Rabbit Recombinant Monoclonal Casein Kinase 1 gamma 2 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.
Images
![Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (AB270040), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-bsa-and-azide-free-ab270040--flow-cytometry-intracellular-img80365.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (AB270040)")
![Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (AB270040), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-bsa-and-azide-free-ab270040--immunoprecipitation-img5959.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (AB270040)")
![Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (AB270040), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-bsa-and-azide-free-ab270040--flow-cytometry-intracellular-img17561.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (AB270040)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Expected | Expected | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Tested | Expected | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Predicted | Expected | Not recommended | Not recommended | Not recommended | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation (PubMed:15077195, PubMed:15917222, PubMed:18794808, PubMed:19005213). Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate (By similarity). Involved in regulation of reactive oxygen species (ROS) levels (PubMed:37099597).
Alternative names
CK1G2, CSNK1G2, Casein kinase I isoform gamma-2, CKI-gamma 2
Recommended products
Rabbit Recombinant Monoclonal Casein Kinase 1 gamma 2 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR20918-13
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab270040 is the carrier-free version of Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] ab238121.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-bsa-and-azide-free-ab270040--flow-cytometry-intracellular-img80365.jpg "Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)")
Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized MEF (Mouse embryonic fibroblast (immortalized) cells labelling Casein Kinase 1 gamma 2 with Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] ab238121 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] ab238121).
![Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-bsa-and-azide-free-ab270040--immunoprecipitation-img5959.jpg "Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)")
Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
Casein Kinase 1 gamma 2 was immunoprecipitated from 0.35 mg mouse testis tissue lysate with Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] ab238121 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] ab238121 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse testis tissue lysate 10 ug
Lane 2: Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] ab238121 IP in mouse testis tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] ab238121 in mouse testis tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] ab238121).
All lanes: Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] ab238121)
Predicted band size: 47 kDa
Observed band size: 50 kDa
![Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-1-gamma-2-antibody-epr20918-13-bsa-and-azide-free-ab270040--flow-cytometry-intracellular-img17561.jpg "Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)")
Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells (transfected with myc-tagged Casein Kinase 1 gamma 2 construct) labelling Casein Kinase 1 gamma 2 with Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] ab238121 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left).
A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] ab238121).
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