Rabbit Recombinant Monoclonal Caspase-14 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.
Images
![Western blot - Anti-Caspase-14 antibody [EPR12927] (AB174847), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-ab174847--western-blot-img6726.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-14 antibody [EPR12927] (AB174847)")
![Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] (AB174847), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-ab174847--immunocytochemistry-immunofluorescence-img69245.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] (AB174847)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (AB174847), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-ab174847--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17318.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (AB174847)")
![Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (AB174847), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-ab174847--immunoprecipitation-img130133.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (AB174847)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (AB174847), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-ab174847--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101268.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (AB174847)")
Publications
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- International journal of molecular sciences 24:2023Effect of Hypertrophic Scar Fibroblast-Derived Exosomes on Keratinocytes of Normal Human Skin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Song Cui,So Young Joo,Seung Yeol Lee,Yoon Soo Cho,Dong Hyun Kim,Cheong Hoon SeoPubMed 37047109
- Bioengineering (Basel, Switzerland) 9:2022Keratin Promotes Differentiation of Keratinocytes Seeded on Collagen/Keratin Hydrogels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKameel Zuniga,Neda Ghousifam,John Sansalone,Kris Senecal,Mark Van Dyke,Marissa Nichole RylanderPubMed 36290526
- Scientific reports 11:172962021Effect of extracorporeal shock wave therapy on keratinocytes derived from human hypertrophic scars.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Song Cui,So Young Joo,Yoon Soo Cho,Ji Heon Park,Yu Mi Ro,June-Bum Kim,Cheong Hoon SeoPubMed 34453089
- Proteomics. Clinical applications 13:e19000292019Moonlighting Proteins and Cardiopathy in the Spatial Response of MCF-7 Breast Cancer Cells to Tamoxifen.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbdulrab Ahmed M Alkhanjaf et. al.PubMed 31282103
- Cancer science 109:373-3832017PRDM14 directly interacts with heat shock proteins HSP90α and glucose-regulated protein 78.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiharu Moriya,Hiroaki Taniguchi,Satoru Nagatoishi,Hisayoshi Igarashi,Kouhei Tsumoto,Kohzoh ImaiPubMed 29178343
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed:15556625). Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity). In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed:16854378, PubMed:19960512). Involved in processing of prosaposin in the epidermis (By similarity). May be involved in retinal pigment epithelium cell barrier function (PubMed:25121097). Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed:24743736).
Alternative names
Caspase-14, CASP-14, CASP14
Recommended products
Rabbit Recombinant Monoclonal Caspase-14 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12927
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Caspase-14 antibody [EPR12927] (ab174847), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-ab174847--western-blot-img6726.jpg "Western blot - Anti-Caspase-14 antibody [EPR12927] (ab174847)")
Western blot - Anti-Caspase-14 antibody [EPR12927] (ab174847)
All lanes: Western blot - Anti-Caspase-14 antibody [EPR12927] (ab174847) at 1/1000 dilution
Lane 1: MCF7 cell lysate at 10 µg
Lane 2: ECV-304 cell lysate at 10 µg
Lane 3: HACAT cell lysate at 10 µg
Lane 4: Human skin lysate at 10 µg
Predicted band size: 28 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] (ab174847), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-ab174847--immunocytochemistry-immunofluorescence-img69245.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] (ab174847)")
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] (ab174847)
Immunofluorescent staining of HaCaT cells labeling Caspase-14 (green) with ab174847 at 1:250 dilution, and labeling tubulin (red) with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1:500 (2 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used at 1:200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) was used at 1:200.
-ve control 1– Rabbit primary and anti-mouse secondary antibody. -ve control 2 – Mouse primary antibody and anti-rabbit secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (ab174847), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-ab174847--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17318.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (ab174847)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (ab174847)
Immunohistochemical analysis of paraffin-embedded human fetal skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (ab174847), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-ab174847--immunoprecipitation-img130133.jpg "Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (ab174847)")
Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (ab174847)
Western blot analysis on immunoprecipitation pellet from human fetal skin lysate (lane 1) or 1X PBS (lane 2, negative control) using ab174847 at 1:10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (ab174847)
Predicted band size: 28 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (ab174847), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-ab174847--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101268.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (ab174847)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (ab174847)
Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] (ab174847), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-ab174847--flow-cytometry-intracellular-img39807.jpg "Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] (ab174847)")
Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] (ab174847)
Intracellular flow cytometric analysis of permeabilized HACAT cells labeling Caspase-14using ab174847 at a 1/10 dilution (red), or rabbit IgG negative control (green).
![Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] (ab174847), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-ab174847--flow-cytometry-intracellular-img39807.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] (ab174847)")
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