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AB182738

Anti-Caspase-2 antibody [EPR17881]

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(1 Publication)

Rabbit Recombinant Monoclonal Caspase-2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ICH1, NEDD2, CASP2, Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, Protease ICH-1, NEDD-2

4 Images
Western blot - Anti-Caspase-2 antibody [EPR17881] (AB182738)
  • WB

Supplier Data

Western blot - Anti-Caspase-2 antibody [EPR17881] (AB182738)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/2000 dilution

Lane 1:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate treated with 25uM etoposide for 5 hours at 10 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate, untreated at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

false

Exposure time: 3min

Western blot - Anti-Caspase-2 antibody [EPR17881] (AB182738)
  • WB

Lab

Western blot - Anti-Caspase-2 antibody [EPR17881] (AB182738)

Lane 1 : Wild-type HAP1 cell lysate (20 μg).
Lane 2 : Caspase 2 knockout HAP1 cell lysate (20 μg).
Lanes 1 and 2 : Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.

ab182738 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab182738 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)

Predicted band size: 51 kDa

false

Western blot - Anti-Caspase-2 antibody [EPR17881] (AB182738)
  • WB

Supplier Data

Western blot - Anti-Caspase-2 antibody [EPR17881] (AB182738)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/1000 dilution

All lanes:

Human thymus tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 51 kDa

false

Exposure time: 3min

Western blot - Anti-Caspase-2 antibody [EPR17881] (AB182738)
  • WB

Supplier Data

Western blot - Anti-Caspase-2 antibody [EPR17881] (AB182738)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/2000 dilution

Lane 1:

HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysates at 10 µg

Lane 2:

SK-BR-3 (Human mammary gland adenocarcinoma) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17881

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Is a regulator of the cascade of caspases responsible for apoptosis execution (PubMed : 11156409, PubMed : 15073321, PubMed : 8087842). Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival (PubMed : 15073321). Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (PubMed : 15073321).. Isoform 1. Acts as a positive regulator of apoptosis.. Isoform 2. Acts as a negative regulator of apoptosis.. Isoform 3. May function as an endogenous apoptosis inhibitor that antagonizes caspase activation and cell death.
See full target information CASP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature methods 15:909-912 PubMed30377371

2018

A high-throughput pipeline for validation of antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Sikorski,Adi Mehta,Marit Inngjerdingen,Flourina Thakor,Simon Kling,Tomas Kalina,Tuula A Nyman,Maria Ekman Stensland,Wei Zhou,Gustavo A de Souza,Lars Holden,Jan Stuchly,Markus Templin,Fridtjof Lund-Johansen
View all publications
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Product promise

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