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Mouse Monoclonal Caspase-3 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 106 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CASP3.


Images

Western blot - Anti-Caspase-3 antibody [31A1067] (AB13585), expandable thumbnail
  • Western blot - Anti-Caspase-3 antibody [31A1067] (AB13585), expandable thumbnail
  • Western blot - Anti-Caspase-3 antibody [31A1067] (AB13585), expandable thumbnail
  • Western blot - Anti-Caspase-3 antibody [31A1067] (AB13585), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human CASP3. Database link P42574

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Human
Dilution info
1.00000-5.00000 µg/mL
Notes

The antibody detects both pro Caspase 3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase 3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase 3 pro-domain.

Species
Mouse
Dilution info
1.00000-5.00000 µg/mL
Notes

The antibody detects both pro Caspase 3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase 3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase 3 pro-domain.

Species
Rat
Dilution info
1.00000-5.00000 µg/mL
Notes

The antibody detects both pro Caspase 3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase 3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase 3 pro-domain.

Associated Products

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Target data

Function

Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:35338844, PubMed:35446120, PubMed:7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed:10497198, PubMed:16374543, PubMed:7596430, PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed:7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed:37993714, PubMed:9334240). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed:35338844, PubMed:35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758104, PubMed:36758106).

Alternative names

CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1

Recommended products

Mouse Monoclonal Caspase-3 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 106 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CASP3.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human CASP3. Database link P42574
Clone number
31A1067
Purification technique
Affinity purification Protein G
Specificity

ab13585 recognizes an active form of Caspase 3 after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells

Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585), expandable thumbnail

    Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585)

    Lane 1: Wild-type HAP1 cell lysate + Staurosporine (1μM for 4h)
    Lane 2: Wild-type HAP1 cell lysate
    Lane 3: Caspase-3 knockout HAP1 cell lysate + Staurosporine (1μM for 4h)
    Lane 4: Caspase-3 knockout HAP1 cell lysate
    Lanes 1 - 4: Merged signal (red and green). Green - ab13585 observed at 32 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.

    ab13585 was shown to recognise pro Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (± Staurosporine treatment) were subjected to SDS-PAGE. ab13585 at a concentration of 1 μg/ml and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) diluted to 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585)

    Predicted band size: 31 kDa

    Observed band size: 32 kDa

  • Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585), expandable thumbnail

    Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585)

    Western blot analysis of Caspase 3 in HeLa cells using ab13585. Cells were treated with 2 uM staurosporine for different time periods. Caspase 3 activation is detected in Western blots by the presence of Caspase 3 cleavage fragments.

    ab13585 detected both pro (full-length) and active (cleaved) Caspase 3, depending on the treatment time points. Pro Caspase 3 is detected at ~32 kDa. Active/cleaved Caspase 3 (large subunit) is detected at ~14-21 kDa as one or more bands.

    All lanes: Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585)

    Predicted band size: 31 kDa

  • Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585), expandable thumbnail

    Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585)

    Lanes 12, 13 and 14 demonstrate the species cross-reactivity of the antibody in Human, mouse and rat heart lysate, respectively.

    All lanes: Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585) at 5 µg/mL

    Lane 1: Human brain lysate

    Lanes 2 and 12: Human heart lysate

    Lane 3: Human intestine lysate

    Lane 4: Human kidney lysate

    Lane 5: Human liver lysate

    Lane 6: Human lung lysate

    Lane 7: Human muscle lysate

    Lane 8: Human stomach lysate

    Lane 9: Human spleen lysate

    Lane 10: Human ovary lysate

    Lane 11: Human testis lysate

    Lane 13: Mouse heart lysate

    Lane 14: Rat heart lysate

    Predicted band size: 31 kDa

  • Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585), expandable thumbnail

    Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab13585 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    All lanes: Anti-MDC1 antibody (ab13858) at 1 µg/mL

    Lane 1: Hela whole cell lysate (Staurosporine treated, 2uM/4hr) at 20 µg

    Lane 2: Hela whole cell lysate (untreated control) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) ab175781) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 22 kDa, 28 kDa, 29 kDa, 31 kDa, 50 kDa

    Observed band size: 17 kDa, 19 kDa, 32 kDa, 36 kDa, 43 kDa

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