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AB179520

Anti-Caspase-2 antibody [EPR16796]

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(10 Publications)

Rabbit Recombinant Monoclonal Caspase-2 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 10 publications.

View Alternative Names

ICH1, NEDD2, CASP2, Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, Protease ICH-1, NEDD-2

4 Images
Western blot - Anti-Caspase-2 antibody [EPR16796] (AB179520)
  • WB

Supplier Data

Western blot - Anti-Caspase-2 antibody [EPR16796] (AB179520)

The 33KDa band (cleaved caspase 2) contains subunit p18.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilution

All lanes:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 51 kDa

false

Exposure time: 3min

Western blot - Anti-Caspase-2 antibody [EPR16796] (AB179520)
  • WB

Lab

Western blot - Anti-Caspase-2 antibody [EPR16796] (AB179520)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Caspase-2 knockout HAP1 cell lysate (20 μg)

Lanes 1 and 2 : Merged signal (red and green). Green - ab17952 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.

ab179520 was shown to recognize Caspase-2 when Caspase-2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase-2 knockout samples were subjected to SDS-PAGE. ab179520 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)

Predicted band size: 51 kDa

false

Western blot - Anti-Caspase-2 antibody [EPR16796] (AB179520)
  • WB

Supplier Data

Western blot - Anti-Caspase-2 antibody [EPR16796] (AB179520)

The 33KDa band (cleaved caspase 2) contains subunit p18.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 4uM camptothecin for 4 hours at 10 µg

Lane 2:

Untreated HeLa whole cell lysate at 10 µg

Lane 3:

C6 (Rat glial tumor cells) whole cell lysate treated with 1uM staurosporine for 4 hours at 10 µg

Lane 4:

Untreated C6 whole cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 33 kDa,48 kDa

false

Exposure time: 3min

Western blot - Anti-Caspase-2 antibody [EPR16796] (AB179520)
  • WB

Supplier Data

Western blot - Anti-Caspase-2 antibody [EPR16796] (AB179520)

The 33KDa band (cleaved caspase 2) contains subunit p18.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/1000 dilution

All lanes:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 33 kDa,48 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16796

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Is a regulator of the cascade of caspases responsible for apoptosis execution (PubMed : 11156409, PubMed : 15073321, PubMed : 8087842). Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival (PubMed : 15073321). Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (PubMed : 15073321).. Isoform 1. Acts as a positive regulator of apoptosis.. Isoform 2. Acts as a negative regulator of apoptosis.. Isoform 3. May function as an endogenous apoptosis inhibitor that antagonizes caspase activation and cell death.
See full target information CASP2
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Neurology international 15:980-993 PubMed37606396

2023

FTD/ALS Type 7-Associated Thr104Asn Mutation of CHMP2B Blunts Neuronal Process Elongation, and Is Recovered by Knockdown of Arf4, the Golgi Stress Regulator.

Applications

Unspecified application

Species

Unspecified reactive species

Remina Shirai,Mizuka Cho,Mikinori Isogai,Shoya Fukatsu,Miyu Okabe,Maho Okawa,Yuki Miyamoto,Tomohiro Torii,Junji Yamauchi

eLife 11: PubMed36189922

2022

S6K1 phosphorylates Cdk1 and MSH6 to regulate DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Adi Amar-Schwartz,Vered Ben Hur,Amina Jbara,Yuval Cohen,Georgina D Barnabas,Eliran Arbib,Zahava Siegfried,Bayan Mashahreh,Fouad Hassouna,Asaf Shilo,Mohammad Abu-Odeh,Michael Berger,Reuven Wiener,Rami Aqeilan,Tamar Geiger,Rotem Karni

Neural regeneration research 17:163-169 PubMed34100452

2021

Neat1 decreases neuronal apoptosis after oxygen and glucose deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Na Chai,Yi-Fan Wu,Zhi-Min Wu,Yan-Feng Xie,Quan-Hong Shi,Wei Dan,Yan Zhan,Jian-Jun Zhong,Wei Tang,Xiao-Chuan Sun,Li Jiang

Cell communication and signaling : CCS 18:153 PubMed32948206

2020

Rabies virus phosphoprotein P5 binding to BECN1 regulates self-replication by BECN1-mediated autophagy signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Liu,Min Liao,Yan Yan,Hui Yang,Hailong Wang,Jiyong Zhou

International journal of molecular sciences 21: PubMed32785008

2020

The Essential Role of Peptidylarginine Deiminases 2 for Cytokines Secretion, Apoptosis, and Cell Adhesion in Macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Chun Yu,Chien-Hsueh Tung,Kuang-Yung Huang,Hsien-Bin Huang,Ming-Chi Lu

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 52:314-323 PubMed31607566

2019

L-carnitine supplementation attenuates NAFLD progression and cardiac dysfunction in a mouse model fed with methionine and choline-deficient diet.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Mollica,Pamela Senesi,Roberto Codella,Fernanda Vacante,Anna Montesano,Livio Luzi,Ileana Terruzzi

Cell 178:302-315.e23 PubMed31299200

2019

The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Zierhut,Norihiro Yamaguchi,Maria Paredes,Ji-Dung Luo,Thomas Carroll,Hironori Funabiki

Nature methods 15:909-912 PubMed30377371

2018

A high-throughput pipeline for validation of antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Sikorski,Adi Mehta,Marit Inngjerdingen,Flourina Thakor,Simon Kling,Tomas Kalina,Tuula A Nyman,Maria Ekman Stensland,Wei Zhou,Gustavo A de Souza,Lars Holden,Jan Stuchly,Markus Templin,Fridtjof Lund-Johansen

Experimental and therapeutic medicine 14:4104-4110 PubMed29104628

2017

Renal sympathetic denervation improves myocardial apoptosis in rats with isoproterenol-induced heart failure by downregulation of tumor necrosis factor-α and nuclear factor-κB.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yao,Neng Wang,Jin Qian,Lu Bai,Xiaoxin Zheng,Guo Hou,Xuan Qiu,Bo Yang

Autophagy 13:739-753 PubMed28129024

2017

BECN1-dependent CASP2 incomplete autophagy induction by binding to rabies virus phosphoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Liu,Hailong Wang,Jinyan Gu,Tingjuan Deng,Zhuangchuan Yuan,Boli Hu,Yunbin Xu,Yan Yan,Jie Zan,Min Liao,Erin DiCaprio,Jianrong Li,Shuo Su,Jiyong Zhou
View all publications
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