AB239917

Anti-Cardiac Troponin T antibody [EPR3696] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Cardiac Troponin T antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

TnTc, Cardiac muscle troponin T, cTnT, TNNT2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3696] - BSA and Azide free (AB239917)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3696] - BSA and Azide free (AB239917)

This data was developed using ab92546, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Cardiac Troponin T with purified ab92546 at 1/1000 dilution (0.19 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

Lab

Western blot - Anti-Cardiac Troponin T antibody [EPR3696] - BSA and Azide free (AB239917)

This data was developed using ab92546 the same antibody clone in a different buffer formulation

The molecular weight observed is consistent with what has been described in the literature (PMID : 15793653).

All lanes:

Western blot - Anti-Cardiac Troponin T antibody [EPR3696] (<a href='/en-us/products/primary-antibodies/cardiac-troponin-t-antibody-epr3696-ab92546'>ab92546</a>) at 1/20000 dilution

All lanes:

Human heart lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa

false

Lab

Western blot - Anti-Cardiac Troponin T antibody [EPR3696] - BSA and Azide free (AB239917)

This data was developed using ab92546 the same antibody clone in a different buffer formulation.

The molecular weight observed is consistent with what has been described in the literature (PMID : 15793653).

All lanes:

Western blot - Anti-Cardiac Troponin T antibody [EPR3696] (<a href='/en-us/products/primary-antibodies/cardiac-troponin-t-antibody-epr3696-ab92546'>ab92546</a>) at 1/20000 dilution

Lane 1:

Rat heart lysate at 15 µg

Lane 2:

Mouse heart lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa

false

Unconjugated

Anti-Cardiac Troponin T antibody [EPR3696]
660 APC

APC Anti-Cardiac Troponin T antibody [EPR3696]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cardiac Troponin T antibody [EPR3696]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Cardiac Troponin T antibody [EPR3696]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Cardiac Troponin T antibody [EPR3696]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cardiac Troponin T antibody [EPR3696]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Cardiac Troponin T antibody [EPR3696]
578 PE

PE Anti-Cardiac Troponin T antibody [EPR3696]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3696

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab239917 is the carrier-free version of ab92546.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

See full target information TNNT2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 46: PubMed34528699

2021

Quantitative global proteome and phosphorylome analyses reveal potential biomarkers in kidney cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liwei Xu,Zeyi Lu,Shicheng Yu,Gonghui Li,Yuanlei Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cardiac Troponin T antibody [EPR3696] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3696] - BSA and Azide free (AB239917)

Western blot - Anti-Cardiac Troponin T antibody [EPR3696] - BSA and Azide free (AB239917)

Western blot - Anti-Cardiac Troponin T antibody [EPR3696] - BSA and Azide free (AB239917)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise