AB238121

Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Casein Kinase 1 gamma 2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human, Transfected cell lysate - Human samples. Cited in 1 publication.

View Alternative Names

CK1G2, CSNK1G2, Casein kinase I isoform gamma-2, CKI-gamma 2

5 Images

Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells (transfected with myc-tagged Casein Kinase 1 gamma 2 construct) labelling Casein Kinase 1 gamma 2 with ab238121 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized MEF (Mouse embryonic fibroblast (immortalized) cells labelling Casein Kinase 1 gamma 2 with ab238121 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Unknown

Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Casein Kinase 1 gamma 2 was immunoprecipitated from 0.35 mg mouse testis tissue lysate with ab238121 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238121 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse testis tissue lysate 10 ug

Lane 2 : ab238121 IP in mouse testis tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab238121 in mouse testis tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds.

All lanes:

Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)

Predicted band size: 47 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Rat testis tissue lysate at 20 µg

Secondary

Lane 1:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc- tag®, whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with human Casein Kinase 1 gamma 2 (WT) expression vector containing a myc-tag®, whole cell lysate at 20 µg

Lane 3:

HEK-293T transfected with mouse Casein Kinase 1 gamma 2 (WT) expression vector containing a myc-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 50 kDa

false

Carrier free

Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20918-13

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation (PubMed : 15077195, PubMed : 15917222, PubMed : 18794808, PubMed : 19005213). Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate (By similarity). Involved in regulation of reactive oxygen species (ROS) levels (PubMed : 37099597).

See full target information CSNK1G2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 9: PubMed33206046

2020

Casein kinase 1G2 suppresses necroptosis-promoted testis aging by inhibiting receptor-interacting kinase 3.

Applications

Species

Mouse

Dianrong Li,Youwei Ai,Jia Guo,Baijun Dong,Lin Li,Gaihong Cai,She Chen,Dan Xu,Fengchao Wang,Xiaodong Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13]

Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise