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Anti-Casein Kinase 1 alpha antibody [EPR19824] ab206652 is a rabbit monoclonal antibody that is used in Casein Kinase 1 alpha western blotting. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity confirmed with CSNK1A1 knockout cell line validation


Images

Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (AB206652), expandable thumbnail
  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (AB206652), expandable thumbnail
  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (AB206652), expandable thumbnail
  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (AB206652), expandable thumbnail
  • Immunoprecipitation - Anti-Casein Kinase 1 alpha antibody [EPR19824] (AB206652), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis (PubMed:11955436, PubMed:1409656, PubMed:18305108). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed:23902688).

Alternative names

Casein kinase I isoform alpha, CKI-alpha, CK1, CSNK1A1

Recommended products

Anti-Casein Kinase 1 alpha antibody [EPR19824] ab206652 is a rabbit monoclonal antibody that is used in Casein Kinase 1 alpha western blotting. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity confirmed with CSNK1A1 knockout cell line validation

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19824

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652), expandable thumbnail

    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

    Lane 1: Human fetal brain lysate at 20 µg

    Lane 2: Human fetal kidney lysate at 20 µg

    Lane 3: Human fetal spleen lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution

    Predicted band size: 39 kDa

    Observed band size: 36 kDa

    Exposure time: 3min

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652), expandable thumbnail

    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 20 µg

    Lane 2: Mouse kidney lysate at 20 µg

    Lane 3: Mouse spleen lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Predicted band size: 39 kDa

    Observed band size: 36 kDa

    Exposure time: 15s

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652), expandable thumbnail

    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The lysates were kindly provided by our collaborator Dr. Yi Rao, Peking University.

    All lanes: Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

    Lane 2: HEK-293T Casein Kinase 1 alpha KO whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Predicted band size: 39 kDa

    Observed band size: 36 kDa

    Exposure time: 1s

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206652).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The lysates were kindly provided by our collaborator Dr. Yi Rao, Peking University.

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652), expandable thumbnail

    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/10000 dilution

    Lane 1: Rat brain lysate at 20 µg

    Lane 2: Rat kidney lysate at 20 µg

    Lane 3: Rat spleen lysate at 20 µg

    Predicted band size: 30 kDa, 39 kDa

    Observed band size: 36 kDa

    Exposure time: 5s

  • Immunoprecipitation - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652), expandable thumbnail

    Immunoprecipitation - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)

    Casein Kinase 1 alpha was immunoprecipitated from 0.35 mg of Human skin lysate with ab206652 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206652 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human skin lysate, 10μg (Input).

    Lane 2: ab206652 IP in Human skin lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab206652 in Human skin lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)

    Predicted band size: 39 kDa

    Observed band size: 36 kDa

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652), expandable thumbnail

    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2: 15 seconds; Lane 3: 3 min; Lane 4/5/6: 1 second.

    All lanes: Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

    Lane 1: Human skin lysate at 20 µg

    Lane 2: Mouse skin lysate at 20 µg

    Lane 3: Rat skin lysate at 20 µg

    Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 5: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 6: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Predicted band size: 39 kDa

    Observed band size: 36 kDa

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652), expandable thumbnail

    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)

    ab206652 was shown to react with CSNK1A1 in wild-type HCT116 cells in Western blot with loss of signal observed in a CSNK1A1 siRNA knockdown cell line. Cell lysates from wild-type HCT116 transfected with either scrambled siRNA or CSNK1A1 siRNA were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab206652 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

    This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

    Lane 1: Wild-type HCT116 transfected with scrambled siRNA control lysate at 50 µg

    Lane 2: HCT116 transfected with siRNA specifically targeting CSNK1A1 cell lysate at 50 µg

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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