AB63559

Anti-Casein Kinase 1 alpha (phospho T321) antibody

Name: Anti-Casein Kinase 1 alpha (phospho T321) antibody (ab63559) | Abcam
Brand: Abcam Limited
SKU: AB63559

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(1 Publication)

Rabbit Polyclonal Casein Kinase 1 alpha phospho T321 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CSNK1A1 pT321 aa 300-350.

View Alternative Names

Casein kinase I isoform alpha, CKI-alpha, CK1, CSNK1A1

1 Images

Unknown

Western blot - Anti-Casein Kinase 1 alpha (phospho T321) antibody (AB63559)

All lanes:

Western blot - Anti-Casein Kinase 1 alpha (phospho T321) antibody (ab63559) at 1/500 dilution

Lane 1:

extracts from HuvEc cells at 30 µg

Lane 2:

extracts from HuvEc cells at 30 µg with immunizing phosphopeptide

Predicted band size: 39 kDa

Observed band size: 39 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human CSNK1A1 pT321 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

P48729

Specificity

Detects Casein Kinase I alpha only when phosphorylated at threonine 321.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (PubMed : 11955436, PubMed : 1409656, PubMed : 18305108, PubMed : 23902688). It can phosphorylate a large number of proteins (PubMed : 11955436, PubMed : 1409656, PubMed : 18305108, PubMed : 23902688). Participates in Wnt signaling (PubMed : 11955436). Phosphorylates CTNNB1 at 'Ser-45' (PubMed : 11955436). May phosphorylate PER1 and PER2 (By similarity). May play a role in segregating chromosomes during mitosis (PubMed : 1409656). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed : 23902688). Acts as a positive regulator of mTORC1 and mTORC2 signaling in response to nutrients by mediating phosphorylation of DEPTOR inhibitor (PubMed : 22017875, PubMed : 22017877). Acts as an inhibitor of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3 (By similarity).

See full target information CSNK1A1 pT321

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer letters 340:82-7 PubMed23850566

2013

Telomerase activation as a repair response to radiation-induced DNA damage in Y79 retinoblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Masaharu Akiyama,Kohji Ozaki,Takeshi Kawano,Osamu Yamada,Kiyotaka Kawauchi,Hiroyuki Ida,Hisashi Yamada

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Casein Kinase 1 alpha (phospho T321) antibody

Western blot - Anti-Casein Kinase 1 alpha (phospho T321) antibody (AB63559)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise