Anti-Casein Kinase 1 alpha antibody [RP23040231]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 1 alpha Antibody [RP23040231] (AB313493)

Immunofluorescent analysis of HCT 116 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous CSNK1A1 with ab313493 at 1/100 dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Nuclei (blue) were stained using DAPI, and Rhodamine Phalloidin at 1/300 dilution was used for cytoskeletal F-actin (red) staining. Detection and localization of CSNK1A1 (green) in the nucleus and cytoplasm can be clearly observed in interphase cells (a-d). When cell cycle progresses to prophase (e-h) CSNK1A1 can be visualized detaching from chromatin and further progression to metaphase (i-l) and anphase and telophase (m-t) results in complete detachment of CSNK1A1 from chromatin and translocation to cytoplasm. The images were captured at 60X magnification.