Anti-Casein Kinase 1 delta/CSNK1D antibody
Be the first to review this product! Submit a review
|
(2 Publications)
Rabbit Polyclonal Casein Kinase 1 delta/CSNK1D antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CSNK1D aa 150-400.
View Alternative Names
HCKID, CSNK1D, Casein kinase I isoform delta, CKI-delta, CKId, Tau-protein kinase CSNK1D
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 1 delta/CSNK1D antibody (AB236601)
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Casein Kinase 1 delta/CSNK1D using ab236601 at a dilution of 1/333 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 1 delta/CSNK1D antibody (AB236601)
Paraffin-embedded human liver cancer tissue stained for Casein Kinase 1 delta/CSNK1D with ab236601 at a 1/1000 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
- WB
Supplier Data
Western blot - Anti-Casein Kinase 1 delta/CSNK1D antibody (AB236601)
All lanes:
Western blot - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab236601) at 1/1000 dilution
All lanes:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 1 delta/CSNK1D antibody (AB236601)
Paraffin-embedded human skeletal muscle tissue stained for Casein Kinase 1 delta/CSNK1D with ab236601 at a 1/1000 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 1 delta/CSNK1D antibody (AB236601)
Paraffin-embedded human lymph node tissue stained for Casein Kinase 1 delta/CSNK1D with ab236601 at a 1/1000 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
- IP
Supplier Data
Immunoprecipitation - Anti-Casein Kinase 1 delta/CSNK1D antibody (AB236601)
Casein Kinase 1 delta/CSNK1D was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate using 6 μg of ab236601.
Lane 1 : Rabbit control IgG (1 μg) in HeLa whole cell lysate.
Lane 2 : ab236601 (6 μg) in HeLa whole cell lysate.
Lane 3 : HeLa whole cell lysate (10 μg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at a 1/2000 dilution.
All lanes:
Immunoprecipitation - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab236601)
Predicted band size: 47 kDa
false
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (2)
Recent publications for all applications. Explore the full list and refine your search
Acta neuropathologica 147:104 PubMed38896345
2024
Applications
Unspecified application
Species
Unspecified reactive species
Cellular and molecular life sciences : CMLS 80:156 PubMed37208565
2023
Applications
Unspecified application
Species
Unspecified reactive species
Alternative Products
Complementary Products
Primary Antibodies
AB9485
Anti-GAPDH antibody - Loading Control
BOND RX™ Validated|Lab Essentials
- 5
- 88
Primary Antibodies
AB32072
Anti-c-Myc antibody [Y69] - ChIP Grade
BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)](https://content.abcam.com/products/images/c-myc-antibody-y69-chip-grade-ab32072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80839.jpg)
- 4
- 56
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com