AB126714

Anti-CARS antibody [EPR7121]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal CARS antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CARS, CARS1, Cysteinyl-tRNA synthetase, CysRS

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [EPR7121] (AB126714)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [EPR7121] (AB126714)

ab126714 at 1/50 dilution stains CARS in paraffin-embedded human breast carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CARS antibody [EPR7121] (AB126714)

All lanes:

Western blot - Anti-CARS antibody [EPR7121] (ab126714) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate

Lane 4:

BxPC-3 cell lysate

Lane 5:

MCF-7 cell lysate

Lane 6:

A549 cell lysate

Predicted band size: 85 kDa

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OI-RD Scanning - Anti-CARS antibody [EPR7121] (AB126714)

OI-RD Scanning

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OI-RD Scanning - Anti-CARS antibody [EPR7121] (AB126714)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-CARS antibody [EPR7121] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7121

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Antigen retrieval step is recommended.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CARS protein also known as cysteinyl-tRNA synthetase is an enzyme responsible for attaching cysteine to its corresponding tRNA thereby playing a vital role in protein synthesis. The molecular mass of CARS is approximately 87 kDa and it is widely expressed in various human tissues including the liver and brain. As a part of the aminoacyl-tRNA synthetase (aaRS) family it ensures that the genetic code is accurately translated into functional proteins.

Biological function summary

Cysteinyl-tRNA synthetase acts as an important component in the translation machinery ensuring incorporation of cysteine into growing polypeptide chains during protein synthesis. It forms part of the multi-synthetase complex (MSC) which is important for cellular translation and efficiency. Beyond its primary synthetase activity CARS also participates in additional cellular processes such as response to oxidative stress by regulating cytosolic thioredoxin levels.

Pathways

Cysteinyl-tRNA synthetase plays a significant role within the translation pathway aiding in the biosynthesis of proteins by providing the essential building blocks. Another critical pathway involving CARS is the response to oxidative stress where it interacts with proteins like thioredoxin supporting cellular defense mechanisms against oxidative damage. These interactions highlight its importance in maintaining cellular homeostasis and function.

Alterations in CARS function have been associated with neurodegenerative disorders and certain types of cancer. In neurodegeneration abnormalities in cysteinyl-tRNA synthetase can lead to defective protein synthesis and oxidative stress imbalance. Additionally in specific cancers deregulation of protein synthesis pathways involving CARS and its interaction with proteins like thioredoxin contributes to tumor progression and resistance to chemotherapy. Understanding these connections aids in developing targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).

See full target information CARS1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eady6386 PubMed40991712

2025

Site-specific adaptive nanovesicles for oral insulin delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Bozhang Xia,Fengfei Xu,Junge Chen,Shaobo Shan,Jiuheng Shen,Yuxuan Zhang,Jinjin Wang,Ziran Zhou,Weilun Sun,Yaru Jia,Ya-Li Zhang,Jie Zhong,Gang Zhang,Yichen Wan,Zhongbo Hu,Wenlin An,Yubo Fan,Massimo Bottini,Jinqiang Wang,Xing-Jie Liang

Nature cancer 3:471-485 PubMed35484422

2022

MYCN mediates cysteine addiction and sensitizes neuroblastoma to ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hamed Alborzinia,Andrés F Flórez,Sina Kreth,Lena M Brückner,Umut Yildiz,Moritz Gartlgruber,Dorett I Odoni,Gernot Poschet,Karolina Garbowicz,Chunxuan Shao,Corinna Klein,Jasmin Meier,Petra Zeisberger,Michal Nadler-Holly,Matthias Ziehm,Franziska Paul,Jürgen Burhenne,Emma Bell,Marjan Shaikhkarami,Roberto Würth,Sabine A Stainczyk,Elisa M Wecht,Jochen Kreth,Michael Büttner,Naveed Ishaque,Matthias Schlesner,Barbara Nicke,Carlo Stresemann,María Llamazares-Prada,Jan H Reiling,Matthias Fischer,Ido Amit,Matthias Selbach,Carl Herrmann,Stefan Wölfl,Kai-Oliver Henrich,Thomas Höfer,Andreas Trumpp,Frank Westermann

Frontiers in oncology 11:654076 PubMed34046350

2021

A Novel Ferroptosis Related Gene Signature for Prognosis Prediction in Patients With Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Nie,Dan Shan,Shun Li,Shuyuan Zhang,Xiaolin Zi,Fan Xing,Jiaqi Shi,Caiqi Liu,Tianjiao Wang,Xiaoyuan Sun,Qian Zhang,Meng Zhou,Shengnan Luo,Hongxue Meng,Yanqiao Zhang,Tongsen Zheng

Oncology reports 45: PubMed33846793

2021

CREB stimulates GPX4 transcription to inhibit ferroptosis in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixian Wang,Xiao Zhang,Xiaoting Tian,Yueyue Yang,Lifang Ma,Jiayi Wang,Yongchun Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CARS antibody [EPR7121]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [EPR7121] (AB126714)

Western blot - Anti-CARS antibody [EPR7121] (AB126714)

OI-RD Scanning - Anti-CARS antibody [EPR7121] (AB126714)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise