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AB235180

Anti-Caspase-12 antibody

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(2 Publications)

Rabbit Polyclonal Caspase-12 antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

Caspase-12, CASP-12, Casp12

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-12 antibody (AB235180)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-12 antibody (AB235180)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling Caspase-12 with ab235180 at 15 μg/ml , followed by Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 μg/ml for 1 hour at RT (green).

Expected localization : Endoplasmic reticulum. The nuclear counter stain is DAPI (blue).

Panel A : DAPI.

Panel B : ab235180 + secondary antibody.

Panel C : Merge of panel A and B.

Panel D : Secondary only.

Western blot - Anti-Caspase-12 antibody (AB235180)
  • WB

Supplier Data

Western blot - Anti-Caspase-12 antibody (AB235180)

All lanes:

Western blot - Anti-Caspase-12 antibody (ab235180) at 0.5 µg/mL

All lanes:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 35 µg

Secondary

All lanes:

Goat anti-rabbit IgG [H&L] HRP at 1/40000 dilution

Predicted band size: 48 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "15 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the activation cascade of caspases responsible for apoptosis execution.
See full target information Casp12
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed38067151

2023

Antifibrotic Effect of Selenium-Containing Nanoparticles on a Model of TAA-Induced Liver Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Elena G Varlamova,Michail Victorovich Goltyaev,Vladimir Vladimirovich Rogachev,Sergey V Gudkov,Elena V Karaduleva,Egor A Turovsky

International journal of biological sciences 19:1228-1240 PubMed36923943

2023

Transmembrane BAX inhibitor motif containing 6 suppresses presenilin-2 to preserve mitochondrial integrity after myocardial ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ma,Lihan Liao,Na Zhou,Huikang Tao,Hao Zhou,Ying Tan,Weidan Chen,Fan Cao,Xinxin Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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