Rabbit Recombinant Monoclonal Casein Kinase 1 gamma 2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human, Transfected cell lysate - Human samples. Cited in 1 publication.
Images
![Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-ab238121--western-blot-img7791.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)")
![Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-ab238121--flow-cytometry-intracellular-img80365.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)")
![Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-ab238121--immunoprecipitation-img5959.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)")
![Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-ab238121--western-blot-img55669.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)")
![Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-ab238121--flow-cytometry-intracellular-img17561.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)")
Publications
Filter by
- eLife 9:2020Casein kinase 1G2 suppresses necroptosis-promoted testis aging by inhibiting receptor-interacting kinase 3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Expected | Expected | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Expected | Tested | Not recommended | Not recommended | Not recommended | Expected |
| Transfected cell lysate - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell lysate - Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell lysate - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation (PubMed:15077195, PubMed:15917222, PubMed:18794808, PubMed:19005213). Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate (By similarity). Involved in regulation of reactive oxygen species (ROS) levels (PubMed:37099597).
Alternative names
CK1G2, CSNK1G2, Casein kinase I isoform gamma-2, CKI-gamma 2
Recommended products
Rabbit Recombinant Monoclonal Casein Kinase 1 gamma 2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human, Transfected cell lysate - Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20918-13
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-ab238121--western-blot-img7791.jpg "Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)")
Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 20 µg
Lane 2: Rat testis tissue lysate at 20 µg
SecondaryLane 1: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Lane 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa
![Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-ab238121--flow-cytometry-intracellular-img80365.jpg "Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)")
Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized MEF (Mouse embryonic fibroblast (immortalized) cells labelling Casein Kinase 1 gamma 2 with ab238121 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-ab238121--immunoprecipitation-img5959.jpg "Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)")
Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
Casein Kinase 1 gamma 2 was immunoprecipitated from 0.35 mg mouse testis tissue lysate with ab238121 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238121 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse testis tissue lysate 10 ug
Lane 2: ab238121 IP in mouse testis tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab238121 in mouse testis tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds.
All lanes: Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
Predicted band size: 47 kDa
Observed band size: 50 kDa
![Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-ab238121--western-blot-img55669.jpg "Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)")
Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc- tag®, whole cell lysate at 20 µg
Lane 2: HEK-293T transfected with human Casein Kinase 1 gamma 2 (WT) expression vector containing a myc-tag®, whole cell lysate at 20 µg
Lane 3: HEK-293T transfected with mouse Casein Kinase 1 gamma 2 (WT) expression vector containing a myc-tag®, whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa
![Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-ab238121--flow-cytometry-intracellular-img17561.jpg "Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)")
Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells (transfected with myc-tagged Casein Kinase 1 gamma 2 construct) labelling Casein Kinase 1 gamma 2 with ab238121 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left).
A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
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