Anti-Caspase-12 antibody [EPR27005-83] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Caspase-12 antibody [EPR27005-83] - BSA and Azide free (AB315272)

This data was developed using ab315271, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the lower MW band at approximately 28 kDa is unknown.

It was reported that the full-length form only exists in a sub-set of people with African descent and is rare elsewhere (PMID : 16532395, PMID : 15129283, PMID : 16917906).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Caspase-12 antibody [EPR27005-83] (<a href='/en-us/products/primary-antibodies/caspase-12-antibody-epr27005-83-ab315271'>ab315271</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting Caspase-12 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 39 kDa,35 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Caspase-12 antibody [EPR27005-83] - BSA and Azide free (AB315272)

This data was developed using ab315271, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands between 20 kDa and 28 kDa are unknown.

All lanes:

Western blot - Anti-Caspase-12 antibody [EPR27005-83] (<a href='/en-us/products/primary-antibodies/caspase-12-antibody-epr27005-83-ab315271'>ab315271</a>) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 53 kDa,45 kDa

false

Exposure time: 125s

• WB

Supplier Data

Western blot - Anti-Caspase-12 antibody [EPR27005-83] - BSA and Azide free (AB315272)

This data was developed using ab315271, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 12097332, PMID : 12232799).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Caspase-12 antibody [EPR27005-83] (<a href='/en-us/products/primary-antibodies/caspase-12-antibody-epr27005-83-ab315271'>ab315271</a>) at 1/1000 dilution

Lane 1:

Untreated MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Lane 2:

MEF treated with 300 nM Thapsigargin for 18 hours whole cell lysate at 20 µg

Lane 3:

Rat heart tissue lysate at 20 µg

Lane 4:

Rat lung tissue lysate at 20 µg

Lane 5:

L-929 (mouse connective tissue fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 53 kDa,45 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Caspase-12 antibody [EPR27005-83] - BSA and Azide free (AB315272)

This data was developed using ab315271, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Caspase-12 antibody [EPR27005-83] (<a href='/en-us/products/primary-antibodies/caspase-12-antibody-epr27005-83-ab315271'>ab315271</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 7 µg

Lane 2:

293T cells transfected with a human CASP12 expression vector containing a myc-His-tag®, whole cell lysate at 7 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 39 kDa,15 kDa

false

Exposure time: 1s