Anti-Caspase-3 antibody is a rabbit polyclonal antibody that is used to detect Caspase-3 in Western blot. Suitable for Human samples.
- Specificity confirmed with CASP3 knockout cell line validation
- Tried and trusted by researchers since 2005
- Cited in over 1190 publications
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- Drug design, development and therapy 17:3103-31282023Sijunzi Decoction Targets IL1B and TNF to Reduce Neutrophil Extracellular Traps (NETs) in Ulcerative Colitis: Evidence from Silicon Prediction and Experiment Validation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Zhang,Siwei Duan,Zhangyou He,Zeming Zhu,Zhiping Li,Qincheng Yi,Tiantian Cai,Juanjuan Li,Nan Chen,Shaoju GuoPubMed 37868820
- Open life sciences 18:202207262023HK2 contributes to the proliferation, migration, and invasion of diffuse large B-cell lymphoma cells by enhancing the ERK1/2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongcan Zhao,Guoqian Xiang,Tingjun Shao,Minmin Wang,Weijian DaiPubMed 37854321
- Renal failure 45:22592342023Butylphthalide improves brain damage induced by renal ischemia-reperfusion injury rats through Nrf2/HO-1 and NOD2/MAPK/NF-κB pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Min,Qi Chen,Mengxiong Pan,Tan Liu,Qun Gu,Dongwei Zhang,Ru SunPubMed 37732403
- Medical oncology (Northwood, London, England) 40:2892023The apoptotic effect of the Lycopodium clavatum extracts on MCF-7 human breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYusuf Kucukbagriacik,Mohammadreza Dastouri,Humeyra Yilmaz,Evrim Gunes AltuntasPubMed 37658180
- World journal of gastroenterology 29:4542-45562023MiR-204-3p overexpression inhibits gastric carcinoma cell proliferation by inhibiting the MAPK pathway and RIP1/MLK1 necroptosis pathway to promote apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXia Li,Joanna J Tibenda,Yi Nan,Shi-Cong Huang,Na Ning,Guo-Qing Chen,Yu-Hua Du,Ya-Ting Yang,Fan-Di Meng,Ling YuanPubMed 37621755
- Biological research 56:482023Mucin1 induced trophoblast dysfunction in gestational diabetes mellitus via Wnt/β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuang-Shuang Cui,Ping Zhang,Lu Sun,Yu-Lin-Lan Yuan,Jingyun Wang,Feng-Xiang Zhang,Ruiman LiPubMed 37608294
- Phytotherapy research : PTR 37:4740-47542023Targeting HSP90 with picropodophyllin suppresses gastric cancer tumorigenesis by disrupting the association of HSP90 and AKT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoli Li,Guoli Wang,Xiaolin Zhou,Huijie Zhao,Xiaojie Chen,Qixiao Cui,Minjing Li,Xihang Gao,Xiaoyu Wei,Lei Ye,Defang Li,Pan HongPubMed 37559472
- Frontiers in molecular neuroscience 16:11820052023Interferon-induced transmembrane protein 3 in the hippocampus: a potential novel target for the therapeutic effects of recombinant human brain natriuretic peptide on sepsis-associated encephalopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Li,Rui-Hang Ma,Er-Fei Zhang,Feng Ge,De-Yu Fang,Jing Zhang,Yan-Ning Zhang,Yan Gao,Li-Chao Hou,Hong-Xu JinPubMed 37602193
- Medical oncology (Northwood, London, England) 40:2282023The apoptotic effects of NK-92 cells stimulated with an anti-CD226 antibody on MDA-MB-231 triple-negative breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammadreza Dastouri,Nil Kilic,Humeyra YilmazPubMed 37410214
- Immunity, inflammation and disease 11:e9042023Epimedium sagittatum Maxim ameliorates adriamycin-induced nephropathy by restraining inflammation and apoptosis via the PI3K/AKT signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRu Wang,Mengnan Zeng,Beibei Zhang,Qinqin Zhang,Shuangshuang Xie,Yingbo Hu,Ruyi Fan,Mengya Wang,Xiao Yu,Yuhan Zhang,Xiaoke Zheng,Weisheng FengPubMed 37382268
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Chinese hamster | Predicted |
| Common marmoset | Predicted |
| Dog | Predicted |
| Drosophila melanogaster | Predicted |
| Indian muntjac | Predicted |
| Pig | Predicted |
| Rhesus monkey | Predicted |
| Salvelinus alpinus | Predicted |
| Schmidtea mediterranea | Predicted |
| Xenopus laevis | Predicted |
| Zebrafish | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Schmidtea mediterranea, Salvelinus alpinus, Mouse, Rat, Dog, Pig, Xenopus laevis, Drosophila melanogaster, Indian muntjac, Zebrafish, Rhesus monkey, Chinese hamster, Common marmoset | Dilution info - | Notes - |
Associated Products
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Target data
Function
Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:35338844, PubMed:35446120, PubMed:7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed:10497198, PubMed:16374543, PubMed:7596430, PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed:7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed:37993714, PubMed:9334240). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed:35338844, PubMed:35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758104, PubMed:36758106).
Alternative names
CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1
Recommended products
Anti-Caspase-3 antibody is a rabbit polyclonal antibody that is used to detect Caspase-3 in Western blot. Suitable for Human samples.
- Specificity confirmed with CASP3 knockout cell line validation
- Tried and trusted by researchers since 2005
- Cited in over 1190 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Stimulation is required to allow detection of the 17kDa cleaved form of the protein. Please see images below for recommended treatment conditions and positive controls.
ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells.
Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. We would recommend ab32351 and ab184787 as an alternative product for this application.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Caspase-3 antibody (ab13847) is a rabbit polyclonal antibody and is validated for use in WB in human samples.
Anti-Caspase-3 antibody (ab13847) has been cited over 1194 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Caspase-3 antibody (ab13847) has high sensitivity and specificity.
The specificity of Anti-Caspase-3 antibody (ab13847) has been confirmed by testing in knockout samples.
Anti-Caspase-3 antibody (ab13847) has 53 independent reviews from customers.
Anti-Caspase-3 antibody (ab13847) specifically detects Caspase-3 (UniProt ID: P42574; Molecular weight: 17kDa) and is sold in 100 µg selling sizes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Caspase-3 antibody (ab13847)
Caspase 3 exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce large (17kDa) and small (12kDa) subunits. These subunits dimerize to form the active enzyme. ab13847 specifically detects the large active subunit (17kDa) and the inactive pro Caspase 3 (32 kDa).
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab13847 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Secondary antibody - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody
All lanes: Western blot - Anti-Caspase-3 antibody (ab13847) at 1 µg/mL
Lane 1: Human Caspase 3 (active) Recombinant Protein at 0.1 µg
Lane 2: Human Pro Caspase 3 (inactive) Recombinant Protein at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 17 kDa, 32 kDa
Exposure time: 8min
Western blot - Anti-Caspase-3 antibody (ab13847)
Lane 1: Wild-type HAP1 cell lysate + Staurosporine (Staurosporine (DMSO solution), protein kinase inhibitor ab146588) (1μM for 4h)
Lane 2: Wild-type HAP1 cell lysate
Lane 3: Caspase-3 knockout HAP1 cell lysate + Staurosporine (Staurosporine (DMSO solution), protein kinase inhibitor ab146588) (1μM for 4h)
Lane 4: Caspase-3 knockout HAP1 cell lysateLanes 1 - 4: Merged signal (red and green). Green - ab13847 observed at 17 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE. ab13847 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Caspase-3 antibody (ab13847)
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 26 kDa, 34 kDa
This image is courtesy of an anonymous customer reviewWestern blot - Anti-Caspase-3 antibody (ab13847)
4-20% (Mini protean gels) non-denaturing conditions
All lanes: Western blot - Anti-Caspase-3 antibody (ab13847) at 1/1000 dilution
Lane 1: Skin (atopic dermatits) whole cell lysate at 20 µg
Lane 2: Skin (without atopic dermatits) whole cell lysate at 20 µg
SecondaryAll lanes: Goat anti rabbit HRP at 1/2000 dilution
Performed under reducing conditions.
Exposure time: 5min
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