Anti-Casein Kinase 1 alpha antibody [EPR19824]

• IP

Supplier Data

Immunoprecipitation - Anti-Casein Kinase 1 alpha antibody [EPR19824] (AB206652)

Casein Kinase 1 alpha was immunoprecipitated from 0.35 mg of Human skin lysate with ab206652 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206652 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human skin lysate, 10μg (Input).

Lane 2 : ab206652 IP in Human skin lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab206652 in Human skin lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652)

Predicted band size: 39 kDa

Observed band size: 36 kDa

false

• WB

Supplier Data

Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (AB206652)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Lane 3:

Human fetal spleen lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Predicted band size: 39 kDa

Observed band size: 36 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (AB206652)

Blocking/Dilution buffer : 5% NFDM/TBST.

The lysates were kindly provided by our collaborator Dr. Yi Rao, Peking University.

All lanes:

Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

HEK-293T Casein Kinase 1 alpha KO whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 39 kDa

Observed band size: 36 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (AB206652)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1/2 : 15 seconds; Lane 3 : 3 min; Lane 4/5/6 : 1 second.

All lanes:

Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

Lane 1:

Human skin lysate at 20 µg

Lane 2:

Mouse skin lysate at 20 µg

Lane 3:

Rat skin lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 5:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 39 kDa

Observed band size: 36 kDa

false

• WB

Lab

Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (AB206652)

ab206652 was shown to react with CSNK1A1 in wild-type HCT116 cells in Western blot with loss of signal observed in a CSNK1A1 siRNA knockdown cell line. Cell lysates from wild-type HCT116 transfected with either scrambled siRNA or CSNK1A1 siRNA were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab206652 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

Lane 1:

Wild-type HCT116 transfected with scrambled siRNA control lysate at 50 µg

Lane 2:

HCT116 transfected with siRNA specifically targeting CSNK1A1 cell lysate at 50 µg

false

• WB

Supplier Data

Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (AB206652)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Mouse kidney lysate at 20 µg

Lane 3:

Mouse spleen lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 39 kDa

Observed band size: 36 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (AB206652)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/10000 dilution

Lane 1:

Rat brain lysate at 20 µg

Lane 2:

Rat kidney lysate at 20 µg

Lane 3:

Rat spleen lysate at 20 µg

Predicted band size: 30 kDa,39 kDa

Observed band size: 36 kDa

false

Exposure time: 5s