AB230522

Anti-CASC4 antibody - C-terminal

Name: Anti-CASC4 antibody - C-terminal (ab230522) | Abcam
Brand: Abcam Limited
SKU: AB230522

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(1 Publication)

Rabbit Polyclonal CASC4 antibody. C-terminal. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GOLM2 aa 300-400 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CASC4, UNQ2573/PRO6308, GOLM2, Protein GOLM2, Cancer susceptibility candidate gene 4 protein, Golgi membrane protein 2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASC4 antibody - C-terminal (AB230522)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASC4 antibody - C-terminal (AB230522)

Formalin-fixed, paraffin-embedded human prostate tissue stained for CASC4 with ab230522 at a 1 : 100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASC4 antibody - C-terminal (AB230522)

Formalin-fixed, paraffin-embedded human brain cortex tissue stained for CASC4 with ab230522 at a 1 : 100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-CASC4 antibody - C-terminal (AB230522)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CASC4 antibody - C-terminal (AB230522)

Intracellular Flow Cytometry analysis of Neuro-2a (Mouse neuroblastoma cell line) cells, labeling CASC4 with ab230522 at a 1/10 dilution (right side) compared to a negative control of (left side). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Supplier Data

Western blot - Anti-CASC4 antibody - C-terminal (AB230522)

All lanes:

Western blot - Anti-CASC4 antibody - C-terminal (ab230522) at 1/1000 dilution

All lanes:

Neuro-2a (Mouse neuroblastoma cell line) cell lysate at 35 µg

Predicted band size: 48 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human GOLM2 aa 300-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q6P4E1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CASC4 short for Cancer Susceptibility Candidate 4 is a protein involved in cell processes with a mass of approximately 87 kDa. It is expressed in various tissues with higher levels observed in specific cancer types. Its exact mechanical role in cells includes interactions and binding to other cellular elements influencing cell functions and stability. Researchers usually study this protein by its official name due to its relevance in cancer studies and its interaction potentials in cellular mechanisms.

Biological function summary

The protein plays a role in cellular growth and regulation. It is part of a larger complex that includes other signaling and structural proteins supporting its function in maintaining proper cellular environments. It may help ensure the cell cycle progresses smoothly influencing cell survival and replication. Its expression suggests it might help modulate responses under stress or during abnormal growth which can have implications for understanding certain cancer behaviors.

Pathways

CASC4 engages in cellular signaling and growth regulation pathways specifically those related to cancer biology. It is involved in pathways such as the PI3K/AKT pathway which is important for cell proliferation and survival. In these networks CASC4 connects with other proteins like AKT potentially influencing the pathway’s outcomes on cell fate decisions and tumor progression. This interaction suggests that CASC4 may have a role in modifying signaling outcomes depending on different cellular contexts.

Research indicates that CASC4 is associated with cancer progression and certain subtypes like breast and colorectal cancers. These associations hint that abnormal expression or functioning of CASC4 could contribute to oncogenic processes. It often interacts with proteins like PTEN in these disease contexts emphasizing its potential impact on tumor suppression or promotion. Understanding these interactions could prove beneficial in developing targeted therapies or diagnostic markers for these cancers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information GOLM2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 16: PubMed39766038

2025

CXCR1 Expression in MDA-PCa-2b Cell Upregulates ITM2A to Inhibit Tumor Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy O Adekoya,Nikia Smith,Parag Kothari,Monique A Dacanay,Yahui Li,Ricardo M Richardson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CASC4 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASC4 antibody - C-terminal (AB230522)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASC4 antibody - C-terminal (AB230522)

Flow Cytometry (Intracellular) - Anti-CASC4 antibody - C-terminal (AB230522)

Western blot - Anti-CASC4 antibody - C-terminal (AB230522)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise