Anti-CASK antibody [K56A/50] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] - BSA and Azide free (AB255764)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling CASK with ab252540 at 0.134μg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membranous staining on rat pancreas is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252540 for 30 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab252540).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] - BSA and Azide free (AB255764)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CASK with ab252540 at 0.134μg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membranous staining on mouse liver is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252540 for 30 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab252540).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] - BSA and Azide free (AB255764)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling CASK with ab252540 at 0.134μg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membranous and cytoplasmic staining on rat stomach is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252540 for 30 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab252540).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] - BSA and Azide free (AB255764)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling CASK with ab252540 at 0.134μg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membranous staining on rat liver is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252540 for 30 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab252540).

• WB

Unknown

Western blot - Anti-CASK antibody [K56A/50] - BSA and Azide free (AB255764)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lane 1 : 125 seconds; Lanes 2-5 : 26 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 15236336).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab252540).

All lanes:

Western blot - Anti-CASK antibody [K56A/50] (<a href='/en-us/products/primary-antibodies/cask-antibody-k56a-50-ab252540'>ab252540</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse cerebellum lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat cerebellum lysate at 20 µg

Secondary

Lane 1:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/100000 dilution

Lanes 2 - 5:

Peroxidase-Conjugated Goat anti-Mouse IgG（H+L） at 1/100000 dilution

Predicted band size: 105 kDa

Observed band size: 120 kDa

false

• WB

Lab

Western blot - Anti-CASK antibody [K56A/50] - BSA and Azide free (AB255764)

This data was developed using ab244393, the same antibody clone in a different buffer formulation.

Western blot : Anti-CASK antibody ab244393 staining at 0.4 µg/mL, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 105 kDa in Wild-type A549 cell lysates with no signal observed at this size in CASK knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CASK antibody (<a href='/en-us/products/primary-antibodies/cask-antibody-ab244393'>ab244393</a>) at 0.4 µg/mL

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

CASK knockout A549 at 20 µg

Lane 3:

Calu-3 at 20 µg

Lane 4:

HeLa at 20 µg

Lane 5:

THP-1 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 105 kDa

Observed band size: 105 kDa,36 kDa

false

• WB

Lab

Western blot - Anti-CASK antibody [K56A/50] - BSA and Azide free (AB255764)

This data was developed using ab252540, the same antibody clone in a different buffer formulation.

Western blot : Anti-CASK antibody [K56A/50] ab252540 staining at 1/1000 dilution, shown in green; Rabbit anti GAPDH ab181602 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 105 kDa in Wild-type A549 cell lysates with no signal observed at this size in CASK knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW and Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CASK antibody [K56A/50] (<a href='/en-us/products/primary-antibodies/cask-antibody-k56a-50-ab252540'>ab252540</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

CASK knockout A549 at 20 µg

Lane 3:

Calu-3 at 20 µg

Lane 4:

HeLa at 20 µg

Lane 5:

THP-1 at 20 µg

Secondary

All lanes:

Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 105 kDa

Observed band size: 105 kDa,37 kDa

false