Anti-Caspase-2L antibody [EPR16790]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Caspase-2L antibody [EPR16790] (AB179519)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Caspase-2L with ab179519 at 1/500 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
1. ab179519 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-2L antibody [EPR16790] (AB179519)

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Caspase-2L using ab179519 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain : Hematoxylin.
Inset image : negative control obtained using PBS instead of ab179519.
Note : nuclear staining on the tumor cells of Human lung cancer was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-2L antibody [EPR16790] (AB179519)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Caspase-2L using ab179519 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain : Hematoxylin.
Inset image : negative control obtained using PBS instead of ab179519.
Note : nuclear staining on the germ cells of the Human testis was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Caspase-2L antibody [EPR16790] (AB179519)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedJurkat (Human T cell leukemia cells from peripheral blood) cells labeling Caspase-2 with ab179519 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

• WB

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Western blot - Anti-Caspase-2L antibody [EPR16790] (AB179519)

Blocking/Dilution buffer : 5% NFDM/TBST.

Observed MW : 48,12 kDa

All lanes:

Western blot - Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/10000 dilution

All lanes:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Caspase-2L antibody [EPR16790] (AB179519)

Blocking/Dilution buffer : 5% NFDM/TBST.

Observed MW : 48, 30, 14, 12 kDa

All lanes:

Western blot - Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/2000 dilution

Lane 1:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates treated with 1uM staurosporine for 4 hours at 10 µg

Lane 2:

untreated Jurkat whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Caspase-2L antibody [EPR16790] (AB179519)

Blocking/Dilution buffer : 5% NFDM/TBST.

Observed MW : 48,30,12 kDa

All lanes:

Western blot - Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/2000 dilution

All lanes:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

false

Exposure time: 3min