Anti-Carma 1/CARD11 antibody [EPR29724-541]

• WB

Supplier Data

Western blot - Anti-Carma 1/CARD11 antibody [EPR29724-541] (AB324567)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Neuro-2a.

Negative control : brain (PMID : 11278692).

In lanes 3-7, the lysates were stored at -80℃ prior to Western Blotting. The bands beneath the target band (150 kDa) are likey to be degradation products. In lanes 1-2, To minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

All lanes:

Western blot - Anti-Carma 1/CARD11 antibody [EPR29724-541] (ab324567) at 1/1000 dilution

Lane 1:

A20 (mouse reticulum sarcoma B lymphocyte) fresh whole cell lysate at 80 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast) fresh whole cell lysate at 80 µg

Lane 3:

A20 (mouse reticulum sarcoma B lymphocyte) frozen whole cell lysate at 80 µg

Lane 4:

Neuro-2a (mouse neuroblastoma neuroblast) frozen whole cell lysate at 80 µg

Lane 5:

Mouse spleen frozen tissue lysate at 80 µg

Lane 6:

Mouse thymus frozen tissue lysate at 80 µg

Lane 7:

Mouse brain frozen tissue lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 150 kDa,15 kDa

false

Exposure time: 10s