Rabbit Recombinant Monoclonal Caspase-2 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
Liquid
Monoclonal
WB | |
---|---|
Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes Detects bands of approximately 50 kDa (pro form) and 18 kDa (p18 cleaved form). |
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Is a regulator of the cascade of caspases responsible for apoptosis execution (PubMed:11156409, PubMed:15073321, PubMed:8087842). Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival (PubMed:15073321). Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (PubMed:15073321).Isoform 1Acts as a positive regulator of apoptosis.Isoform 2Acts as a negative regulator of apoptosis.Isoform 3May function as an endogenous apoptosis inhibitor that antagonizes caspase activation and cell death.
ICH1, NEDD2, CASP2, ICH1, NEDD2, Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, Protease ICH-1, NEDD-2
Rabbit Recombinant Monoclonal Caspase-2 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
Liquid
Monoclonal
Y154
Affinity purification Protein A
This antibody is specific for the pro form and the p18 cleaved form of Caspase-2.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Caspase 2 knockout HAP1 cell lysate (20 μg)
Lanes 1 and 2: Merged signal (red and green). Green - Anti-Caspase-2 antibody [EPR17881] ab182738 observed at 51 kDa. Red - loading control, Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226, observed at 42 kDa.
ab32021 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab32021 and Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 (loading control to beta actin) were diluted 1/50 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Caspase-2 antibody [Y154] (ab32021)
Predicted band size: 51 kDa
All lanes: Western blot - Anti-Caspase-2 antibody [Y154] (ab32021) at 1/500 dilution
All lanes: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Predicted band size: 51 kDa
Observed band size: 18 kDa, 50 kDa
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