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Rabbit Recombinant Monoclonal Caspase-2 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications.

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Images

Western blot - Anti-Caspase-2 antibody [Y154] (AB32021), expandable thumbnail
  • Western blot - Anti-Caspase-2 antibody [Y154] (AB32021), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species

Human

Dilution info

1/500

Notes

Detects bands of approximately 50 kDa (pro form) and 18 kDa (p18 cleaved form).

Associated Products

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Target data

Function

Is a regulator of the cascade of caspases responsible for apoptosis execution (PubMed:11156409, PubMed:15073321, PubMed:8087842). Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival (PubMed:15073321). Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (PubMed:15073321).Isoform 1Acts as a positive regulator of apoptosis.Isoform 2Acts as a negative regulator of apoptosis.Isoform 3May function as an endogenous apoptosis inhibitor that antagonizes caspase activation and cell death.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Caspase-2 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

Y154

Purification technique

Affinity purification Protein A

Specificity

This antibody is specific for the pro form and the p18 cleaved form of Caspase-2.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Caspase-2 antibody [Y154] (ab32021), expandable thumbnail

    Western blot - Anti-Caspase-2 antibody [Y154] (ab32021)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: Caspase 2 knockout HAP1 cell lysate (20 μg)

    Lanes 1 and 2: Merged signal (red and green). Green - Anti-Caspase-2 antibody [EPR17881] ab182738 observed at 51 kDa. Red - loading control, Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226, observed at 42 kDa.

    ab32021 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab32021 and Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 (loading control to beta actin) were diluted 1/50 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Caspase-2 antibody [Y154] (ab32021)

    Predicted band size: 51 kDa

  • Western blot - Anti-Caspase-2 antibody [Y154] (ab32021), expandable thumbnail

    Western blot - Anti-Caspase-2 antibody [Y154] (ab32021)

    All lanes: Western blot - Anti-Caspase-2 antibody [Y154] (ab32021) at 1/500 dilution

    All lanes: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Predicted band size: 51 kDa

    Observed band size: 18 kDa, 50 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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