Rabbit Polyclonal Caspase-12 antibody. Suitable for WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 124 publications. Immunogen corresponding to Synthetic Peptide within Mouse Casp12.
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- Experimental and therapeutic medicine 26:5752023IGF2BP2‑dependent STIM1 inhibition protects against LPS‑induced pneumonia by alleviating endoplasmic reticulum stress and the inflammatory response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhou,Qigang Dai,Ning Su,Zhihui Liu,Jinxing HuPubMed 38023363
- Signal transduction and targeted therapy 8:3662023Non-small cell lung cancers (NSCLCs) oncolysis using coxsackievirus B5 and synergistic DNA-damage response inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBopei Cui,Lifang Song,Qian Wang,Kelei Li,Qian He,Xing Wu,Fan Gao,Mingchen Liu,Chaoqiang An,Qiushuang Gao,Chaoying Hu,Xiaotian Hao,Fangyu Dong,Jiuyue Zhou,Dong Liu,Ziyang Song,Xujia Yan,Jialu Zhang,Yu Bai,Qunying Mao,Xiaoming Yang,Zhenglun LiangPubMed 37743418
- Experimental and therapeutic medicine 26:5082023Zinc oxide nanoparticles inhibit malignant progression and chemotherapy resistance of ovarian cancer cells by activating endoplasmic reticulum stress and promoting autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenli Gu,Caihong YangPubMed 37840563
- Diabetes & vascular disease research 20:147916412311971062023Dapagliflozin attenuates cardiac remodeling and dysfunction in rats with β-adrenergic receptor overactivation through restoring calcium handling and suppressing cardiomyocyte apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Liu,Jinchun Wu,Shaobo Shi,Bo Cui,Feng Xiong,Shuang Yang,Min YanPubMed 37589258
- Pharmacological reports : PR 75:293-3192023Modulation of the endoplasmic reticulum stress and unfolded protein response mitigates the behavioral effects of early-life stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Solarz-Andrzejewska,Iwona Majcher-Maślanka,Joanna Kryst,Agnieszka ChocykPubMed 36843201
- International journal of molecular sciences 23:2022Activating PPARβ/δ Protects against Endoplasmic Reticulum Stress-Induced Astrocytic Apoptosis via UCP2-Dependent Mitophagy in Depressive Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Ji,Shangze Li,Zikai Jiang,Jianbing Yu,Yuqin Sun,Zhenyu Cai,Yinfeng Dong,Xiulan SunPubMed 36142731
- Frontiers in microbiology 13:9009192022EspF of Enterohemorrhagic Enhances Apoptosis Endoplasmic Reticulum Stress in Intestinal Epithelial Cells: An Isobaric Tags for Relative and Absolute Quantitation-Based Comparative Proteomic Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangyu Wang,Kaina Yan,Muqing Fu,Song Liang,Haiyi Zhao,Changzhu Fu,Lan Yang,Zhihong Song,Dayong Sun,Chengsong WanPubMed 35847082
- Molecular medicine reports 26:2022Hyperoside ameliorates TNF‑α‑induced inflammation, ECM degradation and ER stress‑mediated apoptosis via the SIRT1/NF‑κB and Nrf2/ARE signaling pathways .Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Xie,Jun Yuan,Ling Mei,Ping Li,Ruijie PanPubMed 35730622
- Bioengineered 13:13739-137512022Glucose fluctuation promotes cardiomyocyte apoptosis by triggering endoplasmic reticulum (ER) stress signaling pathway and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Da Wu,Ying Liu,Feng Li,Jia-Yi Chen,Jie Zhang,Ling-Ling Qian,Ru-Xing WangPubMed 35707846
- Cell communication and signaling : CCS 20:762022Podocyte specific deletion of PKM2 ameliorates LPS-induced podocyte injury through beta-catenin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammed Alquraishi,Samah Chahed,Dina Alani,Dexter L Puckett,Presley D Dowker,Katelin Hubbard,Yi Zhao,Ji Yeon Kim,Laurentia Nodit,Huma Fatima,Dallas Donohoe,Brynn Voy,Winyoo Chowanadisai,Ahmed BettaiebPubMed 35637461
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Casp12. The exact immunogen used to generate this antibody is proprietary information. Database link O08736
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes This antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 kDa) in the appropriate lysates. |
Species Rat | Dilution info 0.5-2 µg/mL | Notes This antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 kDa) in the appropriate lysates. |
Species Mouse | Dilution info 0.5-2 µg/mL | Notes This antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 kDa) in the appropriate lysates. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.5-2 µg/mL | Notes - |
Species Human | Dilution info 0.5-2 µg/mL | Notes - |
Species Mouse | Dilution info 0.5-2 µg/mL | Notes - |
Associated Products
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Target data
Function
May function as a negative regulator of inflammatory responses and innate immunity. May reduce cytokine release in response to bacterial lipopolysaccharide during infection. Reduces activation of NF-kappa-B in response to TNF (PubMed:15129283). May lack protease activity (Probable).
Alternative names
UNQ9415/PRO34398, CASP12, Inactive caspase-12, CASP-12
Recommended products
Rabbit Polyclonal Caspase-12 antibody. Suitable for WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 124 publications. Immunogen corresponding to Synthetic Peptide within Mouse Casp12.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Casp12. The exact immunogen used to generate this antibody is proprietary information. Database link O08736
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody reacts with both the propeptide and cleaved forms of Caspase-12.
- Concentration
- Purification notes
Caspase-12 Antibody is (Large)affinity chromatography purified via peptide column.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
This image is courtesy of a customer review submitted by Karine Thibault, CNRS, Paris, FranceWestern blot - Anti-Caspase-12 antibody (ab62484)
The full length Casp12 (42 kDa) observed in control rat was cleaved into an active form with a size of 38 kDa in treated rat
All lanes: Western blot - Anti-Caspase-12 antibody (ab62484) at 1/2000 dilution
Lane 1: Rat dorsal root ganglion at 15 µg
Lane 2: Treated rat dorsal root ganglion at 15 µg
SecondaryAll lanes: anti-rabbit HRP-linked secondary antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 40-45 kDa
Exposure time: 2min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (ab62484)
Caspase-12 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat brain tissue using rabbit Anti-Caspase-12 antibody
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Caspase-12 antibody (ab62484) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-Caspase-12 antibody (ab62484)
Caspase-12 Western blot staining using rabbit Anti-Caspase-12 antibody
1h incubation at RT in 5% NFDM/TBST
All lanes: Western blot - Anti-Caspase-12 antibody (ab62484) at 1 µg/mL
Lane 1: Rat liver tissue lysate at 15 µg
Lane 2: Rat skin tissue lysate at 15 µg
Lane 3: Rat spleen tissue lysate at 15 µg
Lane 4: Rat heart tissue lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 46 kDa
Western blot - Anti-Caspase-12 antibody (ab62484)
Caspase-12 Western blot staining using rabbit Anti-Caspase-12 antibody
1h incubation at RT in 5% NFDM/TBST
All lanes: Western blot - Anti-Caspase-12 antibody (ab62484) at 0.5 µg/mL
Lane 1: Human heart tissue lysate at 15 µg
Lane 2: Mouse heart tissue lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (ab62484)
Caspase-12 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse brain tissue using rabbit Anti-Caspase-12 antibody
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Caspase-12 antibody (ab62484) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (ab62484)
Caspase-12 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human colon tissue using rabbit Anti-Caspase-12 antibody
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Caspase-12 antibody (ab62484) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-Caspase-12 antibody (ab62484)
Caspase-12 Western blot staining using rabbit Anti-Caspase-12 antibody
1h incubation at RT in 5% NFDM/TBST
All lanes: Western blot - Anti-Caspase-12 antibody (ab62484) at 1 µg/mL
Lane 1: Human spleen tissue lysate at 15 µg
Lane 2: Human heart tissue lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 46 kDa
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