AB166596

Anti-Casein antibody

Name: Anti-Casein antibody (ab166596) | Abcam
Brand: Abcam Limited
SKU: AB166596
Price: 430 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(14 Publications)

Anti-Casein antibody (ab166596) is a rabbit polyclonal antibody detecting Casein in Western Blot. Suitable for Cow.

View Alternative Names

Alpha-S1-casein, CSN1S1

1 Images

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Western blot - Anti-Casein antibody (AB166596)

All lanes:

Western blot - Anti-Casein antibody (ab166596) at 1/5000 dilution

Lane 1:

Bovine Milk - 2.5ng

Lane 2:

Bovine Milk - 10ng

Lane 3:

Bovine Milk - 25ng

Predicted band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Cow

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab166596 binds to the casein protein of both alpha and beta-chains. Theproduct may cross react with casein from other mammalian species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Casein antibody (ab166596) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Cow samples.

What is the molecular weight of Casein?
Anti-Casein (ab166596) specifically detects a band for Casein (UniProt: P47710) at a molecular weight of 22kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Important role in the capacity of milk to transport calcium phosphate.. Antioxidant peptide has 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity.

See full target information Alpha-S1-casein

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 36:102644 PubMed40896585

2025

RNAi delivery mediated by milk extracellular vesicles in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jessie Santoro,Silvia Nuzzo,Andrea Soricelli,Marco Salvatore,Anna Maria Grimaldi

Analytical and bioanalytical chemistry 417:2345-2359 PubMed40080124

2025

Isolation of bovine milk-derived extracellular vesicles via a capillary-channeled polymer (C-CP) fiber stationary phase.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Mata,Jerisa M Pimentel,Kun Huang,Alexis Stamatikos,R Kenneth Marcus

Journal of extracellular vesicles 13:e12462 PubMed38840457

2024

Milk-derived extracellular vesicles functionalized with anti-tumour necrosis factor-α nanobody and anti-microbial peptide alleviate ulcerative colitis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Renwei Jing,Leijie Zhang,Ruibin Li,Zhongqiu Yang,Jun Song,Qian Wang,Nan Cao,Gang Han,HaiFang Yin

Biomaterials research 27:124 PubMed38031117

2023

Post-insertion technique to introduce targeting moieties in milk exosomes for targeted drug delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Hochung Jang,Hyosuk Kim,Eun Hye Kim,Geonhee Han,Yeongji Jang,Yelee Kim,Jong Won Lee,Sang Chul Shin,Eunice EunKyeong Kim,Sun Hwa Kim,Yoosoo Yang

Journal of animal science 101: PubMed37782762

2023

Effects of L-arginine and arginine-arginine dipeptide on amino acids uptake and αS1-casein synthesis in bovine mammary epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Sun,Yue Cao,Yuanyuan Xing,Xiaojia Mu,Yihong Hao,Jing Yang,Xiaoyu Niu,Dabiao Li

Allergy 78:3193-3203 PubMed37497566

2023

Immunoglobulin G4 in eosinophilic esophagitis: Immune complex formation and correlation with disease activity.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan G Medernach,Rung-Chi Li,Xiao-Yu Zhao,Bocheng Yin,Emily A Noonan,Elaine F Etter,Shyam S Raghavan,Larry C Borish,Jeffrey M Wilson,Barrett H Barnes,Thomas A E Platts-Mills,Sarah E Ewald,Bryan G Sauer,Emily C McGowan

Immunology and cell biology 101:625-638 PubMed37157183

2023

The potential of dietary nanoparticles to enhance allergenicity of milk proteins: an in vitro investigation.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Xu,Wut Hmone Phue,Niladri Basu,Saji George

Journal of animal science 100: PubMed36222748

2022

Effects of glucose availability on αS1-casein synthesis in bovine mammary epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Sun,Zinan Li,Yuanyuan Xing,Xiaojia Mu,Yue Cao,Yihong Hao,Jing Yang,Dabiao Li

Nanotheranostics 5:488-498 PubMed34367882

2021

Novel Protocols for Scalable Production of High Quality Purified Small Extracellular Vesicles from Bovine Milk.

Applications

Unspecified application

Species

Unspecified reactive species

Spencer R Marsh,Kevin J Pridham,Jane Jourdan,Robert G Gourdie

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 166:61-74 PubMed34077790

2021

A focus on critical aspects of uptake and transport of milk-derived extracellular vesicles across the Caco-2 intestinal barrier model.

Applications

Unspecified application

Species

Unspecified reactive species

Josepha Roerig,Laura Schiller,Hermann Kalwa,Gerd Hause,Cica Vissiennon,Michael C Hacker,Christian Wölk,Michaela Schulz-Siegmund

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Casein antibody

Western blot - Anti-Casein antibody (AB166596)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (14)

Product promise