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Rabbit Recombinant Monoclonal Caspase-2 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 9 publications.

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Images

Western blot - Anti-Caspase-2 antibody [EPR16796] (AB179520), expandable thumbnail
  • Western blot - Anti-Caspase-2 antibody [EPR16796] (AB179520), expandable thumbnail
  • Western blot - Anti-Caspase-2 antibody [EPR16796] (AB179520), expandable thumbnail
  • Western blot - Anti-Caspase-2 antibody [EPR16796] (AB179520), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival (PubMed:15073321). Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (PubMed:15073321).

Alternative names

Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, Protease ICH-1, NEDD-2, CASP2, ICH1, NEDD2

Recommended products

Rabbit Recombinant Monoclonal Caspase-2 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 9 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR16796

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520), expandable thumbnail

    Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: Caspase-2 knockout HAP1 cell lysate (20 μg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab17952 observed at 51 kDa. Red - loading control, Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226, observed at 42 kDa.

    ab179520 was shown to recognize Caspase-2 when Caspase-2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase-2 knockout samples were subjected to SDS-PAGE. ab179520 and Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)

    Predicted band size: 51 kDa

  • Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520), expandable thumbnail

    Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)

    The 33KDa band (cleaved caspase 2) contains subunit p18.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 4uM camptothecin for 4 hours at 10 µg

    Lane 2: Untreated HeLa whole cell lysate at 10 µg

    Lane 3: C6 (Rat glial tumor cells) whole cell lysate treated with 1uM staurosporine for 4 hours at 10 µg

    Lane 4: Untreated C6 whole cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa

    Observed band size: 33 kDa, 48 kDa

    Exposure time: 3min

  • Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520), expandable thumbnail

    Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)

    The 33KDa band (cleaved caspase 2) contains subunit p18.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/1000 dilution

    All lanes: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa

    Observed band size: 33 kDa, 48 kDa

    Exposure time: 3min

  • Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520), expandable thumbnail

    Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)

    The 33KDa band (cleaved caspase 2) contains subunit p18.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilution

    All lanes: Human fetal kidney tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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