Rabbit Polyclonal CARM1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CARM1 aa 550-600.
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Publications
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- Molecular biology reports 50:7457-74692023Inhibition of CARM1 suppresses proliferation of multiple myeloma cells through activation of p53 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLan Yang,Le Ma,Qiang Gong,JiePing Chen,Qilin HuangPubMed 37477799
- Oncotarget 10:3709-37242019Functional interplay between YY1 and CARM1 promotes oral carcinogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmit K Behera et. al.PubMed 31217904
- The FEBS journal 285:1730-17442018p53 mediated regulation of coactivator associated arginine methyltransferase 1 (CARM1) expression is critical for suppression of adipogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmit K Behera,Aditya Bhattacharya,Madavan Vasudevan,Tapas K KunduPubMed 29575726
- Blood 120:3586-932012A tissue-specific chromatin loop activates the erythroid ankyrin-1 promoter.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshley O Yocum et. al.PubMed 22968456
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CARM1 aa 550-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q86X55
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Rat | Predicted |
| Chinese hamster | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Elephant | Predicted |
| Gorilla | Predicted |
| Orangutan | Predicted |
| Pig | Predicted |
| Rhesus monkey | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant | Dilution info - | Notes - |
Associated Products
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Target data
Function
Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability (PubMed:12237300, PubMed:16497732, PubMed:19405910). Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activation of transcription via chromatin remodeling (PubMed:12237300, PubMed:16497732, PubMed:19405910). During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription (By similarity). During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C (By similarity). During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B (By similarity). Acts as a coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue (By similarity). Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors (By similarity). Also seems to be involved in p53/TP53 transcriptional activation (By similarity). Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation (PubMed:15731352). Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs (By similarity). Acts as a transcriptional coactivator of ACACA/acetyl-CoA carboxylase by enriching H3R17 methylation at its promoter, thereby positively regulating fatty acid synthesis (By similarity). Independently of its methyltransferase activity, involved in replication fork progression: promotes PARP1 recruitment to replication forks, leading to poly-ADP-ribosylation of chromatin at replication forks and reduced fork speed (PubMed:33412112).
Alternative names
PRMT4, CARM1, Histone-arginine methyltransferase CARM1, Coactivator-associated arginine methyltransferase 1, Protein arginine N-methyltransferase 4
Recommended products
Rabbit Polyclonal CARM1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CARM1 aa 550-600.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CARM1 aa 550-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q86X55
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibody was affinity purified using an epitope specific to CARM1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CARM1 also known as PRMT4 is a protein with a mass of approximately 65 kDa. It belongs to the protein arginine methyltransferase (PRMT) family of enzymes. CARM1 modifies histones by methylating arginine residues which plays a role in regulating transcription. This protein expresses widely across diverse tissues but shows higher levels in the lung thymus and spleen. These expression patterns suggest its broad function in modulating gene expression within specialized tissues.
- Biological function summary
CARM1 serves as an important regulator of transcriptional activation. It can act independently or as part of a multiprotein complex to enhance transcription factor activity including steroid hormone receptors. In addition to histones CARM1 targets non-histone proteins like p300/CBP which affects their coactivator functions. It has a significant impact on gene expression by altering chromatin structure and accessibility thereby influencing cellular processes like differentiation and proliferation.
- Pathways
One can find CARM1 involved in pathways controlling gene expression and cell cycle regulation. The protein is particularly important in the estrogen receptor signaling pathway where it interacts with the estrogen receptor alpha (ERα) and modulates transcriptional activation of estrogen-responsive genes. It also plays a role in the p53 signaling pathway by enhancing p53-mediated transcriptional activation linking CARM1 to cell cycle arrest and apoptosis. Both pathways highlight its importance in cellular growth and homeostasis.
- Associated diseases and disorders
Abnormalities in CARM1 function connect to specific cancers including breast and prostate cancer. Its deregulation leads to altered methylation patterns that can drive cancer progression and metastasis. CARM1 influences the expression and activity of proteins such as cyclin D1 which affects cell cycle progression and tumor growth. Understanding the involvement of CARM1 in these disorders opens potential therapeutic avenues for targeting its aberrant pathways in cancer treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody (ab84370)
ab84370 at 1/100, staining human CARM1 in a section of human breast adenocarcinoma by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody (ab84370)
ab84370 at 1/500, staining human CARM1 in a section of human prostate adenocarcinoma by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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