JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB124730

Anti-Carma 1/CARD11 antibody [EPR2557]

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Recombinant Monoclonal Carma 1/CARD11 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CARMA1, CARD11, Caspase recruitment domain-containing protein 11, CARD-containing MAGUK protein 1, Carma 1

2 Images
Western blot - Anti-Carma 1/CARD11 antibody [EPR2557] (AB124730)
  • WB

Unknown

Western blot - Anti-Carma 1/CARD11 antibody [EPR2557] (AB124730)

All lanes:

Western blot - Anti-Carma 1/CARD11 antibody [EPR2557] (ab124730)

Lane 1:

Molt-4 cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 133 kDa

false

OI-RD Scanning - Anti-Carma 1/CARD11 antibody [EPR2557] (AB124730)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Carma 1/CARD11 antibody [EPR2557] (AB124730)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-Carma 1/CARD11 antibody [EPR2557] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2557

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB109310

Anti-Carma 1/CARD11 antibody [EPR3177]

RabMAb|Recombinant

Western blot - Anti-Carma 1/CARD11 antibody [EPR3177] (AB109310)

  • 0
  • 0
Primary Antibodies

AB115471

Anti-Carma 1/CARD11 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (AB115471)

  • 0
  • 0
Primary Antibodies

AB113409

Anti-Carma 1/CARD11 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (AB113409)

  • 0
  • 0
Primary Antibodies

AB91463

Anti-Carma 1/CARD11 antibody

Immunoprecipitation - Anti-Carma 1/CARD11 antibody (AB91463)

  • 0
  • 0
Primary Antibodies

AB264296

Anti-Carma 1/CARD11 antibody

Western blot - Anti-Carma 1/CARD11 antibody (AB264296)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Adapter protein that plays a key role in adaptive immune response by transducing the activation of NF-kappa-B downstream of T-cell receptor (TCR) and B-cell receptor (BCR) engagement (PubMed : 11278692, PubMed : 11356195, PubMed : 12356734). Transduces signals downstream TCR or BCR activation via the formation of a multiprotein complex together with BCL10 and MALT1 that induces NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways (PubMed : 11356195). Upon activation in response to TCR or BCR triggering, CARD11 homooligomerizes to form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10 and subsequent recruitment of MALT1 : this leads to I-kappa-B kinase (IKK) phosphorylation and degradation, and release of NF-kappa-B proteins for nuclear translocation (PubMed : 24074955). Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed : 17287217). Promotes linear ubiquitination of BCL10 by promoting the targeting of BCL10 to RNF31/HOIP (PubMed : 27777308). Stimulates the phosphorylation of BCL10 (PubMed : 11356195). Also activates the TORC1 signaling pathway (PubMed : 28628108).
See full target information CARD11
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 29:337-347 PubMed40051130

2025

Hyaluronan-mediated motility receptor-mediated aerobic glycolysis enhances stem-like properties and chemoresistance in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwen Yu,Yubo Shi,Xiaoqiong Bao,Xiangxiang Chen,Yangyang Ni,Jincong Wang,Hua Ye

International journal of molecular sciences 25: PubMed39408697

2024

Transcriptome Profiling Associated with Overexpression in Colorectal Cancer Implicates a Potential Role for Tumor Immune Microenvironment and Cancer Pathways Modulation via NF-κB.

Applications

Unspecified application

Species

Unspecified reactive species

Faisal Alhosani,Burcu Yener Ilce,Reem Sami Alhamidi,Poorna Manasa Bhamidimarri,Alaa Mohamed Hamad,Noura Alkhayyal,Axel Künstner,Cyrus Khandanpour,Hauke Busch,Basel Al-Ramadi,Kadria Sayed,Ali AlFazari,Riyad Bendardaf,Rifat Hamoudi

Experimental and therapeutic medicine 26:422 PubMed37602310

2023

KLF5‑mediated expression of CARD11 promotes the progression of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qiusen Li,Sheng Li,Zongxian Li,Hongyan Xu,Wenxian Zhang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com