Rabbit Recombinant Monoclonal Caspase-14 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-bsa-and-azide-free-ab249835--western-blot-img6726.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835)")
![Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-bsa-and-azide-free-ab249835--immunocytochemistry-immunofluorescence-img69245.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-bsa-and-azide-free-ab249835--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17318.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835)")
![Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-bsa-and-azide-free-ab249835--immunoprecipitation-img130133.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-bsa-and-azide-free-ab249835--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101268.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (AB249835)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed:15556625). Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity). In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed:16854378, PubMed:19960512). Involved in processing of prosaposin in the epidermis (By similarity). May be involved in retinal pigment epithelium cell barrier function (PubMed:25121097). Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed:24743736).
Alternative names
Caspase-14, CASP-14, CASP14
Recommended products
Rabbit Recombinant Monoclonal Caspase-14 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12927
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249835 is the carrier-free version of Anti-Caspase-14 antibody [EPR12927] ab174847.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-bsa-and-azide-free-ab249835--western-blot-img6726.jpg "Western blot - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)")
Western blot - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)
This data was developed using Anti-Caspase-14 antibody [EPR12927] ab174847, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Caspase-14 antibody [EPR12927] (Anti-Caspase-14 antibody [EPR12927] ab174847) at 1/1000 dilution
Lane 1: MCF7 cell lysate at 10 µg
Lane 2: ECV-304 cell lysate at 10 µg
Lane 3: HACAT cell lysate at 10 µg
Lane 4: Human skin lysate at 10 µg
Predicted band size: 28 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-bsa-and-azide-free-ab249835--immunocytochemistry-immunofluorescence-img69245.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)")
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)
This data was developed using Anti-Caspase-14 antibody [EPR12927] ab174847, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HaCaT cells labeling Caspase-14 (green) with Anti-Caspase-14 antibody [EPR12927] ab174847 at 1:250 dilution, and labeling tubulin (red) with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1:500 (2 µg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used at 1:200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) was used at 1:200.-ve control 1– Rabbit primary and anti-mouse secondary antibody. -ve control 2 – Mouse primary antibody and anti-rabbit secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-bsa-and-azide-free-ab249835--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17318.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)
This data was developed using Anti-Caspase-14 antibody [EPR12927] ab174847, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human fetal skin tissue labeling Caspase-14 using Anti-Caspase-14 antibody [EPR12927] ab174847 at a 1:100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-bsa-and-azide-free-ab249835--immunoprecipitation-img130133.jpg "Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)")
Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)
This data was developed using Anti-Caspase-14 antibody [EPR12927] ab174847, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from human fetal skin lysate (lane 1) or 1X PBS (lane 2, negative control) using Anti-Caspase-14 antibody [EPR12927] ab174847 at 1:10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (Anti-Caspase-14 antibody [EPR12927] ab174847)
Predicted band size: 28 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-bsa-and-azide-free-ab249835--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101268.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)
This data was developed using Anti-Caspase-14 antibody [EPR12927] ab174847, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Caspase-14 using Anti-Caspase-14 antibody [EPR12927] ab174847 at a 1:100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-bsa-and-azide-free-ab249835--flow-cytometry-intracellular-img39807.jpg "Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)")
Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)
This data was developed using Anti-Caspase-14 antibody [EPR12927] ab174847, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized HACAT cells labeling Caspase-14 using Anti-Caspase-14 antibody [EPR12927] ab174847 at a 1/10 dilution (red), or rabbit IgG negative control (green).
![Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835), expandable thumbnail](https://content.abcam.com/products/images/caspase-14-antibody-epr12927-bsa-and-azide-free-ab249835--flow-cytometry-intracellular-img39807.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)")
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