Rabbit Polyclonal Casein Kinase 1 gamma 2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human CSNK1G2 aa 300 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CSNK1G2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P78368
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation (PubMed:15077195, PubMed:15917222, PubMed:18794808, PubMed:19005213). Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate (By similarity). Involved in regulation of reactive oxygen species (ROS) levels (PubMed:37099597).
Alternative names
CK1G2, CSNK1G2, Casein kinase I isoform gamma-2, CKI-gamma 2
Recommended products
Rabbit Polyclonal Casein Kinase 1 gamma 2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human CSNK1G2 aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CSNK1G2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P78368
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody (ab230045)
Casein Kinase 1 gamma 2 was immunoprecipitated from 500 μg HeLa epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 μg of ab230045. Western blot was performed from the immunoprecipitate using ab230045 at 1/1000 dilution.
Lane 1: 1 μg of Rabbit monoclonal IgG instead of ab230045 in HeLa whole cell lysate.
Lane 2: ab230045 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 μg (input).For western blotting, an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used the secondary antibody (1/50,000).
All lanes: Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody (ab230045)
Predicted band size: 47 kDa
Western blot - Anti-Casein Kinase 1 gamma 2 antibody (ab230045)
All lanes: Western blot - Anti-Casein Kinase 1 gamma 2 antibody (ab230045) at 1/1000 dilution
Lane 1: Mouse gonadal tissue lysate
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
SecondaryAll lanes: Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 1 gamma 2 antibody (ab230045)
Paraffin-embedded human breast cancer tissue stained for Casein Kinase 1 gamma 2 using ab230045 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 1 gamma 2 antibody (ab230045)
Paraffin-embedded human testis tissue stained for Casien Kinase 1 gamma 2 using ab230045 at 1/100 dilution in immunohistochemical analysis.
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