Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (AB240217)

This data was developed using ab179520, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The 33KDa band (cleaved caspase 2) contains subunit p18.

All lanes:

Western blot - Anti-Caspase-2 antibody [EPR16796] (<a href='/en-us/products/primary-antibodies/caspase-2-antibody-epr16796-ab179520'>ab179520</a>) at 1/2000 dilution

All lanes:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 51 kDa

false

Exposure time: 3min

• WB

Lab

Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (AB240217)

This data was developed using ab179520, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 Âμg)

Lane 2 : Caspase-2 knockout HAP1 cell lysate (20 Âμg)

Lanes 1 and 2 : Merged signal (red and green). Green - ab17952 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.

ab179520 was shown to recognize Caspase-2 when Caspase-2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase-2 knockout samples were subjected to SDS-PAGE. ab179520 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Caspase-2 antibody [EPR16796] (<a href='/en-us/products/primary-antibodies/caspase-2-antibody-epr16796-ab179520'>ab179520</a>)

Predicted band size: 51 kDa

false

• WB

Supplier Data

Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (AB240217)

This data was developed using ab179520, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The 33KDa band (cleaved caspase 2) contains subunit p18.

All lanes:

Western blot - Anti-Caspase-2 antibody [EPR16796] (<a href='/en-us/products/primary-antibodies/caspase-2-antibody-epr16796-ab179520'>ab179520</a>) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 4uM camptothecin for 4 hours at 10 µg

Lane 2:

Untreated HeLa whole cell lysate at 10 µg

Lane 3:

C6 (Rat glial tumor cells) whole cell lysate treated with 1uM staurosporine for 4 hours at 10 µg

Lane 4:

Untreated C6 whole cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 33 kDa,48 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (AB240217)

This data was developed using ab179520, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The 33KDa band (cleaved caspase 2) contains subunit p18.

All lanes:

Western blot - Anti-Caspase-2 antibody [EPR16796] (<a href='/en-us/products/primary-antibodies/caspase-2-antibody-epr16796-ab179520'>ab179520</a>) at 1/1000 dilution

All lanes:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 33 kDa,48 kDa

false

Exposure time: 3min