Rabbit Recombinant Monoclonal Casein Kinase 2 beta antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Images
![Western blot - Anti-Casein Kinase 2 beta antibody [EPR1994] (AB133576), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-2-beta-antibody-epr1994-ab133576--western-blot-img116275.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Casein Kinase 2 beta antibody [EPR1994] (AB133576)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EPR1994] (AB133576), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-2-beta-antibody-epr1994-ab133576--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img3965.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EPR1994] (AB133576)")
![OI-RD Scanning - Anti-Casein Kinase 2 beta antibody [EPR1994] (AB133576), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-2-beta-antibody-epr1994-ab133576--oi-rd-scanning-img94801.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Casein Kinase 2 beta antibody [EPR1994] (AB133576)")
Publications
Filter by
- Nature communications 11:22892020A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung-Min Kim et. al.PubMed 32385263
- Journal of biological rhythms 34:144-1532019Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew D Beale et. al.PubMed 30898060
- Cellular physiology and biochemistry : internation 34:1701-102014Comparative proteomic profile of the human placenta in normal and fetal growth restriction subjects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhijing Miao et. al.PubMed 25401993
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Expected |
| Rat | Predicted | Not recommended | Not recommended | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Use a HRP/AP polymerized secondary antibody. Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine (PubMed:11239457, PubMed:16818610). Participates in Wnt signaling (By similarity). (Microbial infection) Upon infection with Epstein-Barr virus (EBV), the interaction with viral EBNA1 increases the association of CK2 with PML proteins, which increases PML phosphorylation by CK2, triggering the polyubiquitylation and degradation of PML (PubMed:20719947, PubMed:24216761). Seems to also suppress EBV reactivation by mediating ARK2N and JUN at the Z promoter which inhibits BZLF1 transcrition (PubMed:31341047).
Alternative names
CK2N, G5A, CSNK2B, Casein kinase II subunit beta, CK II beta, Phosvitin, Protein G5a
Recommended products
Rabbit Recombinant Monoclonal Casein Kinase 2 beta antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR1994
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.94 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-2-beta-antibody-epr1994-ab133576--western-blot-img116275.jpg "Western blot - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576)")
Western blot - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576)
All lanes: Western blot - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: SH-SY5Y cell lysate at 10 µg
Lane 4: HUVEC cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-2-beta-antibody-epr1994-ab133576--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img3965.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576)
Immunohistochemical analysis of Casein Kinase 2 beta in formalin fixed, paraffin embedded Human breast tissue, using ab133576 at a 1/50 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576), expandable thumbnail](https://content.abcam.com/products/images/casein-kinase-2-beta-antibody-epr1994-ab133576--oi-rd-scanning-img94801.jpg "OI-RD Scanning - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576)")
OI-RD Scanning - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com