Anti-Caspase-12 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (AB62484)

Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Caspase-12 antibody (ab62484) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (AB62484)

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Caspase-12 antibody (ab62484) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (AB62484)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Caspase-12 antibody (ab62484) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• WB

Supplier Data

Western blot - Anti-Caspase-12 antibody (AB62484)

1h incubation at RT in 5% NFDM/TBST

All lanes:

Western blot - Anti-Caspase-12 antibody (ab62484) at 0.5 µg/mL

Lane 1:

Human heart tissue lysate at 15 µg

Lane 2:

Mouse heart tissue lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 46 kDa

false

• WB

Supplier Data

Western blot - Anti-Caspase-12 antibody (AB62484)

1h incubation at RT in 5% NFDM/TBST

All lanes:

Western blot - Anti-Caspase-12 antibody (ab62484) at 1 µg/mL

Lane 1:

Human spleen tissue lysate at 15 µg

Lane 2:

Human heart tissue lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 46 kDa

false

• WB

AbReview21056****

Western blot - Anti-Caspase-12 antibody (AB62484)

The full length Casp12 (42 kDa) observed in control rat was cleaved into an active form with a size of 38 kDa in treated rat

All lanes:

Western blot - Anti-Caspase-12 antibody (ab62484) at 1/2000 dilution

Lane 1:

Rat dorsal root ganglion at 15 µg

Lane 2:

Treated rat dorsal root ganglion at 15 µg

Secondary

All lanes:

anti-rabbit HRP-linked secondary antibody at 1/10000 dilution

Predicted band size: 38 kDa

Observed band size: 40-45 kDa

true

Exposure time: 2min

This image is courtesy of an abreview submitted by Karine Thibault, CNRS, Paris, France

• WB

Supplier Data

Western blot - Anti-Caspase-12 antibody (AB62484)

1h incubation at RT in 5% NFDM/TBST

All lanes:

Western blot - Anti-Caspase-12 antibody (ab62484) at 1 µg/mL

Lane 1:

Rat liver tissue lysate at 15 µg

Lane 2:

Rat skin tissue lysate at 15 µg

Lane 3:

Rat spleen tissue lysate at 15 µg

Lane 4:

Rat heart tissue lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 46 kDa

false