Rabbit Recombinant Monoclonal Caspase-10/CASP-10 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980), expandable thumbnail](https://content.abcam.com/products/images/caspase-10-casp-10-antibody-epr10890-bsa-and-azide-free-ab249980--immunoprecipitation-img131612.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980)")
![Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980), expandable thumbnail](https://content.abcam.com/products/images/caspase-10-casp-10-antibody-epr10890-bsa-and-azide-free-ab249980--western-blot-img56361.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980), expandable thumbnail](https://content.abcam.com/products/images/caspase-10-casp-10-antibody-epr10890-bsa-and-azide-free-ab249980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55543.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980)")
![Flow Cytometry (Intracellular) - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980), expandable thumbnail](https://content.abcam.com/products/images/caspase-10-casp-10-antibody-epr10890-bsa-and-azide-free-ab249980--flow-cytometry-intracellular-img13360.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980)")
![Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980), expandable thumbnail](https://content.abcam.com/products/images/caspase-10-casp-10-antibody-epr10890-bsa-and-azide-free-ab249980--western-blot-img438509.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP8 and CASP9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis. Isoform C is proteolytically inactive.
Alternative names
MCH4, CASP10, Caspase-10, CASP-10, Apoptotic protease Mch-4, FAS-associated death domain protein interleukin-1B-converting enzyme 2, ICE-like apoptotic protease 4, FLICE2
Recommended products
Rabbit Recombinant Monoclonal Caspase-10/CASP-10 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10890
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249980 is the carrier-free version of Anti-Caspase-10/CASP-10 antibody [EPR10890] ab177475.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980), expandable thumbnail](https://content.abcam.com/products/images/caspase-10-casp-10-antibody-epr10890-bsa-and-azide-free-ab249980--immunoprecipitation-img131612.jpg "Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980)")
Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980)
This data was developed using Anti-Caspase-10/CASP-10 antibody [EPR10890] ab177475, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) K562 cell lysate or (2) 1XPBS (negative control) using Anti-Caspase-10/CASP-10 antibody [EPR10890] ab177475 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (Anti-Caspase-10/CASP-10 antibody [EPR10890] ab177475)
Predicted band size: 58 kDa
![Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980), expandable thumbnail](https://content.abcam.com/products/images/caspase-10-casp-10-antibody-epr10890-bsa-and-azide-free-ab249980--western-blot-img56361.jpg "Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980)")
Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980)
This data was developed using Anti-Caspase-10/CASP-10 antibody [EPR10890] ab177475, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (Anti-Caspase-10/CASP-10 antibody [EPR10890] ab177475) at 1/1000 dilution
Lane 1: K562 cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Predicted band size: 58 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980), expandable thumbnail](https://content.abcam.com/products/images/caspase-10-casp-10-antibody-epr10890-bsa-and-azide-free-ab249980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55543.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980)
This data was developed using Anti-Caspase-10/CASP-10 antibody [EPR10890] ab177475, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Caspase-10/CASP-10 with Anti-Caspase-10/CASP-10 antibody [EPR10890] ab177475 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980), expandable thumbnail](https://content.abcam.com/products/images/caspase-10-casp-10-antibody-epr10890-bsa-and-azide-free-ab249980--flow-cytometry-intracellular-img13360.jpg "Flow Cytometry (Intracellular) - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980)")
Flow Cytometry (Intracellular) - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980)
This data was developed using Anti-Caspase-10/CASP-10 antibody [EPR10890] ab177475, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized K562 cells labeling Caspase 10/CASP-10 with Anti-Caspase-10/CASP-10 antibody [EPR10890] ab177475 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
![Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980), expandable thumbnail](https://content.abcam.com/products/images/caspase-10-casp-10-antibody-epr10890-bsa-and-azide-free-ab249980--western-blot-img438509.jpg "Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980)")
Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (ab249980)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Caspase-10/CASP-10 antibody [EPR10890] ab177475).
Blocking and diluting buffer: 5% NFDM /TBST.
Exposure time:
Lane 1 & 2: 7 seconds
Lane 3 & 4: 3 seconds
Lanes 1 - 2: Western blot - Anti-Pro Caspase-8 antibody [EPR162] (Anti-Pro Caspase-8 antibody [EPR162] ab108333) at 1/1000 dilution
Lanes 3 - 4: Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (Anti-Caspase-10/CASP-10 antibody [EPR10890] ab177475) at 1/1000 dilution
Lanes 1 - 2: His-tagged Human full-length Caspase 8 recombinant protein at 10 ng
Lanes 3 - 4: His-tagged Human full-length Caspase 10 recombinant protein at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 60-70 kDa
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