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AB222055

Anti-CASC5/KNL1 antibody

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(3 Publications)

Rabbit Polyclonal CASC5/KNL1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KNL1 aa 1550-1700.

View Alternative Names

CASC5, KIAA1570, KNL1, Outer kinetochore KNL1 complex subunit KNL1, ALL1-fused gene from chromosome 15q14 protein, Bub-linking kinetochore protein, Cancer susceptibility candidate gene 5 protein, Cancer/testis antigen 29, Kinetochore scaffold 1, Kinetochore-null protein 1, Protein CASC5, Protein D40/AF15q14, AF15q14, Blinkin, CT29

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-CASC5/KNL1 antibody (AB222055)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CASC5/KNL1 antibody (AB222055)

PFA-fixed, Triton X-100 permeabilized A431(human bone osteosarcoma epithelial cell line) cells stained for CASC5/KNL1 (green) using ab222055 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KNL1 aa 1550-1700. The exact immunogen used to generate this antibody is proprietary information.

Q8NG31

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CASC5 also known as KNL1 or Blinkin is a large protein with a molecular mass of approximately 250 kDa. It is an essential component of the mitotic kinetochore complex which plays an important role in chromosome segregation during cell division. CASC5 is predominantly expressed in dividing cells and localizes to kinetochores during mitosis. The protein is critical for kinetochore-microtubule attachment ensuring the correct alignment and separation of chromosomes.
Biological function summary

CASC5/KNL1 is involved in maintaining genomic stability by facilitating proper chromosome segregation. It serves as a scaffold protein and forms part of the KMN network a core microtubule-binding complex at the kinetochore. This network also includes other key proteins such as NDC80 and Mis12 complex which coordinate the attachment of spindle microtubules to chromosomes. The presence of CASC5 in this complex is essential for its stability and function impacting the proper progression of the cell cycle.

Pathways

CASC5 participates in regulatory mechanisms associated with the cell cycle and mitotic checkpoints. It integrates into the spindle assembly checkpoint pathway important for preventing aneuploidy by ensuring that cells do not undergo mitosis until all chromosomes are correctly attached to the spindle apparatus. Within these pathways CASC5 interacts with proteins such as BUB1 and MAD1 which are checkpoint regulators that monitor spindle assembly and activate the mitotic checkpoint when errors are detected.

Improper function or expression of CASC5 has been linked to various cancers. Abnormal CASC5 activity can lead to chromosomal instability which is a hallmark of cancer progression and is often observed in aggressive tumors. CASC5 also connects to the protein Aurora B kinase in cancerous cells as Aurora B is involved in correcting kinetochore-microtubule attachment errors. Additionally CASC5 mutations have been associated with microcephaly a neurodevelopmental disorder where the protein's malfunction affects the proper division and proliferation of neural progenitor cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed : 15502821, PubMed : 17981135, PubMed : 18045986, PubMed : 19893618, PubMed : 21199919, PubMed : 22000412, PubMed : 22331848, PubMed : 27881301, PubMed : 30100357). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed : 18045986, PubMed : 19893618, PubMed : 27881301). The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed : 17981135, PubMed : 19893618, PubMed : 22000412, PubMed : 38459127, PubMed : 38459128). Required for kinetochore binding by a distinct subset of kMAPs (kinetochore-bound microtubule-associated proteins) and motors (PubMed : 19893618). Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore (PubMed : 18045986, PubMed : 27881301). Can bind either to microtubules or to the protein phosphatase 1 (PP1) catalytic subunits PPP1CA and PPP1CC (via overlapping binding sites), it has higher affinity for PP1 (PubMed : 30100357). Recruits MAD2L1 to the kinetochore and also directly links BUB1 and BUB1B to the kinetochore (PubMed : 17981135, PubMed : 19893618, PubMed : 22000412, PubMed : 22331848, PubMed : 25308863). In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I (By similarity). In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments (By similarity).
See full target information KNL1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 25:1909-1935 PubMed38424231

2024

CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments.

Applications

Unspecified application

Species

Unspecified reactive species

R Bhagya Lakshmi,Pinaki Nayak,Linoy Raz,Apurba Sarkar,Akshay Saroha,Pratibha Kumari,Vishnu M Nair,Delvin P Kombarakkaran,S Sajana,Sanusha M G,Sarit S Agasti,Raja Paul,Uri Ben-David,Tapas K Manna

The EMBO journal 42:e111587 PubMed37063065

2023

Targeted assembly of ectopic kinetochores to induce chromosome-specific segmental aneuploidies.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Tovini,Sarah C Johnson,Molly A Guscott,Alexander M Andersen,Diana Carolina Johanna Spierings,René Wardenaar,Floris Foijer,Sarah E McClelland

Journal of cell science 136: PubMed36744380

2023

Myo19 tethers mitochondria to endoplasmic reticulum-associated actin to promote mitochondrial fission.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen M Coscia,Cameron P Thompson,Qing Tang,Elana E Baltrusaitis,Joseph A Rhodenhiser,Omar A Quintero-Carmona,E Michael Ostap,Melike Lakadamyali,Erika L F Holzbaur
View all publications
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