Rabbit Recombinant Monoclonal Caspase-1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Images
![Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (AB322487), expandable thumbnail](https://content.abcam.com/products/images/caspase-1-antibody-epr25967-411-bsa-and-azide-free-detector-ab322487--sandwich-elisa-img438657.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (AB322487)")
![Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (AB322487), expandable thumbnail](https://content.abcam.com/products/images/caspase-1-antibody-epr25967-411-bsa-and-azide-free-detector-ab322487--sandwich-elisa-img438656.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (AB322487)")
![Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (AB322487), expandable thumbnail](https://content.abcam.com/products/images/caspase-1-antibody-epr25967-411-bsa-and-azide-free-detector-ab322487--sandwich-elisa-img438655.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (AB322487)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Mouse | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides (PubMed:21147462, PubMed:32109412). Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes (PubMed:21147462). Cleaves a tetrapeptide after an Asp residue at position P1 (PubMed:21147462). Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD (PubMed:32109412). In contrast to cleavage of interleukin IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32109412). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed:18667412, PubMed:22464733, PubMed:35705808). Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (By similarity).
Alternative names
Il1bc, Casp1, Caspase-1, CASP-1, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45, IL-1BC, ICE, IL-1 beta-converting enzyme
Rabbit Recombinant Monoclonal Caspase-1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25967-411
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab322487 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Caspase-1. The recommended pair for sandwich ELISA is:
Capture: Anti-Caspase-1 antibody [EPR25967-468] - BSA and Azide free (Capture) ab322486, Mouse Caspase-1 Capture Antibody (unconjugated)
Detector: ab322487, Mouse Caspase-1 Detector Antibody (unconjugated)
The reference range value is 15.63-2000 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (ab322487), expandable thumbnail](https://content.abcam.com/products/images/caspase-1-antibody-epr25967-411-bsa-and-azide-free-detector-ab322487--sandwich-elisa-img438657.jpg "Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (ab322487)")
Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (ab322487)
Interpolated concentration of native Caspase-1 was measured at different sample concentrations. Undiluted samples are as follows: stimulated J774A.1 cell extract (treated with 1 ug/mL LPS for 6 hrs) (31.25 µg/mL) and mock unstimulated J774A.1 cell extract (31.25 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
![Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (ab322487), expandable thumbnail](https://content.abcam.com/products/images/caspase-1-antibody-epr25967-411-bsa-and-azide-free-detector-ab322487--sandwich-elisa-img438656.jpg "Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (ab322487)")
Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (ab322487)
Interpolated concentration of native Caspase-1 was measured at different sample concentrations. Undiluted samples are as follows: stimulated J774A.1 supernatant (treated with 1 ug/mL LPS for 24 hrs) 12.5% and mock unstimulated J774A.1 supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
![Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (ab322487), expandable thumbnail](https://content.abcam.com/products/images/caspase-1-antibody-epr25967-411-bsa-and-azide-free-detector-ab322487--sandwich-elisa-img438655.jpg "Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (ab322487)")
Sandwich ELISA - Anti-Caspase-1 antibody [EPR25967-411] - BSA and Azide free (Detector) (ab322487)
Sandwich ELISA of Mouse Caspase-1 Antibody Pair - BSA and Azide free ab322485 with the capture antibody Anti-Caspase-1 antibody [EPR25967-468] - BSA and Azide free (Capture) ab322486 dilution at 2 ug/mL and detector antibody ab322487 dilution at 0.5 ug/mL.
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