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Anti-Caspase-3 antibody is a rabbit polyclonal antibody that is used to detect Caspase-3 in Western blot. Suitable for Human samples.

- Specificity confirmed with CASP3 knockout cell line validation
- Tried and trusted by researchers since 2005
- Cited in over 1190 publications


Images

Western blot - Anti-Caspase-3 antibody (AB13847), expandable thumbnail
  • Western blot - Anti-Caspase-3 antibody (AB13847), expandable thumbnail
  • Western blot - Anti-Caspase-3 antibody (AB13847), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form
Liquid
Clonality
Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted
Chinese hamster
Predicted
Common marmoset
Predicted
Dog
Predicted
Drosophila melanogaster
Predicted
Indian muntjac
Predicted
Pig
Predicted
Rhesus monkey
Predicted
Salvelinus alpinus
Predicted
Schmidtea mediterranea
Predicted
Xenopus laevis
Predicted
Zebrafish
Predicted

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Predicted
Predicted

Species
Schmidtea mediterranea, Salvelinus alpinus, Mouse, Rat, Dog, Pig, Xenopus laevis, Drosophila melanogaster, Indian muntjac, Zebrafish, Rhesus monkey, Chinese hamster, Common marmoset
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:35338844, PubMed:35446120, PubMed:7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed:10497198, PubMed:16374543, PubMed:7596430, PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed:7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed:37993714, PubMed:9334240). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed:35338844, PubMed:35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758104, PubMed:36758106).

Alternative names

Recommended products

Anti-Caspase-3 antibody is a rabbit polyclonal antibody that is used to detect Caspase-3 in Western blot. Suitable for Human samples.

- Specificity confirmed with CASP3 knockout cell line validation
- Tried and trusted by researchers since 2005
- Cited in over 1190 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique
Affinity purification Immunogen
Specificity

Stimulation is required to allow detection of the 17kDa cleaved form of the protein. Please see images below for recommended treatment conditions and positive controls.

ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells.

Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. We would recommend ab32351 and ab184787 as an alternative product for this application.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-Caspase-3 antibody (ab13847) is a rabbit polyclonal antibody and is validated for use in WB in human samples.

Anti-Caspase-3 antibody (ab13847) has been cited over 1194 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Caspase-3 antibody (ab13847) has high sensitivity and specificity.

The specificity of Anti-Caspase-3 antibody (ab13847) has been confirmed by testing in knockout samples.

Anti-Caspase-3 antibody (ab13847) has 53 independent reviews from customers.

Anti-Caspase-3 antibody (ab13847) specifically detects Caspase-3 (UniProt ID: P42574; Molecular weight: 17kDa) and is sold in 100 µg selling sizes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Caspase-3 antibody (ab13847), expandable thumbnail

    Western blot - Anti-Caspase-3 antibody (ab13847)

    Caspase 3 exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce large (17kDa) and small (12kDa) subunits. These subunits dimerize to form the active enzyme. ab13847 specifically detects the large active subunit (17kDa) and the inactive pro Caspase 3 (32 kDa).

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab13847 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Secondary antibody - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody

    All lanes: Western blot - Anti-Caspase-3 antibody (ab13847) at 1 µg/mL

    Lane 1: Human Caspase 3 (active) Recombinant Protein at 0.1 µg

    Lane 2: Human Pro Caspase 3 (inactive) Recombinant Protein at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 31 kDa

    Observed band size: 17 kDa, 32 kDa

    Exposure time: 8min

  • Western blot - Anti-Caspase-3 antibody (ab13847), expandable thumbnail

    Western blot - Anti-Caspase-3 antibody (ab13847)

    Lane 1: Wild-type HAP1 cell lysate + Staurosporine (Staurosporine (DMSO solution), protein kinase inhibitor ab146588) (1μM for 4h)
    Lane 2: Wild-type HAP1 cell lysate
    Lane 3: Caspase-3 knockout HAP1 cell lysate + Staurosporine (Staurosporine (DMSO solution), protein kinase inhibitor ab146588) (1μM for 4h)
    Lane 4: Caspase-3 knockout HAP1 cell lysate

    Lanes 1 - 4: Merged signal (red and green). Green - ab13847 observed at 17 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE. ab13847 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Caspase-3 antibody (ab13847)

    Performed under reducing conditions.

    Predicted band size: 31 kDa

    Observed band size: 26 kDa, 34 kDa

  • Western blot - Anti-Caspase-3 antibody (ab13847), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-Caspase-3 antibody (ab13847)

    4-20% (Mini protean gels) non-denaturing conditions

    All lanes: Western blot - Anti-Caspase-3 antibody (ab13847) at 1/1000 dilution

    Lane 1: Skin (atopic dermatits) whole cell lysate at 20 µg

    Lane 2: Skin (without atopic dermatits) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat anti rabbit HRP at 1/2000 dilution

    Performed under reducing conditions.

    Exposure time: 5min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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