Rabbit Monoclonal Casein Kinase 1 alpha antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Casein kinase I isoform alpha.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 1 alpha Antibody [RP23040231] (AB313493), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-alpha-antibody-rp23040231-ab313493--immunocytochemistry-immunofluorescence-img418019.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 1 alpha Antibody [RP23040231] (AB313493)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human Casein kinase I isoform alpha. Database link P48729
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Target data
Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (PubMed:11955436, PubMed:1409656, PubMed:18305108, PubMed:23902688). It can phosphorylate a large number of proteins (PubMed:11955436, PubMed:1409656, PubMed:18305108, PubMed:23902688). Participates in Wnt signaling (PubMed:11955436). Phosphorylates CTNNB1 at 'Ser-45' (PubMed:11955436). May phosphorylate PER1 and PER2 (By similarity). May play a role in segregating chromosomes during mitosis (PubMed:1409656). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed:23902688). Acts as a positive regulator of mTORC1 and mTORC2 signaling in response to nutrients by mediating phosphorylation of DEPTOR inhibitor (PubMed:22017875, PubMed:22017877). Acts as an inhibitor of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3 (By similarity).
Alternative names
Casein kinase I isoform alpha, CKI-alpha, CK1, CSNK1A1
Recommended products
Rabbit Monoclonal Casein Kinase 1 alpha antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Casein kinase I isoform alpha.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human Casein kinase I isoform alpha. Database link P48729
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 1 alpha Antibody [RP23040231] (ab313493), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-alpha-antibody-rp23040231-ab313493--immunocytochemistry-immunofluorescence-img418019.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 1 alpha Antibody [RP23040231] (ab313493)")
Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 1 alpha Antibody [RP23040231] (ab313493)
Immunofluorescent analysis of HCT 116 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous CSNK1A1 with ab313493 at 1/100 dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Nuclei (blue) were stained using DAPI, and Rhodamine Phalloidin at 1/300 dilution was used for cytoskeletal F-actin (red) staining. Detection and localization of CSNK1A1 (green) in the nucleus and cytoplasm can be clearly observed in interphase cells (a-d). When cell cycle progresses to prophase (e-h) CSNK1A1 can be visualized detaching from chromatin and further progression to metaphase (i-l) and anphase and telophase (m-t) results in complete detachment of CSNK1A1 from chromatin and translocation to cytoplasm. The images were captured at 60X magnification.
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