AB242361

Anti-CARS antibody [CL2302]

Name: Anti-CARS antibody [CL2302] (ab242361) | Abcam
Brand: Abcam Limited
SKU: AB242361

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(1 Publication)

Mouse Monoclonal CARS antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CARS1 aa 500-700.

View Alternative Names

CARS, CARS1, Cysteinyl-tRNA synthetase, CysRS

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Paraffin-embedded human cerebellum tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Negative control :

Paraffin-embedded human skeletal muscle tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Paraffin-embedded human breast cancer tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Paraffin-embedded human pancreas tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Paraffin-embedded human fallopian tube tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-CARS antibody [CL2302] (AB242361)

All lanes:

Western blot - Anti-CARS antibody [CL2302] (ab242361) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cytoplasmic fraction

Lane 2:

HeLa membrane fraction

Lane 3:

HeLa nuclear fraction

Lane 4:

HeLa chromatin fraction

Lane 5:

HeLa cytoskeletal fraction

Predicted band size: 85 kDa

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Supplier Data

Western blot - Anti-CARS antibody [CL2302] (AB242361)

All lanes:

Western blot - Anti-CARS antibody [CL2302] (ab242361) at 1 µg/mL

All lanes:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 85 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2302

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CARS1 aa 500-700. The exact immunogen used to generate this antibody is proprietary information.

P49589

Epitope

Binds to an epitope located within the peptide sequence RPNQALLENIALYLT as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CARS protein also known as cysteinyl-tRNA synthetase is an enzyme responsible for attaching cysteine to its corresponding tRNA thereby playing a vital role in protein synthesis. The molecular mass of CARS is approximately 87 kDa and it is widely expressed in various human tissues including the liver and brain. As a part of the aminoacyl-tRNA synthetase (aaRS) family it ensures that the genetic code is accurately translated into functional proteins.

Biological function summary

Cysteinyl-tRNA synthetase acts as an important component in the translation machinery ensuring incorporation of cysteine into growing polypeptide chains during protein synthesis. It forms part of the multi-synthetase complex (MSC) which is important for cellular translation and efficiency. Beyond its primary synthetase activity CARS also participates in additional cellular processes such as response to oxidative stress by regulating cytosolic thioredoxin levels.

Pathways

Cysteinyl-tRNA synthetase plays a significant role within the translation pathway aiding in the biosynthesis of proteins by providing the essential building blocks. Another critical pathway involving CARS is the response to oxidative stress where it interacts with proteins like thioredoxin supporting cellular defense mechanisms against oxidative damage. These interactions highlight its importance in maintaining cellular homeostasis and function.

Alterations in CARS function have been associated with neurodegenerative disorders and certain types of cancer. In neurodegeneration abnormalities in cysteinyl-tRNA synthetase can lead to defective protein synthesis and oxidative stress imbalance. Additionally in specific cancers deregulation of protein synthesis pathways involving CARS and its interaction with proteins like thioredoxin contributes to tumor progression and resistance to chemotherapy. Understanding these connections aids in developing targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).

See full target information CARS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:902 PubMed40804742

2025

Cancer cell SPOCD1 promotes colorectal cancer liver metastasis by activating the CXCL12/CXCR4 signaling pathway in cancer-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Geng Peng,Lin Zhong,Lina Luo,Yongle Ju,Yan Lu,Lui Ng,Dominic Chi-Chung Foo,Fu Cheng,Luji Huang,Manzhao Ouyang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CARS antibody [CL2302]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Western blot - Anti-CARS antibody [CL2302] (AB242361)

Western blot - Anti-CARS antibody [CL2302] (AB242361)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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