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AB177475

Anti-Caspase-10/CASP-10 antibody [EPR10890]

4

(1 Review)

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(6 Publications)

Rabbit Recombinant Monoclonal Caspase-10/CASP-10 antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

MCH4, CASP10, Caspase-10, CASP-10, Apoptotic protease Mch-4, FAS-associated death domain protein interleukin-1B-converting enzyme 2, ICE-like apoptotic protease 4, FLICE2

5 Images
Flow Cytometry (Intracellular) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling Caspase 10/CASP-10 with ab177475 at 1/100 dilution (red) compared toa rabbit IgG negative control (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Caspase-10/CASP-10 with ab177475 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)
  • IP

Supplier Data

Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)

Western blot analysis on immunoprecipitation pellet from (1) K562 cell lysate or (2) 1XPBS (negative control) using ab177475 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

Predicted band size: 58 kDa

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Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)
  • WB

Supplier Data

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)

All lanes:

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Predicted band size: 58 kDa

false

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)
  • WB

Supplier Data

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)

Blocking and diluting buffer : 5% NFDM /TBST.

Exposure time :

Lane 1 & 2 : 7 seconds

Lane 3 & 4 : 3 seconds

Lanes 1 - 2:

Western blot - Anti-Pro Caspase-8 antibody [EPR162] (<a href='/en-us/products/primary-antibodies/pro-caspase-8-antibody-epr162-ab108333'>ab108333</a>) at 1/1000 dilution

Lanes 3 - 4:

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475) at 1/1000 dilution

Lanes 1 - 2:

His-tagged Human full-length Caspase 8 recombinant protein at 10 ng

Lanes 3 - 4:

His-tagged Human full-length Caspase 10 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60-70 kDa

false

  • Carrier free

    Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10890

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP8 and CASP9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.. Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis.. Isoform C is proteolytically inactive.
See full target information CASP10
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Archives of toxicology 98:1859-1875 PubMed38555327

2024

Effects of the nerve agent VX on hiPSC-derived motor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine Schaefers,Wolfgang Schmeißer,Harald John,Franz Worek,Theo Rein,Simone Rothmiller,Annette Schmidt

Nature communications 14:3742 PubMed37353488

2023

Systems-level analyses of protein-protein interaction network dysfunctions via epichaperomics identify cancer-specific mechanisms of stress adaptation.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Rodina,Chao Xu,Chander S Digwal,Suhasini Joshi,Yogita Patel,Anand R Santhaseela,Sadik Bay,Swathi Merugu,Aftab Alam,Pengrong Yan,Chenghua Yang,Tanaya Roychowdhury,Palak Panchal,Liza Shrestha,Yanlong Kang,Sahil Sharma,Justina Almodovar,Adriana Corben,Mary L Alpaugh,Shanu Modi,Monica L Guzman,Teng Fei,Tony Taldone,Stephen D Ginsberg,Hediye Erdjument-Bromage,Thomas A Neubert,Katia Manova-Todorova,Meng-Fu Bryan Tsou,Jason C Young,Tai Wang,Gabriela Chiosis

Cell biology international 45:154-163 PubMed33049089

2020

Long noncoding RNA PVT1 facilitates high glucose-induced cardiomyocyte death through the miR-23a-3p/CASP10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Feng-Rong Yu,Yin-Wen Xia,Shao-Bo Wang,Li-Hua Xiao

The Journal of pathology 248:476-487 PubMed30945298

2019

YWHAZ amplification/overexpression defines aggressive bladder cancer and contributes to chemo-/radio-resistance by suppressing caspase-mediated apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Cheng Yu,Chien-Feng Li,I-Hsuan Chen,Ming-Tsung Lai,Zi-Jun Lin,Praveen K Korla,Chee-Yin Chai,Grace Ko,Chih-Mei Chen,Tritium Hwang,Shan-Chih Lee,Jim J-C Sheu

PloS one 12:e0180230 PubMed28662157

2017

Sex-dependent aortic valve pathology in patients with rheumatic heart disease.

Applications

IHC-P

Species

Human

Feng Xiao,Rui Zheng,Di Yang,Kejiang Cao,Shijiang Zhang,Bingruo Wu,Yongfeng Shao,Bin Zhou

Oncology letters 12:1623-1629 PubMed27446483

2016

Role of caspase-10 in the death of acute leukemia cells.

Applications

WB

Species

Human

Wenjian Guo,Aishu Dong,Xiahui Pan,Xiaoji Lin,Ying Lin,Muqing He,Baoling Zhu,Liming Jin,Rongxing Yao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com