Rabbit Recombinant Monoclonal Cardiac Troponin T antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (AB247526), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr3695-bsa-and-azide-free-ab247526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172275.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (AB247526)")
![Western blot - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (AB247526), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr3695-bsa-and-azide-free-ab247526--western-blot-img167231.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (AB247526)")
![Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (AB247526), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr3695-bsa-and-azide-free-ab247526--immunoprecipitation-img3797.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (AB247526)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these. IHC-P validated in Human samples only. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
1 product for Alternative Product
Target data
Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Alternative names
TnTc, Cardiac muscle troponin T, cTnT, TNNT2
Recommended products
Rabbit Recombinant Monoclonal Cardiac Troponin T antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR3695
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247526 is the carrier-free version of Anti-Cardiac Troponin T antibody [EPR3695] ab91605.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr3695-bsa-and-azide-free-ab247526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172275.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)
This data was developed using Anti-Cardiac Troponin T antibody [EPR3695] ab91605, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Cardiac Troponin T with purified Anti-Cardiac Troponin T antibody [EPR3695] ab91605 at 1/500 dilution (0.32 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.![Western blot - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr3695-bsa-and-azide-free-ab247526--western-blot-img167231.jpg "Western blot - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)")
Western blot - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)
This data was developed using Anti-Cardiac Troponin T antibody [EPR3695] ab91605, the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in the literature (PMID: 15793653).
All lanes: Western blot - Anti-Cardiac Troponin T antibody [EPR3695] (Anti-Cardiac Troponin T antibody [EPR3695] ab91605) at 1/1000 dilution
Lane 1: Human heart lysate at 20 µg
Lane 2: Human skeletal muscle lysate at 20 µg
Lane 3: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 39 kDa
![Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-t-antibody-epr3695-bsa-and-azide-free-ab247526--immunoprecipitation-img3797.jpg "Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)")
Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free (ab247526)
This data was developed using Anti-Cardiac Troponin T antibody [EPR3695] ab91605, the same antibody clone in a different buffer formulation.
Purified Anti-Cardiac Troponin T antibody [EPR3695] ab91605 at 1/50 dilution (2μg) immunoprecipitating Cardiac Troponin T in Human heart lysate.
Lane 1 (input): Human heart lysate 10μg
Lane 2 (+): Anti-Cardiac Troponin T antibody [EPR3695] ab91605 + Human heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Cardiac Troponin T antibody [EPR3695] ab91605 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 39 kDaAll lanes: Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] (Anti-Cardiac Troponin T antibody [EPR3695] ab91605)
Predicted band size: 35 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com