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Rabbit Recombinant Monoclonal Caspase-2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (AB240217), expandable thumbnail
  • Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (AB240217), expandable thumbnail
  • Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (AB240217), expandable thumbnail
  • Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (AB240217), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Is a regulator of the cascade of caspases responsible for apoptosis execution (PubMed:11156409, PubMed:15073321, PubMed:8087842). Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival (PubMed:15073321). Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (PubMed:15073321).Isoform 1Acts as a positive regulator of apoptosis.Isoform 2Acts as a negative regulator of apoptosis.Isoform 3May function as an endogenous apoptosis inhibitor that antagonizes caspase activation and cell death.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Caspase-2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR16796

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab240217 is the carrier-free version of Anti-Caspase-2 antibody [EPR16796] ab179520.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217), expandable thumbnail

    Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)

    This data was developed using Anti-Caspase-2 antibody [EPR16796] ab179520, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 Âμg)

    Lane 2: Caspase-2 knockout HAP1 cell lysate (20 Âμg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab17952 observed at 51 kDa. Red - loading control, Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226, observed at 42 kDa.

    Anti-Caspase-2 antibody [EPR16796] ab179520 was shown to recognize Caspase-2 when Caspase-2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase-2 knockout samples were subjected to SDS-PAGE. Anti-Caspase-2 antibody [EPR16796] ab179520 and Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Caspase-2 antibody [EPR16796] (Anti-Caspase-2 antibody [EPR16796] ab179520)

    Predicted band size: 51 kDa

  • Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217), expandable thumbnail

    Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)

    This data was developed using Anti-Caspase-2 antibody [EPR16796] ab179520, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The 33KDa band (cleaved caspase 2) contains subunit p18.

    All lanes: Western blot - Anti-Caspase-2 antibody [EPR16796] (Anti-Caspase-2 antibody [EPR16796] ab179520) at 1/2000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 4uM camptothecin for 4 hours at 10 µg

    Lane 2: Untreated HeLa whole cell lysate at 10 µg

    Lane 3: C6 (Rat glial tumor cells) whole cell lysate treated with 1uM staurosporine for 4 hours at 10 µg

    Lane 4: Untreated C6 whole cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa

    Observed band size: 33 kDa, 48 kDa

    Exposure time: 3min

  • Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217), expandable thumbnail

    Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)

    This data was developed using Anti-Caspase-2 antibody [EPR16796] ab179520, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The 33KDa band (cleaved caspase 2) contains subunit p18.

    All lanes: Western blot - Anti-Caspase-2 antibody [EPR16796] (Anti-Caspase-2 antibody [EPR16796] ab179520) at 1/1000 dilution

    All lanes: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa

    Observed band size: 33 kDa, 48 kDa

    Exposure time: 3min

  • Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217), expandable thumbnail

    Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)

    This data was developed using Anti-Caspase-2 antibody [EPR16796] ab179520, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The 33KDa band (cleaved caspase 2) contains subunit p18.

    All lanes: Western blot - Anti-Caspase-2 antibody [EPR16796] (Anti-Caspase-2 antibody [EPR16796] ab179520) at 1/2000 dilution

    All lanes: Human fetal kidney tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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