Rabbit Recombinant Monoclonal Caspase-2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
IgG
Rabbit
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | |
---|---|
Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Select an associated product type
Is a regulator of the cascade of caspases responsible for apoptosis execution (PubMed:11156409, PubMed:15073321, PubMed:8087842). Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival (PubMed:15073321). Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (PubMed:15073321).Isoform 1Acts as a positive regulator of apoptosis.Isoform 2Acts as a negative regulator of apoptosis.Isoform 3May function as an endogenous apoptosis inhibitor that antagonizes caspase activation and cell death.
ICH1, NEDD2, CASP2, ICH1, NEDD2, Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, Protease ICH-1, NEDD-2
Rabbit Recombinant Monoclonal Caspase-2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
IgG
Rabbit
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR17881
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Lane 1: Wild-type HAP1 cell lysate (20 μg).
Lane 2: Caspase 2 knockout HAP1 cell lysate (20 μg).
Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226, observed at 42 kDa.
ab182738 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab182738 and Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)
Predicted band size: 51 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/2000 dilution
Lane 1: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate treated with 25uM etoposide for 5 hours at 10 µg
Lane 2: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate, untreated at 10 µg
All lanes: Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Exposure time: 3min
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/2000 dilution
Lane 1: HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysates at 10 µg
Lane 2: SK-BR-3 (Human mammary gland adenocarcinoma) whole cell lysates at 10 µg
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Exposure time: 3min
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/1000 dilution
All lanes: Human thymus tissue lysate at 10 µg
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 51 kDa
Exposure time: 3min
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