AB201990

Anti-Casein Kinase 2 beta antibody [2F12F3]

Name: Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990) | Abcam
Brand: Abcam Limited
SKU: AB201990
Price: 535 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Casein Kinase 2 beta antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat, Recombinant fragment - Human, Transfected cell lysate - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CSNK2B.

View Alternative Names

CK2N, G5A, CSNK2B, Casein kinase II subunit beta, CK II beta, Phosvitin, Protein G5a

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Casein Kinase 2 beta with ab201990 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Casein Kinase 2 beta with ab201990 at 1/200 dilution.

Flow Cytometry - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

Flow cytometric analysis of HeLa cells using ab201990 at 1/200 dilution (green) and negative control (red).

Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

Immunofluorescence analysis of MCF-7 cells using ab201990 at 1/200 dilution (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Supplier Data

Western blot - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

All lanes:

Western blot - Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990) at 1/500 dilution

All lanes:

Human Casein Kinase 2 beta (AA: FULL(1-215)) recombinant protein

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

All lanes:

Western blot - Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

Casein Kinase 2 beta (AA: FULL(1-215))-hIgGFc transfected HEK293 cell lysate

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

All lanes:

Western blot - Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990) at 1/500 dilution

Lane 1:

Hela cell lysate

Lane 2:

Jurkat cell lysate

Lane 3:

K562 cell lysate

Lane 4:

HepG2 cell lysate

Lane 5:

C6 cell lysate

Lane 6:

SK-N-SH cell lysate

Lane 7:

NTERA-2 cell lysate

Lane 8:

MCF-7 cell lysate

Lane 9:

NIH/3T3 cell lysate

Predicted band size: 24 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2F12F3

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt, WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CSNK2B.

P67870

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine (PubMed : 11239457, PubMed : 16818610). Participates in Wnt signaling (By similarity).. (Microbial infection) Upon infection with Epstein-Barr virus (EBV), the interaction with viral EBNA1 increases the association of CK2 with PML proteins, which increases PML phosphorylation by CK2, triggering the polyubiquitylation and degradation of PML (PubMed : 20719947, PubMed : 24216761). Seems to also suppress EBV reactivation by mediating ARK2N and JUN at the Z promoter which inhibits BZLF1 transcrition (PubMed : 31341047).

See full target information CSNK2B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of physiology 596:4299-4322 PubMed29917243

2018

Sex-specific activation of SK current by isoproterenol facilitates action potential triangulation and arrhythmogenesis in rabbit ventricles.

Applications

Unspecified application

Species

Unspecified reactive species

Mu Chen,Dechun Yin,Shuai Guo,Dong-Zhu Xu,Zhuo Wang,Zhenhui Chen,Michael Rubart-von der Lohe,Shien-Fong Lin,Thomas H Everett Iv,James N Weiss,Peng-Sheng Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Casein Kinase 2 beta antibody [2F12F3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

Flow Cytometry - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

Western blot - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

Western blot - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

Western blot - Anti-Casein Kinase 2 beta antibody [2F12F3] (AB201990)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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