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AB247211

Anti-Caspase-2 antibody [Y154] - BSA and Azide free

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Rabbit Recombinant Monoclonal Caspase-2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

ICH1, NEDD2, CASP2, Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, Protease ICH-1, NEDD-2

2 Images
Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (AB247211)
  • WB

Unknown

Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (AB247211)

This data was developed using ab32021, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Caspase-2 antibody [Y154] (<a href='/en-us/products/primary-antibodies/caspase-2-antibody-y154-ab32021'>ab32021</a>) at 1/500 dilution

All lanes:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Predicted band size: 51 kDa

Observed band size: 18 kDa,50 kDa

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Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (AB247211)
  • WB

Lab

Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (AB247211)

This data was developed using ab32021, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)

Lane 2 : Caspase 2 knockout HAP1 cell lysate (20 μg)

Lanes 1 and 2 : Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.

ab32021 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab32021 and ab8226 (loading control to beta actin) were diluted 1/50 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Caspase-2 antibody [Y154] (<a href='/en-us/products/primary-antibodies/caspase-2-antibody-y154-ab32021'>ab32021</a>)

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y154

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific for the pro form and the p18 cleaved form of Caspase-2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Detects bands of approximately 50 kDa (pro form) and 18 kDa (p18 cleaved form).</p>" } } }
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Product details

ab247211 is the carrier-free version of ab32021.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Is a regulator of the cascade of caspases responsible for apoptosis execution (PubMed : 11156409, PubMed : 15073321, PubMed : 8087842). Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival (PubMed : 15073321). Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (PubMed : 15073321).. Isoform 1. Acts as a positive regulator of apoptosis.. Isoform 2. Acts as a negative regulator of apoptosis.. Isoform 3. May function as an endogenous apoptosis inhibitor that antagonizes caspase activation and cell death.
See full target information CASP2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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