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Rabbit Recombinant Monoclonal CARM1 antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Mouse, Human, Rat samples.

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Images

Western blot - Anti-CARM1 antibody [EPR26711-36] - BSA and Azide free (AB307092), expandable thumbnail
  • Western blot - Anti-CARM1 antibody [EPR26711-36] - BSA and Azide free (AB307092), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CARM1 antibody [EPR26711-36] - BSA and Azide free (AB307092), expandable thumbnail
  • Immunoprecipitation - Anti-CARM1 antibody [EPR26711-36] - BSA and Azide free (AB307092), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CARM1 antibody [EPR26711-36] - BSA and Azide free (AB307092), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPIPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Not recommended
Tested
Tested
Tested
Not recommended
Tested
Mouse
Not recommended
Tested
Tested
Tested
Not recommended
Tested
Rat
Not recommended
Expected
Expected
Expected
Not recommended
Tested

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Target data

Function

Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability (PubMed:12237300, PubMed:16497732, PubMed:19405910). Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activation of transcription via chromatin remodeling (PubMed:12237300, PubMed:16497732, PubMed:19405910). During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription (By similarity). During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C (By similarity). During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B (By similarity). Acts as a coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue (By similarity). Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors (By similarity). Also seems to be involved in p53/TP53 transcriptional activation (By similarity). Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation (PubMed:15731352). Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs (By similarity). Acts as a transcriptional coactivator of ACACA/acetyl-CoA carboxylase by enriching H3R17 methylation at its promoter, thereby positively regulating fatty acid synthesis (By similarity). Independently of its methyltransferase activity, involved in replication fork progression: promotes PARP1 recruitment to replication forks, leading to poly-ADP-ribosylation of chromatin at replication forks and reduced fork speed (PubMed:33412112).

Alternative names

PRMT4, CARM1, Histone-arginine methyltransferase CARM1, Coactivator-associated arginine methyltransferase 1, Protein arginine N-methyltransferase 4

Recommended products

Rabbit Recombinant Monoclonal CARM1 antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Mouse, Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26711-36
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CARM1 also known as PRMT4 is a protein with a mass of approximately 65 kDa. It belongs to the protein arginine methyltransferase (PRMT) family of enzymes. CARM1 modifies histones by methylating arginine residues which plays a role in regulating transcription. This protein expresses widely across diverse tissues but shows higher levels in the lung thymus and spleen. These expression patterns suggest its broad function in modulating gene expression within specialized tissues.

Biological function summary

CARM1 serves as an important regulator of transcriptional activation. It can act independently or as part of a multiprotein complex to enhance transcription factor activity including steroid hormone receptors. In addition to histones CARM1 targets non-histone proteins like p300/CBP which affects their coactivator functions. It has a significant impact on gene expression by altering chromatin structure and accessibility thereby influencing cellular processes like differentiation and proliferation.

Pathways

One can find CARM1 involved in pathways controlling gene expression and cell cycle regulation. The protein is particularly important in the estrogen receptor signaling pathway where it interacts with the estrogen receptor alpha (ERα) and modulates transcriptional activation of estrogen-responsive genes. It also plays a role in the p53 signaling pathway by enhancing p53-mediated transcriptional activation linking CARM1 to cell cycle arrest and apoptosis. Both pathways highlight its importance in cellular growth and homeostasis.

Associated diseases and disorders

Abnormalities in CARM1 function connect to specific cancers including breast and prostate cancer. Its deregulation leads to altered methylation patterns that can drive cancer progression and metastasis. CARM1 influences the expression and activity of proteins such as cyclin D1 which affects cell cycle progression and tumor growth. Understanding the involvement of CARM1 in these disorders opens potential therapeutic avenues for targeting its aberrant pathways in cancer treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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