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AB317147

Anti-CASC5 antibody [HL2158]

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Rabbit Recombinant Monoclonal CASC5/KNL1 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human KNL1.

View Alternative Names

CASC5, KIAA1570, KNL1, Outer kinetochore KNL1 complex subunit KNL1, ALL1-fused gene from chromosome 15q14 protein, Bub-linking kinetochore protein, Cancer susceptibility candidate gene 5 protein, Cancer/testis antigen 29, Kinetochore scaffold 1, Kinetochore-null protein 1, Protein CASC5, Protein D40/AF15q14, AF15q14, Blinkin, CT29

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CASC5 antibody [HL2158] (AB317147)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CASC5 antibody [HL2158] (AB317147)

ab317147 detects CASC5 protein at kinetochore by immunofluorescent analysis. Sample : HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : CASC5 stained by ab317147 diluted at 1 : 500. Red : alpha Tubulin a cytoskeleton marker stained by alpha Tubulin antibody [GT114] diluted at 1 : 1000. Blue : Fluoroshield with DAPI.

Western blot - Anti-CASC5 antibody [HL2158] (AB317147)
  • WB

Supplier Data

Western blot - Anti-CASC5 antibody [HL2158] (AB317147)

A431 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE and the membrane was blotted with ab317147 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-CASC5 antibody [HL2158] (ab317147) at 1/1000 dilution

Lane 1:

A431 whole cell extracts at 30 µg

Lane 2:

A431 nuclear extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2158

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human KNL1.

Q8NG31

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CASC5 also known as KNL1 or Blinkin is a large protein with a molecular mass of approximately 250 kDa. It is an essential component of the mitotic kinetochore complex which plays an important role in chromosome segregation during cell division. CASC5 is predominantly expressed in dividing cells and localizes to kinetochores during mitosis. The protein is critical for kinetochore-microtubule attachment ensuring the correct alignment and separation of chromosomes.
Biological function summary

CASC5/KNL1 is involved in maintaining genomic stability by facilitating proper chromosome segregation. It serves as a scaffold protein and forms part of the KMN network a core microtubule-binding complex at the kinetochore. This network also includes other key proteins such as NDC80 and Mis12 complex which coordinate the attachment of spindle microtubules to chromosomes. The presence of CASC5 in this complex is essential for its stability and function impacting the proper progression of the cell cycle.

Pathways

CASC5 participates in regulatory mechanisms associated with the cell cycle and mitotic checkpoints. It integrates into the spindle assembly checkpoint pathway important for preventing aneuploidy by ensuring that cells do not undergo mitosis until all chromosomes are correctly attached to the spindle apparatus. Within these pathways CASC5 interacts with proteins such as BUB1 and MAD1 which are checkpoint regulators that monitor spindle assembly and activate the mitotic checkpoint when errors are detected.

Improper function or expression of CASC5 has been linked to various cancers. Abnormal CASC5 activity can lead to chromosomal instability which is a hallmark of cancer progression and is often observed in aggressive tumors. CASC5 also connects to the protein Aurora B kinase in cancerous cells as Aurora B is involved in correcting kinetochore-microtubule attachment errors. Additionally CASC5 mutations have been associated with microcephaly a neurodevelopmental disorder where the protein's malfunction affects the proper division and proliferation of neural progenitor cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed : 15502821, PubMed : 17981135, PubMed : 18045986, PubMed : 19893618, PubMed : 21199919, PubMed : 22000412, PubMed : 22331848, PubMed : 27881301, PubMed : 30100357). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed : 18045986, PubMed : 19893618, PubMed : 27881301). The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed : 17981135, PubMed : 19893618, PubMed : 22000412, PubMed : 38459127, PubMed : 38459128). Required for kinetochore binding by a distinct subset of kMAPs (kinetochore-bound microtubule-associated proteins) and motors (PubMed : 19893618). Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore (PubMed : 18045986, PubMed : 27881301). Can bind either to microtubules or to the protein phosphatase 1 (PP1) catalytic subunits PPP1CA and PPP1CC (via overlapping binding sites), it has higher affinity for PP1 (PubMed : 30100357). Recruits MAD2L1 to the kinetochore and also directly links BUB1 and BUB1B to the kinetochore (PubMed : 17981135, PubMed : 19893618, PubMed : 22000412, PubMed : 22331848, PubMed : 25308863). In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I (By similarity). In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments (By similarity).
See full target information KNL1
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Product promise

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