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AB76025

Anti-Casein Kinase 2 beta antibody [EP1995Y]

5

(6 Reviews)

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(24 Publications)

Rabbit Recombinant Monoclonal Casein Kinase 2 beta antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Horse samples. Cited in 24 publications.

View Alternative Names

CK2N, G5A, CSNK2B, Casein kinase II subunit beta, CK II beta, Phosvitin, Protein G5a

5 Images
Flow Cytometry (Intracellular) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (AB76025)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (AB76025)

Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76025, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (AB76025)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (AB76025)

Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (AB76025)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (AB76025)

Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (AB76025)
  • WB

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Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (AB76025)

All lanes:

Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution

All lanes:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 24 kDa

Observed band size: 25 kDa

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Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (AB76025)
  • WB

CiteAb

Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (AB76025)

Casein Kinase 2 beta western blot using anti-Casein Kinase 2 beta antibody [EP1995Y] ab76025. Publication image and figure legend from Di Maira, G., Gentilini, A., et al., 2019, Oncogenesis, PubMed 31641101.

ab76025 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab76025 please see the product overview.

Expression and activity of CK2 in cholangiocarcinoma samples and CCA cells lines.a Expression of CK2 catalytic (CSNK2A1 and CSNK2A2) and regulatory (CSNK2B) subunits in human CCA tissues. CK2α, CK2α' and CK2β mRNA expression were significantly increased in tumor lesions (T) (n = 104) compared to surrounding matched normal liver tissue (SL) (n = 60) using transcriptome data of CCA patients35. Statistical significance was determined by nonparametric Mann–Whitney test. Individual patient data are shown, and the thick horizontal line indicates the mean of the values. §, p < 0.001 vs. T. b Relative quantitation of gene expression of CK2α, CK2α' and CK2β in cholangiocytes, HUCCT-1 and CCLP-1 cells. *p < 0.05 versus cholangiocytes. (c) Twenty μg (left panel) or 40 μg (right panel) of proteins from total cell lysate were analyzed by Western blot with the indicated antibodies. Tubulin was used as a loading control. d Activity of CK2 in cell lysates was measured using a CK2-specific peptide substrate. *p < 0.05 versus cholangiocytes

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  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Casein Kinase 2 beta antibody [EP1995Y]

  • Carrier free

    Anti-Casein Kinase 2 beta antibody [EP1995Y] - BSA and Azide free

  • 660 APC

    APC Anti-Casein Kinase 2 beta antibody [EP1995Y]

  • HRP

    HRP Anti-Casein Kinase 2 beta antibody [EP1995Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Casein Kinase 2 beta antibody [EP1995Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Casein Kinase 2 beta antibody [EP1995Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Casein Kinase 2 beta antibody [EP1995Y]

  • 578 PE

    PE Anti-Casein Kinase 2 beta antibody [EP1995Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Casein Kinase 2 beta antibody [EP1995Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Casein Kinase 2 beta antibody [EP1995Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1995Y

Isotype

IgG

Carrier free

No

Reacts with

Horse, Human

Applications

ICC/IF, IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "100-250 mg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Horse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine (PubMed : 11239457, PubMed : 16818610). Participates in Wnt signaling (By similarity).. (Microbial infection) Upon infection with Epstein-Barr virus (EBV), the interaction with viral EBNA1 increases the association of CK2 with PML proteins, which increases PML phosphorylation by CK2, triggering the polyubiquitylation and degradation of PML (PubMed : 20719947, PubMed : 24216761). Seems to also suppress EBV reactivation by mediating ARK2N and JUN at the Z promoter which inhibits BZLF1 transcrition (PubMed : 31341047).
See full target information CSNK2B
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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular biology 2:e125 PubMed38938901

2024

Cardiomyocyte intercellular signalling increases oxidative stress and reprograms the global- and phospho-proteome of cardiac fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Bethany Claridge,Alin Rai,Jarmon G Lees,Haoyun Fang,Shiang Y Lim,David W Greening

The Journal of clinical investigation 133: PubMed37022795

2023

Long noncoding RNA HIKER regulates erythropoiesis in Monge's disease via CSNK2B.

Applications

Unspecified application

Species

Unspecified reactive species

Priti Azad,Dan Zhou,Hung-Chi Tu,Francisco C Villafuerte,David Traver,Tariq M Rana,Gabriel G Haddad

Journal of immunology (Baltimore, Md. : 1950) 209:896-906 PubMed35914835

2022

Protein Kinase CK2 Controls CD8 T Cell Effector and Memory Function during Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yang,Hairong Wei,Gloria A Benavides,William J Turbitt,Jessica A Buckley,Xiaosen Ouyang,Lianna Zhou,Jianhua Zhang,Laurie E Harrington,Victor M Darley-Usmar,Hongwei Qin,Etty N Benveniste

ACS chemical biology 17:1111-1121 PubMed35467332

2022

Writing and Erasing O-GlcNAc on Casein Kinase 2 Alpha Alters the Phosphoproteome.

Applications

Unspecified application

Species

Unspecified reactive species

Paul A Schwein,Yun Ge,Bo Yang,Alexandria D'Souza,Alison Mody,Dacheng Shen,Christina M Woo

HGG advances 3:100111 PubMed35571680

2022

variants of cause a new intellectual disability-craniodigital syndrome by disrupting the canonical Wnt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Asif,Emrah Kaygusuz,Marwan Shinawi,Anna Nickelsen,Tzung-Chien Hsieh,Prerana Wagle,Birgit S Budde,Jennifer Hochscherf,Uzma Abdullah,Stefan Höning,Christian Nienberg,Dirk Lindenblatt,Angelika A Noegel,Janine Altmüller,Holger Thiele,Susanne Motameny,Nicole Fleischer,Idan Segal,Lynn Pais,Sigrid Tinschert,Nadra Nasser Samra,Juliann M Savatt,Natasha L Rudy,Chiara De Luca, Paola Fortugno,Susan M White,Peter Krawitz,Anna C E Hurst,Karsten Niefind,Joachim Jose,Francesco Brancati,Peter Nürnberg,Muhammad Sajid Hussain

Development (Cambridge, England) 149: PubMed35297994

2022

Establishment and characterization of equine mammary organoids using a method translatable to other non-traditional model species.

Applications

Unspecified application

Species

Unspecified reactive species

Arianna P Bartlett,Rebecca M Harman,Jennifer R Weiss,Gerlinde R Van de Walle

Nucleic acids research 50:3835-3851 PubMed35325203

2022

ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters.

Applications

Unspecified application

Species

Unspecified reactive species

Kihyun Park,Jian Zhong,Jin Sung Jang,Jihyun Kim,Hye-Jung Kim,Jeong-Heon Lee,Jaehoon Kim

Molecular cell 81:1515-1533.e5 PubMed33571423

2021

Poly-ADP-ribosylation drives loss of protein homeostasis in ATM and Mre11 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hoon Lee,Seung W Ryu,Nicolette A Ender,Tanya T Paull

Cells 10: PubMed33477590

2021

Contribution of the CK2 Catalytic Isoforms α and α' to the Glycolytic Phenotype of Tumor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Zonta,Christian Borgo,Camila Paz Quezada Meza,Ionica Masgras,Andrea Rasola,Mauro Salvi,Lorenzo A Pinna,Maria Ruzzene

International journal of molecular sciences 21: PubMed33114687

2020

White Adipose Tissue Expansion in Multiple Symmetric Lipomatosis Is Associated with Upregulation of CK2, AKT and ERK1/2.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Sanna,Christian Borgo,Chiara Compagnin,Francesca Favaretto,Vincenzo Vindigni,Mariangela Trento,Silvia Bettini,Alessandra Comin,Anna Belligoli,Massimo Rugge,Franco Bassetto,Arianna Donella-Deana,Roberto Vettor,Luca Busetto,Gabriella Milan
View all publications
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Product promise

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