526)

This data was developed using ab91605, the same antibody clone in a different buffer formulation.

The molecular weight observed is consistent with what has been described in the literature (PMID : 15793653).

All lanes:

Western blot - Anti-Cardiac Troponin T antibody [EPR3695] (<a href='/en-us/products/primary-antibodies/cardiac-troponin-t-antibody-epr3695-ab91605'>ab91605</a>) at 1/1000 dilution

Lane 1:

Human heart lysate at 20 µg

Lane 2:

Human skeletal muscle lysate at 20 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa

false