Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835)

This data was developed using ab174847, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HACAT cells labeling Caspase-14 using ab174847 at a 1/10 dilution (red), or rabbit IgG negative control (green).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835)

This data was developed using ab174847, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of HaCaT cells labeling Caspase-14 (green) with ab174847 at 1 : 250 dilution, and labeling tubulin (red) with ab7291 at 1 : 500 (2 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used at 1 : 200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) was used at 1 : 200.

-ve control 1– Rabbit primary and anti-mouse secondary antibody. -ve control 2 – Mouse primary antibody and anti-rabbit secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835)

This data was developed using ab174847, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human fetal skin tissue labeling Caspase-14 using ab174847 at a 1 : 100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835)

This data was developed using ab174847, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Caspase-14 using ab174847 at a 1 : 100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835)

This data was developed using ab174847, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from human fetal skin lysate (lane 1) or 1X PBS (lane 2, negative control) using ab174847 at 1 : 10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (<a href='/en-us/products/primary-antibodies/caspase-14-antibody-epr12927-ab174847'>ab174847</a>)

Predicted band size: 28 kDa

false

• WB

Supplier Data

Western blot - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835)

This data was developed using ab174847, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Caspase-14 antibody [EPR12927] (<a href='/en-us/products/primary-antibodies/caspase-14-antibody-epr12927-ab174847'>ab174847</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

ECV-304 cell lysate at 10 µg

Lane 3:

HACAT cell lysate at 10 µg

Lane 4:

Human skin lysate at 10 µg

Predicted band size: 28 kDa

false