AB64829

Anti-Casein Kinase 1 gamma 2 antibody

Name: Anti-Casein Kinase 1 gamma 2 antibody (ab64829) | Abcam
Brand: Abcam Limited
SKU: AB64829
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal Casein Kinase 1 gamma 2 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human CSNK1G2.

View Alternative Names

CK1G2, CSNK1G2, Casein kinase I isoform gamma-2, CKI-gamma 2

2 Images

Unknown

Western blot - Anti-Casein Kinase 1 gamma 2 antibody (AB64829)

All lanes:

Western blot - Anti-Casein Kinase 1 gamma 2 antibody (ab64829) at 1/500 dilution

Lane 1:

HeLa cell extract at 30 µg

Lane 2:

293 cell extract at 30 µg

Lane 3:

293 cell extract at 30 µg with immunising peptide

Predicted band size: 47 kDa

Observed band size: 25 kDa,34 kDa,47 kDa

false

AbReview32952****

Western blot - Anti-Casein Kinase 1 gamma 2 antibody (AB64829)

Lanes 1 - 2:

Anti-Casein Kinase 1 gamma 2 antibody (<a href='/en-us/products/unavailable/casein-kinase-1-gamma-2-antibody-ab102554'>ab102554</a>)

Lanes 3 - 4:

Anti-Casein Kinase 1 gamma 2 antibody (<a href='/en-us/products/unavailable/casein-kinase-1-gamma-2-antibody-ab70862'>ab70862</a>)

Lanes 5 - 6:

Western blot - Anti-Casein Kinase 1 gamma 2 antibody (ab64829) at 1/500 dilution

Lanes 1, 3 and 5:

U2OS cells expressing FLAG-tagged CK1G2 plasmid

Lane 2:

U2OS cells expressing FLAG tag only plasmid

Lanes 4 and 6:

U2OS cells expressing FLAG only

Secondary

All lanes:

HRP-conjugated Goat anti-rabbit polyclonal at 1/1000 dilution

Predicted band size: 47 kDa

true

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human CSNK1G2. The exact immunogen used to generate this antibody is proprietary information.

P78368

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation (PubMed : 15077195, PubMed : 15917222, PubMed : 18794808, PubMed : 19005213). Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate (By similarity). Involved in regulation of reactive oxygen species (ROS) levels (PubMed : 37099597).

See full target information CSNK1G2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:101986 PubMed35487243

2022

Protein proximity networks and functional evaluation of the casein kinase 1 gamma family reveal unique roles for CK1γ3 in WNT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Megan J Agajanian,Frances M Potjewyd,Brittany M Bowman,Smaranda Solomon,Kyle M LaPak,Dhaval P Bhatt,Jeffery L Smith,Dennis Goldfarb,Alison D Axtman,Michael B Major

Molecular medicine reports 17:7559-7566 PubMed29620268

2018

Induction of Huh‑7 cell apoptosis by HCV core proteins via CK1α‑p53‑Bid signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Shen,Chunyang Li,Mingjia Dai,Xuebing Yan

Proceedings of the National Academy of Sciences of 114:7148-7153 PubMed28533369

2017

Phosphorylated Presenilin 1 decreases β-amyloid by facilitating autophagosome-lysosome fusion.

Applications

Species

Mouse

Victor Bustos,Maria V Pulina,Ashley Bispo,Alison Lam,Marc Flajolet,Fred S Gorelick,Paul Greengard

Oncogene 36:429-438 PubMed27321178

2016

Activation of CK1ɛ by PP2A/PR61ɛ is required for the initiation of Wnt signaling.

Applications

Unspecified application

Species

Human

M Vinyoles,B Del Valle-Pérez,J Curto,M Padilla,A Villarroel,J Yang,A G de Herreros,M Duñach

Molecular and cellular biology 31:2877-88 PubMed21606194

2011

Coordinated action of CK1 isoforms in canonical Wnt signaling.

Applications

Species

Human

Beatriz Del Valle-Pérez,Oriol Arqués,Meritxell Vinyoles,Antonio García de Herreros,Mireia Duñach

Journal of cell science 123:2621-31 PubMed20940130

2010

A p120-catenin-CK1epsilon complex regulates Wnt signaling.

Applications

Species

Human

David Casagolda,Beatriz Del Valle-Pérez,Gabriela Valls,Ero Lugilde,Meritxell Vinyoles,Juan Casado-Vela,Guiomar Solanas,Eduard Batlle,Albert B Reynolds,José Ignacio Casal,Antonio García de Herreros,Mireia Duñach

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Casein Kinase 1 gamma 2 antibody

Western blot - Anti-Casein Kinase 1 gamma 2 antibody (AB64829)

Western blot - Anti-Casein Kinase 1 gamma 2 antibody (AB64829)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (6)

Product promise