Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980)

This data was developed using ab177475, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized K562 cells labeling Caspase 10/CASP-10 with ab177475 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980)

This data was developed using ab177475, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Caspase-10/CASP-10 with ab177475 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980)

This data was developed using ab177475, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (1) K562 cell lysate or (2) 1XPBS (negative control) using ab177475 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (<a href='/en-us/products/primary-antibodies/caspase-10-casp-10-antibody-epr10890-ab177475'>ab177475</a>)

Predicted band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980)

This data was developed using ab177475, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (<a href='/en-us/products/primary-antibodies/caspase-10-casp-10-antibody-epr10890-ab177475'>ab177475</a>) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Predicted band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free (AB249980)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177475).

Blocking and diluting buffer : 5% NFDM /TBST.

Exposure time :

Lane 1 & 2 : 7 seconds

Lane 3 & 4 : 3 seconds

Lanes 1 - 2:

Western blot - Anti-Pro Caspase-8 antibody [EPR162] (<a href='/en-us/products/primary-antibodies/pro-caspase-8-antibody-epr162-ab108333'>ab108333</a>) at 1/1000 dilution

Lanes 3 - 4:

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (<a href='/en-us/products/primary-antibodies/caspase-10-casp-10-antibody-epr10890-ab177475'>ab177475</a>) at 1/1000 dilution

Lanes 1 - 2:

His-tagged Human full-length Caspase 8 recombinant protein at 10 ng

Lanes 3 - 4:

His-tagged Human full-length Caspase 10 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60-70 kDa

false